Enhancement of lycopene extraction from tomatoes using pulsed electric field

A study was conducted to evaluate the effect of pulsed electric field (PEF) treatment for enhancing lycopene extraction. Tomato pulp was obtained by blending fresh tomato fruit. Approximately 5 g of the pulp was subjected to PEF. The parameters of the treatments were: electric field 4, 8, 16, 20 and 24 kV/cm, capacity of discharge capacitor 0.02, 0.06, 1, 2 and 3 μF and number of pulses 10, 20, 30, 40 and 50. Lycopene content was determined using spectrophotometric method at the wavelength of 503 nm after low volume hexane extraction. PEF treatment increased lycopene extraction from fresh tomato fruit generally. A maximum of 68.8% was obtained using 16 kV/cm, 2 μF, and 50 pulses compared to untreated samples, however 20 kV/cm, 0.06μF, 50 pulses was shown to be more energy efficient.

[1]  D. Barrett,et al.  LYCOPENE CONTENT OF CALIFORNIA-GROWN TOMATO VARIETIES , 2001 .

[2]  E. Williams,et al.  Steady-state membrane hyperpolarization by large applied currents in Nitella translucens. , 1968, Biophysical Journal.

[3]  John Shi,et al.  Lycopene in Tomatoes: Chemical and Physical Properties Affected by Food Processing , 2000, Critical reviews in biotechnology.

[4]  M. Ngadi,et al.  Drying Characteristics of Pulsed Electric Field-Treated Carrot , 2008 .

[5]  Herbert Dyson Carter,et al.  Electric discharges in liquids. Part I—the arc discharge in water , 1932 .

[6]  M. Ngadi,et al.  International Journal of Food Engineering Inactivation of Escherichia Coli 0157 : H 7 and Salmonella Enteritidis in Liquid Egg Using Continuous Pulsed Electric Field System , 2011 .

[7]  O. Kooten,et al.  Effects of cutting and maturity on lycopene concentration of fresh-cut tomatoes during storage at different temperatures , 2005 .

[8]  B. Watkins,et al.  Supercritical fluid extraction of lycopene from tomato processing byproducts. , 2002, Journal of agricultural and food chemistry.

[9]  G. Barbosa‐Cánovas,et al.  Inactivation of Listeria innocua in skim milk by pulsed electric fields and nisin. , 1999, International journal of food microbiology.

[10]  E. M. D. L. Ossa,et al.  Recovery of grape seed oil by liquid and supercritical carbon dioxide extraction: a comparison with conventional solvent extraction , 1996 .

[11]  E. Vorobiev,et al.  Kinetic model of sugar diffusion from sugar beet tissue treated by pulsed electric field , 2005 .

[12]  Penelope Perkins-Veazie,et al.  A Quantitative Assay for Lycopene That Utilizes Reduced Volumes of Organic Solvents , 2002 .

[13]  Eugène Vorobiev,et al.  Pulsed Electric Field Assisted Pressing of Sugar Beet Slices: towards a Novel Process of Cold Juice Extraction , 2006 .

[14]  A. Rao,et al.  Tomato lycopene and low density lipoprotein oxidation: A human dietary intervention study , 1998, Lipids.

[15]  Penelope Perkins-Veazie,et al.  Lycopene content differs among red‐fleshed watermelon cultivars , 2001 .

[16]  L. Zechmeister,et al.  cis-trans Isomerization and Spectral Characteristics of Carotenoids and Some Related Compounds , 1943 .

[17]  E. Vorobiev,et al.  Pulsed electric field treatment of apple tissue during compression for juice extraction , 2001 .

[18]  M. Raghavan,et al.  Pulsed electric field assisted juice extraction from alfalfa , 2006 .

[19]  H. Coster,et al.  A quantitative analysis of the voltage-current relationships of fixed charge membranes and the associated property of "punch-through". , 1965, Biophysical journal.

[20]  G. S. Siddappa,et al.  A rapid spectrophotometric method for the detection and estimation of adulterants in tomato ketchup. , 1959 .

[21]  T. Baysal,et al.  Supercritical CO2 Extraction of β-Carotene and Lycopene from Tomato Paste Waste , 2000 .

[22]  Volker Heinz,et al.  Preservation of liquid foods by high intensity pulsed electric fields-basic concepts for process design , 2001 .

[23]  T. Baysal,et al.  Supercritical CO(2) extraction of beta-carotene and lycopene from tomato paste waste. , 2000, Journal of agricultural and food chemistry.

[24]  D. Knorr,et al.  High electric field pulse pretreatment: potential for sugar beet processing , 2002 .