Aromatic Surfactant as Aggregating Agent for Aptamer-Gold Nanoparticle-Based Detection of Plasmodium Lactate Dehydrogenase

A novel ssDNA aptamer (P38), with a 40 mer random region flanked by primer-binding sites on both sides, targeting Plasmodium falciparum lactate dehydrogenase (PfLDH) has been developed through systematic evolution of ligands by exponential enrichment (SELEX), including counter SELEX against human lactate dehydrogenase A and B (hLDH A and B). The 2D structure of P38 shows the presence of three stem loops with a δG of −9.18 kcal/mol. EMSA studies on P38 complexes with the increasing concentration of PfLDH revealed a dissociation constant of 0.35 µM. P38 has been exploited for the quantitative detection of PfLDH using cationic surfactant-mediated aggregation of gold nanoparticles (16-nm diameter) as an optical probe. Among the three different cationic surfactants, characterized by different hydrocarbon tail groups, benzalkonium chloride (BCK) was found to be most efficient with a limit of detection of 281 ± 11 pM. This BCK-based approach with the novel highly selective aptamer provides simple and sensitive detection of PfLDH in the clinically relevant range.

[1]  B. Korgel,et al.  The importance of the CTAB surfactant on the colloidal seed-mediated synthesis of gold nanorods. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[2]  G. Sabato,et al.  The Denaturation of Lactic Dehydrogenases , 2003 .

[3]  R. Miller,et al.  One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[4]  E. Vesell.  Formation of human lactate dehydrogenase isozyme patterns in vitro. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[5]  J. Szostak,et al.  In vitro selection of RNA molecules that bind specific ligands , 1990, Nature.

[6]  R. L. Brady,et al.  Structure of lactate dehydrogenase from Plasmodium vivax: complexes with NADH and APADH. , 2005, Biochemistry.

[7]  George Awinda,et al.  Unified parasite lactate dehydrogenase and histidine-rich protein ELISA for quantification of Plasmodium falciparum. , 2009, The American journal of tropical medicine and hygiene.

[8]  Julian A. Tanner,et al.  Structural basis for discriminatory recognition of Plasmodium lactate dehydrogenase by a DNA aptamer , 2013, Proceedings of the National Academy of Sciences.

[9]  B. Hamainza,et al.  Early results of integrated malaria control and implications for the management of fever in under-five children at a peripheral health facility: a case study of Chongwe rural health centre in Zambia , 2009, Malaria Journal.

[10]  N. Kaplan,et al.  The Comparative Enzymology of Lactic Dehydrogenases III. PROPERTIES OF THE H4 AND M4 ENZYMES FROM A NUMBER OF VERTEBRATES , 1967 .

[11]  Charles Tanford,et al.  Physical Chemistry of Macromolecules , 1961 .

[12]  L. Gold,et al.  Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. , 1990, Science.

[13]  Shenshan Zhan,et al.  Ultrasensitive aptamer biosensor for arsenic(III) detection in aqueous solution based on surfactant-induced aggregation of gold nanoparticles. , 2012, The Analyst.

[14]  Wenting Zhi,et al.  A Colorimetric Aptamer Biosensor Based on Gold Nanoparticles for the Ultrasensitive and Specific Detection of Tetracycline in Milk , 2013 .

[15]  Andrew B Kinghorn,et al.  APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria. , 2015, Chemical communications.

[16]  Yi Xiao,et al.  Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes , 2010, Proceedings of the National Academy of Sciences.

[17]  N. Kelley-Loughnane,et al.  Colorimetric detection with aptamer–gold nanoparticle conjugates: effect of aptamer length on response , 2012, Journal of Nanoparticle Research.

[18]  C. Ban,et al.  Gold nanoparticle-based colorimetric detection of kanamycin using a DNA aptamer. , 2011, Analytical biochemistry.

[19]  Sanjukta Patra,et al.  Potential Biomarkers and Their Applications for Rapid and Reliable Detection of Malaria , 2014, BioMed research international.

[20]  J. Kugel,et al.  Using electrophoretic mobility shift assays to measure equilibrium dissociation constants: GAL4‐p53 binding DNA as a model system , 2012, Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology.

[21]  P. Matsudaira,et al.  Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. , 1987, The Journal of biological chemistry.

[22]  Michael Zuker,et al.  Mfold web server for nucleic acid folding and hybridization prediction , 2003, Nucleic Acids Res..

[23]  J. Hillier,et al.  A study of the nucleation and growth processes in the synthesis of colloidal gold , 1951 .

[24]  Wenting Zhi,et al.  Cationic polymers and aptamers mediated aggregation of gold nanoparticles for the colorimetric detection of arsenic(III) in aqueous solution. , 2012, Chemical communications.

[25]  Huixiang Li,et al.  Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[26]  B. Tekwani,et al.  Generation of oxamic acid libraries: antimalarials and inhibitors of Plasmodium falciparum lactate dehydrogenase. , 2007, Journal of combinatorial chemistry.

[27]  Su Jin Lee,et al.  A novel colorimetric aptasensor using gold nanoparticle for a highly sensitive and specific detection of oxytetracycline. , 2010, Biosensors & bioelectronics.

[28]  P.,et al.  The Comparative Enzymology of Lactic Dehydrogenases , 2003 .

[29]  Arthur J. Rowe,et al.  Analytical ultracentrifugation in biochemistry and polymer science , 1992 .

[30]  J. Barnwell,et al.  The heat stability of Plasmodium lactate dehydrogenase-based and histidine-rich protein 2-based malaria rapid diagnostic tests. , 2007, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[31]  Nancy Kelley-Loughnane,et al.  Colorimetric detection with aptamer-gold nanoparticle conjugates coupled to an android-based color analysis application for use in the field. , 2014, Talanta.

[32]  Yoon-Bo Shim,et al.  A highly sensitive aptasensor towards Plasmodium lactate dehydrogenase for the diagnosis of malaria. , 2012, Biosensors & bioelectronics.

[33]  Eun Jeong Cho,et al.  Applications of aptamers as sensors. , 2009, Annual review of analytical chemistry.

[34]  T. Kusunose,et al.  CTAB-Assisted Synthesis of Size- and Shape-Controlled Gold Nanoparticles in SDS Aqueous Solution , 2009 .

[35]  A. Mårtensson,et al.  Short report: Rapid DNA extraction from archive blood spots on filter paper for genotyping of Plasmodium falciparum. , 2005, The American journal of tropical medicine and hygiene.

[36]  C. Ban,et al.  Cationic Surfactant-Based Colorimetric Detection of Plasmodium Lactate Dehydrogenase, a Biomarker for Malaria, Using the Specific DNA Aptamer , 2014, PloS one.

[37]  P. Schuck,et al.  Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling. , 2000, Biophysical journal.

[38]  C. Ban,et al.  A colorimetric aptasensor for the diagnosis of malaria based on cationic polymers and gold nanoparticles. , 2013, Analytical biochemistry.