Near‐field dosimetry for in vitro exposure of human cells at 60 GHz

Due to the expected mass deployment of millimeter-wave wireless technologies, thresholds of potential millimeter-wave-induced biological and health effects should be carefully assessed. The main purpose of this study is to propose, optimize, and characterize a near-field exposure configuration allowing illumination of cells in vitro at 60 GHz with power densities up to several tens of mW/cm(2) . Positioning of a tissue culture plate containing cells has been optimized in the near-field of a standard horn antenna operating at 60 GHz. The optimal position corresponds to the maximal mean-to-peak specific absorption rate (SAR) ratio over the cell monolayer, allowing the achievement of power densities up to 50 mW/cm(2) at least. Three complementary parameters have been determined and analyzed for the exposed cells, namely the power density, SAR, and temperature dynamics. The incident power density and SAR have been computed using the finite-difference time-domain (FDTD) method. The temperature dynamics at different locations inside the culture medium are measured and analyzed for various power densities. Local SAR, determined based on the initial rate of temperature rise, is in a good agreement with the computed SAR (maximal difference of 5%). For the optimized exposure setup configuration, 73% of cells are located within the ±3 dB region with respect to the average SAR. It is shown that under the considered exposure conditions, the maximal power density, local SAR, and temperature increments equal 57 mW/cm(2) , 1.4 kW/kg, and 6 °C, respectively, for the radiated power of 425 mW.

[1]  Marvin C Ziskin,et al.  Low power millimeter wave irradiation exerts no harmful effect on human keratinocytes in vitro , 2003, Bioelectromagnetics.

[2]  A Cowan,et al.  Electromagnetic millimeter wave induced hypoalgesia: Frequency dependence and involvement of endogenous opioids , 2008, Bioelectromagnetics.

[3]  M C Ziskin,et al.  Reflection and penetration depth of millimeter waves in murine skin , 2008, Bioelectromagnetics.

[4]  Jianxun Zhao,et al.  Numerical modeling and dosimetry of the 35 mm Petri dish under 46 GHz millimeter wave exposure. , 2005, Bioelectromagnetics.

[5]  Marvin C Ziskin,et al.  Millimeter wave effects on electrical responses of the sural nerve in vivo. , 2010, Bioelectromagnetics.

[6]  A. Niknejad.  Siliconization of 60 GHz , 2010, IEEE Microwave Magazine.

[7]  Near-field electromagnetic dosimetry for in vitro studies at millimeter waves , 2010, Proceedings of the Fourth European Conference on Antennas and Propagation.

[8]  V. Safronova,et al.  Immunomodulating action of low intensity millimeter waves on primed neutrophils , 2002, Bioelectromagnetics.

[9]  Y. Akyel,et al.  Current state and implications of research on biological effects of millimeter waves: a review of the literature. , 1998, Bioelectromagnetics.

[10]  Francesca Apollonio,et al.  Considerations for Developing an RF Exposure System: A Review for in vitro Biological Experiments , 2010, IEEE Transactions on Microwave Theory and Techniques.

[11]  Stanislav I. Alekseev,et al.  Millimeter-Wave Absorption by Cutaneous Blood Vessels: A Computational Study , 2009, IEEE Transactions on Biomedical Engineering.

[12]  Ronan Sauleau,et al.  Numerical and experimental approaches to millimeter wave dosimetry in vitro , 2008 .

[13]  Mingtuo Zhou,et al.  Millimeter Wave Technology in Wireless PAN, LAN, and MAN , 2007 .

[14]  Ronan Sauleau,et al.  Absence of direct effect of low-power millimeter-wave radiation at 60.4 GHz on endoplasmic reticulum stress , 2009, Cell Biology and Toxicology.

[15]  M C Ziskin,et al.  Millimeter wave dosimetry of human skin , 2008, Bioelectromagnetics.

[16]  Peter H. Siegel,et al.  Impact of low intensity millimetre waves on cell functions , 2010 .

[17]  Ronan Sauleau,et al.  Millimeter-wave interactions with the human body: state of knowledge and recent advances , 2011, International Journal of Microwave and Wireless Technologies.

[18]  Xianghong Arakaki,et al.  Modulation of neuronal activity and plasma membrane properties with low-power millimeter waves in organotypic cortical slices , 2010, Journal of neural engineering.

[19]  T. Walters,et al.  Heating and pain sensation produced in human skin by millimeter waves: comparison to a simple thermal model. , 2000, Health physics.

[20]  Luciano Tarricone,et al.  The response of giant phospholipid vesicles to millimeter waves radiation. , 2009, Biochimica et biophysica acta.

[21]  M C Ziskin,et al.  Reactions of keratinocytes to in vitro millimeter wave exposure , 2001, Bioelectromagnetics.

[22]  Ronan Sauleau,et al.  Study of narrow band millimeter‐wave potential interactions with endoplasmic reticulum stress sensor genes , 2009, Bioelectromagnetics.

[23]  H. Chiang,et al.  Millimeter wave exposure reverses TPA suppression of gap junction intercellular communication in HaCaT human keratinocytes , 2004, Bioelectromagnetics.

[24]  S. Alekseev,et al.  Influence of blood flow and millimeter wave exposure on skin temperature in different thermal models , 2009, Bioelectromagnetics.

[25]  M Zhadobov,et al.  Low‐power millimeter wave radiations do not alter stress‐sensitive gene expression of chaperone proteins , 2007, Bioelectromagnetics.

[26]  N. Chemeris,et al.  The role of fatty acids in anti‐inflammatory effects of low‐intensity extremely high‐frequency electromagnetic radiation , 2011, Bioelectromagnetics.

[27]  R. Sauleau,et al.  Evaluation of the Potential Biological Effects of the 60-GHz Millimeter Waves Upon Human Cells , 2009, IEEE Transactions on Antennas and Propagation.

[28]  A. Ahlbom.  Guidelines for limiting exposure to time-varying electric, magnetic, and electromagnetic fields (up to 300 GHz) , 1998 .