Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.).

A robust Agrobacterium-mediated transformation procedure was developed for Rigel, a commercial cultivar of evening primrose, and used to deliver a cDNA encoding a Delta(6)-desaturase from borage under the control of a cauliflower mosaic virus (CaMV) 35S promoter. Analysis of the transformed plants demonstrated an altered profile of polyunsaturated fatty acids, with an increase in gamma-linolenic acid and octadecatetraenoic acid in leaf tissues when compared with control lines.

[1]  K. Rathore,et al.  Developmental and tissue-specific expression of CaMV 35S promoter in cotton as revealed by GFP , 2002, Plant Molecular Biology.

[2]  K. Shimamoto,et al.  Expression of CaMV35S-GUS gene in transgenic rice plants , 1990, Molecular and General Genetics MGG.

[3]  C. Sparks,et al.  Factors influencing successful Agrobacterium-mediated genetic transformation of wheat , 2003, Plant Cell Reports.

[4]  J. Fox.  Puzzling industry response to ProdiGene fiasco , 2003, Nature Biotechnology.

[5]  P. Covello,et al.  High-Level Production of γ-Linolenic Acid in Brassica juncea Using a Δ6 Desaturase from Pythium irregulare , 2002, Plant Physiology.

[6]  P. Covello,et al.  High-level production of gamma-linolenic acid in Brassica juncea using a delta6 desaturase from Pythium irregulare. , 2002, Plant physiology.

[7]  P. Lazzeri,et al.  High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron , 2001 .

[8]  L. Chuang,et al.  Characterization of oil exhibiting high γ-linolenic acid from a genetically transformed canola strain , 2001 .

[9]  C. Sparks,et al.  Factors influencing Agrobacterium-mediated transient expression of uidA in wheat inflorescence tissue. , 2001, Journal of experimental botany.

[10]  D. Barre.  Potential of Evening Primrose, Borage, Black Currant, and Fungal Oils in Human Health , 2001, Annals of Nutrition and Metabolism.

[11]  P. Shewry,et al.  Accumulation of Δ6-unsaturated fatty acids in transgenic tobacco plants expressing a Δ6-desaturase from Borago officinalis , 1999 .

[12]  L. Kunst,et al.  Accumulation of Very-Long-Chain Fatty Acids in Membrane Glycerolipids Is Associated with Dramatic Alterations in Plant Morphology , 1998, Plant Cell.

[13]  R. Herrmann,et al.  Tissue culture of wild-type, interspecific genome/plastome hybrids and plastome mutants of evening primrose (Oenothera): controlled morphogenesis and transformation , 1998, Plant Cell Reports.

[14]  R. Herrmann,et al.  Plant regeneration from leaf protoplasts of evening primrose (Oenothera hookeri) , 1998, Plant Cell Reports.

[15]  R. Kalla,et al.  Agrobacterium tumefaciens‐mediated barley transformation , 1997 .

[16]  P. Shewry,et al.  Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6-desaturated fatty acids in transgenic tobacco. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[17]  T. Thomas,et al.  Expression of a cyanobacterial delta 6-desaturase gene results in gamma-linolenic acid production in transgenic plants. , 1996, Nature biotechnology.

[18]  J. F. Thompson,et al.  Evening primrose oil and atopic eczema , 1995, The Lancet.

[19]  P. Isaac,et al.  Isolation of DNA from plants. , 1994, Methods in molecular biology.

[20]  D. Horrobin.  Nutritional and medical importance of gamma-linolenic acid. , 1992, Progress in lipid research.

[21]  R. A. Ludwig,et al.  A DNA Transformation–Competent Arabidopsis Genomic Library in Agrobacterium , 1991, Bio/Technology.

[22]  D. Horrobin,et al.  Evening primrose oil and atopic eczema , 1990, The Lancet.

[23]  P. Benfey,et al.  Tissue‐specific expression from CaMV 35S enhancer subdomains in early stages of plant development. , 1990, The EMBO journal.

[24]  B. Forde,et al.  Efficient transformation of Agrobacterium spp. by high voltage electroporation. , 1989, Nucleic acids research.

[25]  P. Benfey,et al.  Regulated Genes in Transgenic Plants , 1989, Science.

[26]  C. Kies.  Evening primose oil: a source of gamma linolenic acid , 1989 .

[27]  I. Kiewitt,et al.  Formation of .gamma.-linolenic acid in the higher plant evening primrose (Oenothera biennis L.) , 1987 .

[28]  M. Bevan,et al.  Binary Agrobacterium vectors for plant transformation. , 1984, Nucleic acids research.

[29]  B. Hudson.  Evening primrose (Oenothera spp.) oil and seed , 1984 .

[30]  F. Skoog,et al.  A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .

[31]  F. Skoog,et al.  A revised medium for the growth and bioassay with tobacco tissue culture , 1962 .

[32]  W. J. Dyer,et al.  A rapid method of total lipid extraction and purification. , 1959, Canadian journal of biochemistry and physiology.