Increasing the thermostability of sucrose phosphorylase by a combination of sequence- and structure-based mutagenesis.
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Wim Soetaert | Remko Kuipers | Henk-Jan Joosten | An Cerdobbel | Remko K. P. Kuipers | Tom Desmet | W. Soetaert | H. Joosten | T. Desmet | Dirk Aerts | Karel De Winter | Dirk Aerts | Karel De Winter | A. Cerdobbel
[1] Uwe T Bornscheuer,et al. Thermostabilization of an esterase by alignment-guided focussed directed evolution. , 2010, Protein engineering, design & selection : PEDS.
[2] Manfred T Reetz,et al. Addressing the Numbers Problem in Directed Evolution , 2008, Chembiochem : a European journal of chemical biology.
[3] W. Soetaert,et al. Sucrose phosphorylase as cross-linked enzyme aggregate: improved thermal stability for industrial applications. , 2010, Biotechnology journal.
[4] 隆 栗木,et al. ランダム変異を用いたStreptococcus mutans 由来 Sucrose Phosphorylaseの耐熱性の向上 , 2006 .
[5] P. Kollman,et al. How well does a restrained electrostatic potential (RESP) model perform in calculating conformational energies of organic and biological molecules? , 2000 .
[6] Bernard Henrissat,et al. Dividing the large glycoside hydrolase family 13 into subfamilies: towards improved functional annotations of alpha-amylase-related proteins. , 2006, Protein engineering, design & selection : PEDS.
[7] J. Kastrup,et al. Crystal structure of sucrose phosphorylase from Bifidobacterium adolescentis. , 2004, Biochemistry.
[8] R. Silverstein,et al. Purification and mechanism of action of sucrose phosphorylase. , 1967, The Journal of biological chemistry.
[9] M. Lehmann,et al. The consensus concept for thermostability engineering of proteins. , 2000, Biochimica et biophysica acta.
[10] Frances H. Arnold,et al. In the Light of Evolution III: Two Centuries of Darwin Sackler Colloquium: In the light of directed evolution: Pathways of adaptive protein evolution , 2009 .
[11] B. Nidetzky,et al. A high-yielding biocatalytic process for the production of 2-O-(alpha-D-glucopyranosyl)-sn-glycerol, a natural osmolyte and useful moisturizing ingredient. , 2008, Angewandte Chemie.
[12] Andreas Vogel,et al. Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability. , 2006, Angewandte Chemie.
[13] Dan S. Tawfik,et al. Chaperonin overexpression promotes genetic variation and enzyme evolution , 2009, Nature.
[14] D. Cowan,et al. Thermophilic proteins: stability and function in aqueous and organic solvents. , 1997, Comparative biochemistry and physiology. Part A, Physiology.
[15] V. Eijsink,et al. Rational engineering of enzyme stability. , 2004, Journal of biotechnology.
[16] Dan S. Tawfik,et al. Stability effects of mutations and protein evolvability. , 2009, Current opinion in structural biology.
[17] Jiro Shimada,et al. Rational Design of Thermostable Lactate Oxidase by Analyzing Quaternary Structure and Prevention of Deamidation , 2005, Biotechnology Letters.
[18] B. Matthews,et al. Enhanced protein thermostability from designed mutations that interact with α-helix dipoles , 1990, Nature.
[19] O. H. Lowry,et al. Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.
[20] D. Haltrich,et al. alpha-1,4-D-glucan phosphorylase of gram-positive Corynebacterium callunae: isolation, biochemical properties and molecular shape of the enzyme from solution X-ray scattering. , 1997, The Biochemical journal.
[21] B. Nidetzky,et al. Mechanistic differences among retaining disaccharide phosphorylases: insights from kinetic analysis of active site mutants of sucrose phosphorylase and alpha,alpha-trehalose phosphorylase. , 2008, Carbohydrate research.
[22] G. Haki,et al. Developments in industrially important thermostable enzymes: a review. , 2003, Bioresource technology.
[23] M. De Mey,et al. A constitutive expression system for high‐throughput screening , 2011 .
[24] B. Matthews,et al. Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding. , 1987, Proceedings of the National Academy of Sciences of the United States of America.
[25] Kuriki Takashi,et al. ランダム変異を用いたStreptococcus mutans由来Sucrose Phosphorylaseの耐熱性の向上 , 2006 .
[26] W. Soetaert,et al. Increasing the thermostability of sucrose phosphorylase by multipoint covalent immobilization. , 2010, Journal of biotechnology.
[27] J. Chaparro-Riggers,et al. Thermostable variants constructed via the structure-guided consensus method also show increased stability in salts solutions and homogeneous aqueous-organic media. , 2008, Protein engineering, design & selection : PEDS.
[28] Gert Vriend,et al. Increasing the precision of comparative models with YASARA NOVA—a self‐parameterizing force field , 2002, Proteins.
[29] A. Fersht,et al. Estimating the contribution of engineered surface electrostatic interactions to protein stability by using double-mutant cycles. , 1990, Biochemistry.
[30] M. Lehmann,et al. Engineering proteins for thermostability: the use of sequence alignments versus rational design and directed evolution. , 2001, Current opinion in biotechnology.
[31] Gert Vriend,et al. 3DM: Systematic analysis of heterogeneous superfamily data to discover protein functionalities , 2010, Proteins.
[32] B. Nidetzky,et al. Sucrose phosphorylase: a powerful transglucosylation catalyst for synthesis of α-D-glucosides as industrial fine chemicals , 2010 .