In Vitro Genotoxicity of Polystyrene Nanoparticles on the Human Fibroblast Hs27 Cell Line

Several studies have provided information on environmental nanoplastic particles/debris, but the in vitro cyto-genotoxicity is still insufficiently characterized. The aim of this study is to analyze the effects of polystyrene nanoparticles (PNPs) in the Hs27 cell line. The viability of Hs27 cells was determined following exposure at different time windows and PNP concentrations. The genotoxic effects of the PNPs were evaluated by the cytokinesis-block micronucleus (CBMN) assay after exposure to PNPs. We performed ROS analysis on HS27 cells to detect reactive oxygen species at different times and treatments in the presence of PNPs alone and PNPs added to the Crocus sativus L. extract. The different parameters of the CBMN test showed DNA damage, resulting in the increased formation of micronuclei and nuclear buds. We noted a greater increase in ROS production in the short treatment times, in contrast, PNPs added to Crocus sativus extract showed the ability to reduce ROS production. Finally, the SEM-EDX analysis showed a three-dimensional structure of the PNPs with an elemental composition given by C and O. This work defines PNP toxicity resulting in DNA damage and underlines the emerging problem of polystyrene nanoparticles, which extends transversely from the environment to humans; further studies are needed to clarify the internalization process.

[1]  N. Chandrasekaran,et al.  Distinctive impact of polystyrene nano-spherules as an emergent pollutant toward the environment , 2018, Environmental Science and Pollution Research.

[2]  S. Carradori,et al.  Crocus sativus L. stigmas and byproducts: Qualitative fingerprint, antioxidant potentials and enzyme inhibitory activities. , 2018, Food research international.

[3]  F. Gauffre,et al.  Current opinion: What is a nanoplastic? , 2018, Environmental pollution.

[4]  M. Dhar,et al.  A comprehensive review of the pharmacological potential of Crocus sativus and its bioactive apocarotenoids. , 2018, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[5]  Jeffrey Farner Budarz,et al.  Microplastics and Nanoplastics in Aquatic Environments: Aggregation, Deposition, and Enhanced Contaminant Transport. , 2017, Environmental science & technology.

[6]  Al-Khedhairy.  Cellular and Molecular Toxicology of Nanoparticles , 2018, Advances in Experimental Medicine and Biology.

[7]  Isa Doverbratt,et al.  Nanoplastics in the aquatic environment , 2018 .

[8]  A. ter Halle,et al.  Nanoplastic in the North Atlantic Subtropical Gyre. , 2017, Environmental science & technology.

[9]  C. Delporte,et al.  The waste of saffron crop, a cheap source of bioactive compounds , 2017 .

[10]  N. Tufenkji,et al.  Are There Nanoplastics in Your Personal Care Products , 2017 .

[11]  F. Kelly,et al.  Plastic and Human Health: A Micro Issue? , 2017, Environmental science & technology.

[12]  E. Lahive,et al.  Microplastics in freshwater and terrestrial environments: Evaluating the current understanding to identify the knowledge gaps and future research priorities. , 2017, The Science of the total environment.

[13]  J. Lourenço,et al.  Histopathological and molecular effects of microplastics in Eisenia andrei Bouché. , 2017, Environmental pollution.

[14]  K. Wyles,et al.  Microplastics in personal care products : Exploring perceptions of environmentalists , beauticians and students , 2016 .

[15]  Hongyan Guo,et al.  Effects of nanoplastics and microplastics on toxicity, bioaccumulation, and environmental fate of phenanthrene in fresh water. , 2016, Environmental pollution.

[16]  M. Wagner,et al.  Formation of microscopic particles during the degradation of different polymers. , 2016, Chemosphere.

[17]  Jiana Li,et al.  Microplastics in mussels along the coastal waters of China. , 2016, Environmental pollution.

[18]  T. Romeo,et al.  Intestinal alterations in European sea bass Dicentrarchus labrax (Linnaeus, 1758) exposed to microplastics: Preliminary results. , 2016, Environmental pollution.

[19]  F. Lagarde,et al.  Is there any consistency between the microplastics found in the field and those used in laboratory experiments? , 2016, Environmental pollution.

[20]  Johan Robbens,et al.  Oyster reproduction is affected by exposure to polystyrene microplastics , 2016, Proceedings of the National Academy of Sciences.

[21]  T. Galloway,et al.  Ingestion of Nanoplastics and Microplastics by Pacific Oyster Larvae. , 2015, Environmental science & technology.

[22]  B. D. Hardesty,et al.  Threat of plastic pollution to seabirds is global, pervasive, and increasing , 2015, Proceedings of the National Academy of Sciences.

[23]  Hans Bouwmeester,et al.  Potential Health Impact of Environmentally Released Micro- and Nanoplastics in the Human Food Production Chain: Experiences from Nanotoxicology. , 2015, Environmental science & technology.

[24]  C. Wilcox,et al.  Plastic waste inputs from land into the ocean , 2015, Science.

[25]  Hans Bouwmeester,et al.  In vitro gastrointestinal digestion increases the translocation of polystyrene nanoparticles in an in vitro intestinal co-culture model , 2015, Nanotoxicology.

[26]  Elaine S. Fileman,et al.  The impact of polystyrene microplastics on feeding, function and fecundity in the marine copepod Calanus helgolandicus. , 2015, Environmental science & technology.

[27]  K. Dawson,et al.  Accumulation and embryotoxicity of polystyrene nanoparticles at early stage of development of sea urchin embryos Paracentrotus lividus. , 2014, Environmental science & technology.

[28]  Carlos M. Duarte,et al.  Plastic debris in the open ocean , 2014, Proceedings of the National Academy of Sciences.

[29]  P. Morrison,et al.  Assimilation of polybrominated diphenyl ethers from microplastics by the marine amphipod, Allorchestes compressa. , 2014, Environmental science & technology.

[30]  E. Foekema,et al.  Leaching of plastic additives to marine organisms. , 2014, Environmental pollution.

[31]  Richard C. Thompson,et al.  Microplastic Moves Pollutants and Additives to Worms, Reducing Functions Linked to Health and Biodiversity , 2013, Current Biology.

[32]  Si-Shen Feng,et al.  Effects of Particle Size and Surface Modification on Cellular Uptake and Biodistribution of Polymeric Nanoparticles for Drug Delivery , 2013, Pharmaceutical Research.

[33]  A A Koelmans,et al.  Effects of nanopolystyrene on the feeding behavior of the blue mussel (Mytilus edulis L.) , 2012, Environmental toxicology and chemistry.

[34]  L. Hansson,et al.  Food Chain Transport of Nanoparticles Affects Behaviour and Fat Metabolism in Fish , 2012, PloS one.

[35]  Albert A Koelmans,et al.  Potential scenarios for nanomaterial release and subsequent alteration in the environment , 2012, Environmental toxicology and chemistry.

[36]  Hong Xie,et al.  Genotoxicity of metal nanoparticles , 2011, Reviews on environmental health.

[37]  Anthony L Andrady,et al.  Microplastics in the marine environment. , 2011, Marine pollution bulletin.

[38]  P. Bhattacharya,et al.  Physical Adsorption of Charged Plastic Nanoparticles Affects Algal Photosynthesis , 2010 .

[39]  H. Karlsson,et al.  The comet assay in nanotoxicology research , 2010, Analytical and bioanalytical chemistry.

[40]  Francesco Stellacci,et al.  Effect of surface properties on nanoparticle-cell interactions. , 2010, Small.

[41]  J. Evan Ward,et al.  Marine aggregates facilitate ingestion of nanoparticles by suspension-feeding bivalves. , 2009, Marine environmental research.

[42]  M. Gregory.  Environmental implications of plastic debris in marine settings—entanglement, ingestion, smothering, hangers-on, hitch-hiking and alien invasions , 2009, Philosophical Transactions of the Royal Society B: Biological Sciences.

[43]  Anthony L Andrady,et al.  Applications and societal benefits of plastics , 2009, Philosophical Transactions of the Royal Society B: Biological Sciences.

[44]  N. Mrosovsky,et al.  Leatherback turtles: the menace of plastic. , 2009, Marine pollution bulletin.

[45]  S. Doak,et al.  NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials. , 2009, Biomaterials.

[46]  M. Fenech.  Cytokinesis-block micronucleus cytome assay , 2007, Nature Protocols.

[47]  Mark R Wiesner,et al.  Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. , 2006, Nano letters.

[48]  Un Environment Evaluation Office.  Evaluation of the UNEP Division of Early Warning and Assessment , 2006 .

[49]  Alexander T. Florence,et al.  Enhanced Oral Uptake of Tomato Lectin-Conjugated Nanoparticles in the Rat , 1997, Pharmaceutical Research.

[50]  B. Macdonald,et al.  Postingestive selection in the sea scallop (Placopectenmagellanicus) on the basis of chemical properties of particles , 2002 .

[51]  D. W. Laist.  Impacts of Marine Debris: Entanglement of Marine Life in Marine Debris Including a Comprehensive List of Species with Entanglement and Ingestion Records , 1997 .

[52]  E. Frankel.  Nutritional Benefits of Flavonoids , 1997 .

[53]  M Rabinovitch,et al.  Professional and non-professional phagocytes: an introduction. , 1995, Trends in cell biology.

[54]  J. G. Cory,et al.  Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. , 1991, Cancer communications.

[55]  R Harkov,et al.  Health effects. , 1980, Journal of the Air Pollution Control Association.