Individual housing of female mice : influence on post-surgical behaviour and recovery Short title : Individual housing of mice after surgery

Individual housing of laboratory mice may increase vulnerability to surgical stress, and interfere with postsurgical recovery. To analyse the effect of housing conditions on recovery, pairand single-housed female C57BL/6J mice underwent a minor laparotomy +/analgesia, anaesthesia only or no treatment. Animals were monitored using non-invasive methods during the immediate postsurgical period to assess pain and general impairment. While no appearance or posture abnormalities were observed postexperiment, home cage behaviours were affected distinctly. Discriminant analysis identified self-grooming, locomotion, climbing and resting as mainly responsible for experimental group separation. Behavioural rhythmicity was disrupted, and behaviours related to wellbeing, such as nest building, climbing and burrowing, decreased. Behavioural pain signs (e.g. press) increased. Most behavioural alterations showed a gradation between treatments, e.g. burrowing latency ranged from an intermediate level following anaesthesia only and surgery with analgesia, to pronounced prolongation after surgery without analgesia. Significantly lower burrowing performance after surgery without analgesia in individuallyhoused animals indicates better recovery in pairs. Social interaction in pairs–an important component of normal behaviour (64%) and a potential indicator for direct social support–was nearly absent (0.3-0.5%). While anaesthesia and surgery resulted in clear changes in behaviour, differences between housing conditions were minor. Hence, despite a tendency towards better recovery in pairs, we found no distinct negative effect of individual housing. In conclusion, both housing conditions are acceptable during the period immediately following minor surgery, though social housing is always preferable in female mice. DOI: https://doi.org/10.1258/la.2012.012027 Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-69188 Originally published at: Jirkof, Paulin; Cesarovic, Nikola; Rettich, Andreas; Fleischmann, Thea; Arras, Margarete (2012). Individual housing of female mice: influence on postsurgical behaviour and recovery. Laboratory Animals, 46(4):325-334. DOI: https://doi.org/10.1258/la.2012.012027

[1]  Nikola Cesarovic,et al.  Implantation of Radiotelemetry Transmitters Yielding Data on ECG, Heart Rate, Core Body Temperature and Activity in Free-moving Laboratory Mice , 2011, Journal of visualized experiments : JoVE.

[2]  B. Seifert,et al.  Burrowing Behavior as an Indicator of Post-Laparotomy Pain in Mice , 2010, Front. Behav. Neurosci..

[3]  G. Norman,et al.  Social Interaction Prevents the Development of Depressive-Like Behavior Post Nerve Injury in Mice: A Potential Role for Oxytocin , 2010, Psychosomatic medicine.

[4]  B. Hagman,et al.  Housing environment influences the need for pain relief during post-operative recovery in mice , 2010, Physiology & Behavior.

[5]  S. Parmigiani,et al.  Effects of Housing Social Context on Emotional Behaviour and Physiological Responses in Female Mice , 2009 .

[6]  P. Flecknell,et al.  The analgesic effects of oral paracetamol in two strains of mice undergoing vasectomy , 2009, Laboratory animals.

[7]  Sue-Hyun Lee,et al.  Social Isolation Selectively Increases Anxiety in Mice without Affecting Depression-like Behavior. , 2009, The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology.

[8]  F. J. van der Staay,et al.  Individual housing of mice — Impact on behaviour and stress responses , 2009, Physiology & Behavior.

[9]  G. Norman,et al.  Social isolation alters neuroinflammatory response to stroke , 2009, Proceedings of the National Academy of Sciences.

[10]  V. Baumans,et al.  Impact of 'living apart together' on postoperative recovery of mice compared with social and individual housing , 2007, Laboratory animals.

[11]  M. Arras,et al.  Assessment of post-laparotomy pain in laboratory mice by telemetric recording of heart rate and heart rate variability , 2007, BMC veterinary research.

[12]  J. Roughan,et al.  Effects of vasectomy surgery and meloxicam treatment on faecal corticosterone levels and behaviour in two strains of laboratory mouse , 2007, PAIN.

[13]  I. Olsson,et al.  More than numbers matter: the effect of social factors on behaviour and welfare of laboratory rodents and non-human primates , 2007 .

[14]  R. Deacon.  Assessing nest building in mice , 2006, Nature Protocols.

[15]  H. Rauvala,et al.  Long‐term individual housing in C57BL/6J and DBA/2 mice: assessment of behavioral consequences , 2004, Genes, brain, and behavior.

[16]  V. Baumans,et al.  Preference for social contact versus environmental enrichment in male laboratory mice , 2004, Laboratory animals.

[17]  P. Sacerdote,et al.  Individual housing induces altered immuno-endocrine responses to psychological stress in male mice , 2003, Psychoneuroendocrinology.

[18]  T. Rülicke,et al.  Higher heart rate of laboratory mice housed individually vs in pairs , 2003, Laboratory animals.

[19]  N. Lavidis,et al.  Stressful animal housing conditions and their potential effect on sympathetic neurotransmission in mice. , 2002, American journal of physiology. Regulatory, integrative and comparative physiology.

[20]  P. Rehbinder,et al.  Recommendations for the health monitoring of rodent and rabbit colonies in breeding and experimental units , 2002, Laboratory animals.

[21]  J. Roughan,et al.  Behavioural effects of laparotomy and analgesic effects of ketoprofen and carprofen in rats , 2001, Pain.

[22]  S. Parmigiani,et al.  Interindividual Variability in Swiss Male Mice: Relationship between Social Factors, Aggression, and Anxiety , 1998, Physiology & Behavior.

[23]  R. Bauman,et al.  Effects of chronic stress on sleep in rats , 1995, Physiology & Behavior.

[24]  G. A. Oortmerssen.  Biological significance, genetics and evolutionary origin of variability in behaviour within and between inbred strains of mice Mus musculus , 1971 .

[25]  S. Selvarasu,et al.  Open Access Research , 2022 .