The Humoral Response to DNCB: Its Detection by Different Techniques 1

Summary Antibodies in the sera of guinea pigs immunized with DNCB were detected by Ouchterlony-type analyses and were estimated in weight units by the mABC procedure. Assays of these sera by means of passive cutaneous anaphylaxis, passive he-molysis, or the ABC-33 antigen-binding technique were essentially negative. The validity of the mABC analyses was established by comparison of results obtained in quantitative precipitation studies with sera of guinea pigs immunized with DNP · BGG. In these instances, also, the ABC-33 assays failed to detect all the anti-DNP immuno-globulins.