Development of a fast-scanning combined ultrasound-photoacoustic biomicroscope

Recently a realtime photoacoustic microscopy system has been demonstrated. Unfortunately, however, displayed B-scan images were sometimes difficult to interpret as there was little structural context. In this work, we provide structural context for photoacoustic microscopy images by adding ultrasound biomicroscopy as a complementary and synergistic modality. Our system uses a voice-coil translation stage capable of 1" lateral translation, and can operate in excess of 15 Hz for 1-cm translations, providing up to 30 ultrasound frames per second. The frame-rate of the photoacoustic acquisitions is limited by the 20-Hz pulse-repetition rate of the laser, but can be increased with a faster-repetition-rate laser. Data from the system is streamed in real time from a 2GS/s PCI data acquisition card to the host PC at rates as high as 200 MB/s. The system should prove useful for various in vivo studies, including combined ultrasound Doppler and photoacoustic imaging.

[1]  Lihong V. Wang,et al.  In vivo dark-field reflection-mode photoacoustic microscopy. , 2005, Optics letters.

[2]  Lihong V. Wang,et al.  Optical-resolution photoacoustic microscopy for in vivo imaging of single capillaries. , 2008, Optics letters.

[3]  Hao F. Zhang Functional Photoacoustic Microscopy , 2009 .

[4]  Geng Ku,et al.  Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography. , 2006, Journal of biomedical optics.

[5]  E. Chérin,et al.  A new ultrasound instrument for in vivo microimaging of mice. , 2002, Ultrasound in medicine & biology.

[6]  Liang Song,et al.  Realtime photoacoustic microscopy in vivo with a 30-MHz ultrasound array transducer. , 2008, Optics express.

[7]  Lihong V. Wang,et al.  Limitations of quantitative photoacoustic measurements of blood oxygenation in small vessels , 2007, Physics in medicine and biology.

[8]  F. Stuart Foster,et al.  High-frequency color flow imaging of the microcirculation , 2000 .

[9]  A. Needles,et al.  Inter-frame clutter filtering for high frequency flow imaging , 2004, IEEE Ultrasonics Symposium, 2004.

[10]  Wei Wang,et al.  Simultaneous Molecular and Hypoxia Imaging of Brain Tumors In Vivo Using Spectroscopic Photoacoustic Tomography , 2008, Proceedings of the IEEE.

[11]  Lihong V. Wang,et al.  Photoacoustic imaging of the microvasculature with a high-frequency ultrasound array transducer. , 2007, Journal of biomedical optics.

[12]  K.K. Shung,et al.  Development of a 35-MHz piezo-composite ultrasound array for medical imaging , 2006, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[13]  L.H. Wang,et al.  1E-1 Photoacoustic Microscopy with a 30MHz Array and Receive System , 2006, 2006 IEEE Ultrasonics Symposium.

[14]  David E Goertz,et al.  High-frequency 3-D color-flow imaging of the microcirculation. , 2003, Ultrasound in medicine & biology.

[15]  Lihong V. Wang,et al.  Photoacoustic imaging of lacZ gene expression in vivo. , 2007, Journal of biomedical optics.