Decarboxylase activity of Serratia marcescens depending on pH and chosen monosaccharide content

The effect of pH (6, 7 and 8) and glucose, galactose and fructose content (0.00, 0.25, 0.50, 0.75 and 1.00% w/v) on growth and decarboxylase activity of Serratia marcescens was evaluated. Nutrient broth with 0.2% w/v of ornithine was used for the cultivation of bacteria. Biogenic amine production was determined using the ion-exchange chromatography with post-column ninhydrine derivatization and spectrophotometric detection. While the optimal pH for bacteria growth was 7–8, the decarboxylases showed maximal activity at pH 6. Monosaccharides stimulated both bacteria growth and putrescine formation at pH 6. On the contrary, addition of all monosaccharides tested caused inhibition of putrescine production at pH 7 and 8. Key-Words: Serratia marcescens, pH, monosaccharides, decarboxylase activity, biogenic amines, ornithine, putrescine

[1]  L. Buňková,et al.  Formation of biogenic amines by Gram-negative bacteria isolated from poultry skin , 2010 .

[2]  L. Buňková,et al.  Tyramine production of technological important strains of Lactobacillus, Lactococcus and Streptococcus , 2009 .

[3]  Armağan Önal,et al.  A review: Current analytical methods for the determination of biogenic amines in foods , 2007 .

[4]  N. Innocente,et al.  Determination of biogenic amines in cheese using HPLC technique and direct derivatization of acid extract , 2007 .

[5]  J. Karovičová,et al.  Effects of NaCl concentration and initial pH value on biogenic amine formation dynamics by Enterobacter spp. bacteria in model conditions , 2006 .

[6]  G. Greif.  Effects of NaCl concentration and initial pH value on biogenic amine formation dynamics by Enterobacter spp. bacteria in model conditions , 2006 .

[7]  Tom A. McMeekin,et al.  Handbook of Food Science, Technology and Engineering , 2006 .

[8]  P. Kalač,et al.  A review of dietary polyamines: Formation, implications for growth and health and occurrence in foods , 2005 .

[9]  M. H. Silla-Santos.  Toxic Nitrogen Compounds Produced during Processing: Biogenic Amines, Ethyl Carbamides, Nitrosamines , 2004 .

[10]  G. Suzzi,et al.  Effects of pH, temperature and NaCl concentration on the growth kinetics, proteolytic activity and biogenic amine production of Enterococcus faecalis. , 2001, International journal of food microbiology.

[11]  S. Bover-Cid,et al.  Improved screening procedure for biogenic amine production by lactic acid bacteria. , 1999, International journal of food microbiology.

[12]  A. Bouchereau,et al.  Polyamines and environmental challenges : recent development , 1999 .

[13]  J. Karovičová,et al.  Study of the growth and production of biogenic amines by some microorganisms in model conditions , 1999 .

[14]  A. Shalaby.  Significance of biogenic amines to food safety and human health , 1996 .

[15]  M. H. Silla Santos.  Biogenic amines: their importance in foods. , 1996, International journal of food microbiology.

[16]  W. Holzapfel,et al.  Biogenic amines and their production by microorganisms in food , 1994 .

[17]  S. Bunčić,et al.  A study of factors influencing histamine production in meat , 1994 .

[18]  M. Nout.  Fermented foods and food safety. , 1994 .