Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective.

We present a miniature, flexible two-photon microscope consisting of a fused coherent optical fiber bundle with 30,000 cores and a gradient-index lens objective. The laser focus of a standard two-photon laser-scanning microscope was scanned over the entrance surface of the fiber bundle, resulting in sequential coupling into individual cores. Fluorescent light was detected through the fiber bundle. Micrometer-sized fluorescent beads and pollen grains were readily resolved. In addition, fluorescently labeled blood vessels were imaged through the fiber bundle in rat brain in vivo.

[1]  Fritjof Helmchen,et al.  Distortion-free delivery of nanojoule femtosecond pulses from a Ti:sapphire laser through a hollow-core photonic crystal fiber. , 2004, Optics letters.

[2]  Raymond P. Molloy,et al.  In vivo multiphoton microscopy of deep brain tissue. , 2004, Journal of neurophysiology.

[3]  Reverse propagation of femtosecond pulses in optical fibers. , 2003, Optics letters.

[4]  Min Gu,et al.  Two-photon fluorescence endoscopy with a micro-optic scanning head. , 2003, Optics letters.

[5]  W. Denk,et al.  Two-photon imaging to a depth of 1000 microm in living brains by use of a Ti:Al2O3 regenerative amplifier. , 2003, Optics letters.

[6]  A. Mehta,et al.  Multiphoton endoscopy: optical design and application to in vivo imaging of mammalian hippocampal neurons , 2003, Conference on Lasers and Electro-Optics, 2003. CLEO '03..

[7]  Fritjof Helmchen,et al.  Miniaturization of Fluorescence Microscopes Using Fibre Optics , 2002, Experimental physiology.

[8]  A. Mazzolini,et al.  Optic fibre bundle contact imaging probe employing a laser scanning confocal microscope , 2002, Journal of microscopy.

[9]  D. Tank,et al.  Enhanced two-photon excitation through optical fiber by single-mode propagation in a large core. , 2002, Applied optics.

[10]  D. Tank,et al.  A Miniature Head-Mounted Two-Photon Microscope High-Resolution Brain Imaging in Freely Moving Animals , 2001, Neuron.

[11]  F. Wise,et al.  Fiber delivery of femtosecond pulses from a Ti:sapphire laser. , 2001, Optics letters.

[12]  B Messerschmidt,et al.  Endoscope-compatible confocal microscope using a gradient index-lens system , 2001 .

[13]  Y. Sabharwal,et al.  Slit-scanning confocal microendoscope for high-resolution in vivo imaging. , 1999, Applied optics.

[14]  Sunanda Mitra,et al.  Method for depixelating micro-endoscopic images , 1999 .

[15]  K. Svoboda,et al.  Photon Upmanship: Why Multiphoton Imaging Is More than a Gimmick , 1997, Neuron.