Coupling of Inositol Phospholipid Metabolism with Excitatory Amino Acid Recognition Sites in Rat Hippocampus

Abstract: Ibotenate, a rigid structural analogue of glutamate, markedly enhances the hydrolysis of membrane inositol phospholipids, as reflected by the stimulation of [3H]inositol monophosphate formation in rat hippocampal slices prelabeled with [3H]inositol and treated with Li+. Quisqualate, homocysteate, l‐glutamate, and l‐aspartate also induce a significant (albeit weaker) increase in [3H]inositol monophosphate formation, whereas N‐methyl‐d‐aspartate, kainate, quinolinate, and N‐acetylaspartylglutamate are inactive. The increase in [3H]inositol monophosphate formation elicited by the above‐mentioned excitatory amino acids is potently and selectively antagonized by dl‐2‐amino‐4‐phosphonobutyric acid, a dicarboxylic amino acid receptor antagonist. These results suggest that, in the hippocampus, a class of dicarboxylic amino acid recognition sites is coupled with phospholipase C, the enzyme that catalyzes the hydrolysis of membrane inositol phospholipids.

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