Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae

The GAL1 and GAL10 genes of Saccharomyces cerevisiae are divergently transcribed, with 606 base pairs of DNA separating their transcription initiation sites. These two genes are stringently coregulated: their expression is induced ca. 1,000-fold in cells growing on galactose and is repressed by growth on glucose. The nucleotide sequence of the region of DNA between these genes and the precise sites of transcription initiation are presented here. The most notable feature of the nucleotide sequence of this region is a 108-base-pair guanine-plus-cytosine-rich stretch of DNA located approximately in the middle of the region between GAL1 and GAL10. Analysis of the effects of mutations that alter the region between these two genes, constructed in vitro or selected in vivo, suggest that these guanine-plus-cytosine-rich sequences are required for the expression of both genes. The region of DNA between GAL1 and GAL10 is sufficient for regulation of expression of these genes: fusion of the region to the yeast HIS3 gene places HIS3 under GAL control.

[1]  K. Matsumoto,et al.  [Regulatory circuits for gene expression: the metabolism of galactose and phosphate in Saccharomyces cerevisiae]. , 1984, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme.

[2]  R. W. Davis,et al.  RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[3]  G. Fink,et al.  Repeated DNA sequences upstream from HIS1 also occur at several other co-regulated genes in Saccharomyces cerevisiae. , 1983, The Journal of biological chemistry.

[4]  L. Guarente,et al.  A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[5]  A. Laughon,et al.  Isolation and preliminary characterization of the GAL4 gene, a positive regulator of transcription in yeast. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[6]  H. Pelham.  A regulatory upstream promoter element in the Drosophila Hsp 70 heat-shock gene , 1982, Cell.

[7]  S. McKnight,et al.  Transcriptional control signals of a eukaryotic protein-coding gene. , 1982, Science.

[8]  G. Fink,et al.  The nucleotide sequence of the HIS4 region of yeast. , 1982, Gene.

[9]  R. W. Davis,et al.  The organization and transcription of the galactose gene cluster of Saccharomyces. , 1981, Journal of molecular biology.

[10]  R. W. Davis,et al.  Deletion analysis of the Saccharomyces GAL gene cluster. Transcription from three promoters. , 1981, Journal of molecular biology.

[11]  K. Struhl.  Deletion mapping a eukaryotic promoter. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[12]  G. Faye,et al.  Deletion mapping of sequences essential for in vivo transcription of the iso-1-cytochrome c gene. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[13]  Pierre Chambon,et al.  In vivo sequence requirements of the SV40 early promoter region , 1981, Nature.

[14]  D. Botstein,et al.  Advanced Bacterial Genetics: A Manual for Genetic Engineering , 1980 .

[15]  K. Matsumoto,et al.  Function of positive regulatory gene gal4 in the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae: evidence that the GAL81 region codes for part of the gal4 protein , 1980, Journal of bacteriology.

[16]  S. Weissman,et al.  Determination of RNA sequences by primer directed synthesis and sequencing of their cDNA transcripts. , 1980, Methods in enzymology.

[17]  P. Sharp,et al.  Transcription maps of adenovirus. , 1980, Methods in enzymology.

[18]  R. W. Davis,et al.  Replacement of chromosome segments with altered DNA sequences constructed in vitro. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[19]  P. Chambon,et al.  Organisation and sequences at the 5′ end of a cloned complete ovalbumin gene , 1979, Nature.

[20]  R. W. Davis,et al.  High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[21]  Ronald W. Davis,et al.  Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridization , 1979, Cell.

[22]  J. Hopper,et al.  Constitutive synthesis of the GAL4 protein, a galactose pathway regulator in saccharomyces cerevisiae , 1979, Cell.

[23]  G. Fink,et al.  Methods in yeast genetics , 1979 .

[24]  J. Sutcliffe.  Complete nucleotide sequence of the Escherichia coli plasmid pBR322. , 1979, Cold Spring Harbor symposia on quantitative biology.

[25]  K. Matsumoto,et al.  Genetic control of galactokinase synthesis in Saccharomyces cerevisiae: evidence for constitutive expression of the positive regulatory gene gal4 , 1978, Journal of bacteriology.

[26]  G. Fink,et al.  Transformation of yeast. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[27]  A. Singh.  Nonsense suppressors of yeast cause osmotic-sensitive growth. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[28]  R. Legerski,et al.  Extracellular nucleases of Pseudomonas BAL 31. I. Characterization of single strand-specific deoxyriboendonuclease and double-strand deoxyriboexonuclease activities. , 1975, Nucleic acids research.

[29]  B. G. Adams.  Induction of Galactokinase in Saccharomyces cerevisiae: Kinetics of Induction and Glucose Effects , 1972, Journal of bacteriology.

[30]  D. Hawthorne,et al.  Uninducible Mutants in the gal i Locus of Saccharomyces cerevisiae , 1972, Journal of bacteriology.

[31]  J. Bassel,et al.  Genetic Order of the Galactose Structural Genes in Saccharomyces cerevisiae , 1971, Journal of bacteriology.

[32]  D. Hawthorne,et al.  Regulation of genes controlling synthesis of the galactose pathway enzymes in yeast. , 1966, Genetics.

[33]  D. Hawthorne,et al.  ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES. , 1964, Genetics.

[34]  G. Pelroy,et al.  A gene controlling inducibility of the galactose pathway enzymes in Saccharomyces , 1963 .