EST-derived markers highlight genetic relationships among Plasmopara halstedii French races.

Twelve EST-derived markers were isolated from Plasmopara halstedii (Oomycetes), the causal agent of sunflower downy mildew. A total of 25 single nucleotide polymorphisms (SNPs) and five indels were detected by single-strand conformation polymorphism (SSCP) analysis and developed for highthroughput genotyping of 24 isolates. These markers reveal a good level of genetic diversity and were useful in detecting genotypic variability of French populations. A deficit in heterozygotes indicated that P. halstedii is a selfing species. For the first time, these markers allowed to reveal genetic relatedness among 14 races characterized over a 19-year period of P. halstedii monitoring in France.

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