Efeito dos gentipos para alphaS1-casena sobre as fraes proteicas e lipdicas do leite de cabra

A high polymorphism is found in the locus of goat αS1-casein gene and it is classified in four levels of expression, named high, medium, and low, associated with production of 3.6, 1.6, 0.6, and 0 g/L/allele, respectively. The study was conducted to investigate possible variations on milk yield and components, profile of casein, and lipolysis of fat. Forty-four goats were assigned to five distinct genotypes as two homozygous, one for high (AA) and the other for intermediate yield (EE); and three heterozygous named AE, AF, and EF for the αs1-casein. For lipolysis, milk was sampled in four aliquots which were treated soon after milking and 24 hours after cooling. Differences were observed for both casein yield and its fractions. No difference was found for other variables. The AA genotype presented the higher content of both casein (28.6g/L) and αS1-cn (22.3%). Other genotypes averaged 20.4g/L for casein content. Values of αS1-cn were 12.1% for heterozygous and 10.1 and 9.1% for both EE and EF genotype respectively. Cooling the milk for 24 hours increased the rate of lipolysis. Genotyping goats for the αS1-cn can be used as a tool for selecting animal targeting milk products with distinct profiles of proteins.

[1]  Ana Carolina de Souza,et al.  Estimativa da Freqüência do Alelo O do Gene da Alphas1-Caseína em Caprinos da Raça Saanen , 2008 .

[2]  M. Samolada,et al.  Casein fraction of bulk milks from different caprine breeds. , 2004 .

[3]  A. Rando,et al.  The goat αs1-casein gene: gene structure and promoter analysis , 2004 .

[4]  Monique Rijnkels,et al.  Multispecies Comparison of the Casein Gene Loci and Evolution of Casein Gene Family , 2002, Journal of Mammary Gland Biology and Neoplasia.

[5]  A Ferlay,et al.  A review of nutritional and physiological factors affecting goat milk lipid synthesis and lipolysis. , 2003, Journal of dairy science.

[6]  A. Rando,et al.  Molecular characterization of the goat CSN1S101 allele , 2003, Journal of Dairy Research.

[7]  L. Zwierzchowski,et al.  Effects of polymorphism at 5'-noncoding regions [promoters] of alphaS1- and alphaS2-casein genes on selected milk production traits in Polish Black-and-White cows , 2003 .

[8]  D. Sauvant,et al.  Lactation response and nitrogen, calcium, and phosphorus utilization of dairy goats differing by the genotype for alpha S1-casein in milk, and fed diets varying in crude protein concentration. , 2002, Journal of dairy science.

[9]  A. Riaublanc,et al.  Is the apocrine milk secretion process observed in the goat species rooted in the perturbation of the intracellular transport mechanism induced by defective alleles at the alpha(s1)-Cn locus? , 2002, Reproduction, nutrition, development.

[10]  J. Fauquant,et al.  Combined effect of whey protein and aS1-casein genotype on the heat stability of goat milk , 2001 .

[11]  M. Pappalardo,et al.  Identification of the goat CSN1S1F allele by means of PCR-RFLP method. , 2000, Animal genetics.

[12]  S. Clark,et al.  Alphas1-casein, milk composition and coagulation properties of goat milk , 2000 .

[13]  A. Trujillo,et al.  Analysis of major caprine milk proteins by reverse-phase high-performance liquid chromatography and electrospray ionization-mass spectrometry. , 2000, Journal of dairy science.

[14]  M. Ollivier-Bousquet,et al.  Alpha(S1)-casein is required for the efficient transport of beta- and kappa-casein from the endoplasmic reticulum to the Golgi apparatus of mammary epithelial cells. , 1999, Journal of cell science.

[15]  M. Ollivier-Bousquet,et al.  Genetic polymorphism of caseins: a tool to investigate casein micelle organization , 1999 .

[16]  B. Moioli,et al.  Detection of milk protein genetic polymorphisms in order to improve dairy traits in sheep and goats: a review , 1998 .

[17]  J. Medrano,et al.  Effects of milk protein genotypes on the variation for milk production traits of Holstein and Jersey cows in California. , 1997, Journal of dairy science.

[18]  J. Jordana,et al.  Gene frequencies of caprine αs1-casein polymorphism in Spanish goat breeds , 1996 .

[19]  E. Manfredi,et al.  Influence du locus de la caséine αs1 sur les performances laitières et les paramètres génétiques des chèvres de race Alpine , 1995, Genetics Selection Evolution.

[20]  J. M. Jandal Some factors affecting lipase activity in goat milk , 1995 .

[21]  F. Grosclaude,et al.  Polymorphism of β-casein in the Creole goat of Guadeloupe: evidence for a null allele , 1993, Genetics Selection Evolution.

[22]  P. Martin Polymorphisme génétique des lactoprotéines caprines , 1993 .

[23]  F. Grosclaude,et al.  αS1-CnD, another allele associated with a decreased synthesis rate at the caprine αS1-casein locus , 1989, Genetics Selection Evolution.

[24]  M. Mahé,et al.  A Mendelian polymorphism underlying quantitative variations of goat αs1-casein , 1987, Génétique, sélection, évolution.

[25]  H. Deeth,et al.  A convenient method for determining the extent of lipolysis in milk , 1975 .