ENZYMIC HYDROLYSIS OF A GLUCOMANNAN FROM JACK PINE (PINUS BANKSIANA LAMB.)

A crude glucomannan was isolated from jack pine holocellulose in 17% yield. Repeated delignification and copper complexing of this product provided a purified glucomannan (5% of the holocellulose) containing glucose, mannose, galactose, and xylose in a molar ratio of 10:25:1:1. This polysaccharide was hydrolyzed by a commercial hemicellulase preparation, the cleavage products being removed by continuous dialysis of the reaction mixture. The following oligosaccharides were isolated from the hydrolyzate and identified; yields are based on the glucomannan:

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