Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells

The key mechanisms responsible for achievement of full reproductive and developmental capability in mammals are the differentiation and transformation of granulosa cells (GCs) during folliculogenesis, oogenesis, and oocyte maturation. Although the role of 17 beta-estradiol (E2) in ovarian activity is widely known, its effect on proliferative capacity, gap junction connection (GJC) formation, and GCs-luteal cells transformation requires further research. Therefore, the goal of this study was to assess the real-time proliferative activity of porcine GCs in vitro in relation to connexin (Cx), luteinizing hormone receptor (LHR), follicle stimulating hormone receptor (FSHR), and aromatase (CYP19A1) expression during short-term (168 h) primary culture. The cultured GCs were exposed to acute (at 96 h of culture) and/or prolonged (between 0 and 168 h of culture) administration of 1.8 and 3.6 μM E2. The relative abundance of Cx36, Cx37, Cx40, Cx43, LHR, FSHR, and CYP19A1 mRNA was measured. We conclude that the proliferation capability of GCs in vitro is substantially associated with expression of Cxs, LHR, FSHR, and CYP19A1. Furthermore, the GC-luteal cell transformation in vitro may be significantly accompanied by the proliferative activity of GCs in pigs.

[1]  W. Hager,et al.  and s , 2019, Shallow Water Hydraulics.

[2]  Aaas News,et al.  Book Reviews , 1893, Buffalo Medical and Surgical Journal.

[3]  D. Albertini,et al.  Aging-related premature luteinization of granulosa cells is avoided by early oocyte retrieval. , 2015, The Journal of endocrinology.

[4]  S. Sułkowski,et al.  Eventual proapoptotic or anti-apoptotic impact of aberrantly expressed Cx43 and Cx26 can depend on ER-alpha overexpression in human endometrioid adenocarcinoma , 2015, Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology.

[5]  Byeong-chun Lee,et al.  A spatial model showing differences between juxtacrine and paracrine mutual oocyte-granulosa cells interactions. , 2015, Indian journal of experimental biology.

[6]  H. Piotrowska,et al.  Expression and cellular distribution of estrogen and progesterone receptors and the real-time proliferation of porcine cumulus cells , 2014, Zygote.

[7]  H. Piotrowska,et al.  Study on connexin gene and protein expression and cellular distribution in relation to real-time proliferation of porcine granulosa cells. , 2014, Journal of biological regulators and homeostatic agents.

[8]  M. Hsu,et al.  1,25-Dihydroxyvitamin D3 increases testosterone-induced 17beta-estradiol secretion and reverses testosterone-reduced connexin 43 in rat granulosa cells , 2014, Reproductive Biology and Endocrinology.

[9]  H. Piotrowska,et al.  Association between the expression of LHR, FSHR and CYP19 genes, cellular distribution of encoded proteins and proliferation of porcine granulosa cells in real-time. , 2014, Journal of biological regulators and homeostatic agents.

[10]  J. Vanselow,et al.  Increasing cell plating density mimics an early post-LH stage in cultured bovine granulosa cells , 2013, Cell and Tissue Research.

[11]  H. Piotrowska,et al.  Real-time proliferation of porcine cumulus cells is related to the protein levels and cellular distribution of Cdk4 and Cx43. , 2013, Theriogenology.

[12]  H. Piotrowska,et al.  Short-term Cultivation of Porcine Cumulus Cells Influences the Cyclin-dependent Kinase 4 (Cdk4) and Connexin 43 (Cx43) Protein Expression—A Real-time Cell Proliferation Approach , 2013, The Journal of reproduction and development.

[13]  B. Vanderhyden,et al.  Bidirectional communication between oocytes and follicle cells: ensuring oocyte developmental competence. , 2010, Canadian journal of physiology and pharmacology.

[14]  V. Bordignon,et al.  Synergistic effect of porcine follicular fluid and dibutyryl cyclic adenosine monophosphate on development of parthenogenetically activated oocytes from pre-pubertal gilts. , 2009, Reproduction in domestic animals = Zuchthygiene.

[15]  B. Vanderhyden,et al.  Oocyte-granulosa cell interactions during mouse follicular development: regulation of kit ligand expression and its role in oocyte growth , 2006, Reproductive biology and endocrinology : RB&E.

[16]  E. Sato,et al.  Cumulus-oocyte complex interactions during oocyte maturation. , 2004, International review of cytology.

[17]  T. Miyano,et al.  Interactions between the oocyte and surrounding somatic cells in follicular development: lessons from in vitro culture. , 2003, The Journal of reproduction and development.

[18]  M. Sułkowska,et al.  Comparative Evaluation of Estrogen and Progesterone Receptor Expression With Connexins 26 and 43 in Endometrial Cancer , 2002, International Journal of Gynecologic Cancer.

[19]  A. Menino,et al.  Survival of oocytes recovered from vitrified sheep ovarian tissues. , 2002, Animal reproduction science.

[20]  K. Shinohara,et al.  Effects of estrogen and progesterone on the expression of connexin-36 mRNA in the suprachiasmatic nucleus of female rats , 2001, Neuroscience Letters.

[21]  C. Sellitto,et al.  Oocyte-granulosa cell heterologous gap junctions are required for the coordination of nuclear and cytoplasmic meiotic competence. , 2000, Developmental biology.

[22]  S. Narumiya,et al.  Abortive expansion of the cumulus and impaired fertility in mice lacking the prostaglandin E receptor subtype EP(2). , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[23]  J. Richards,et al.  Hormone-induced proliferation and differentiation of granulosa cells: a coordinated balance of the cell cycle regulators cyclin D2 and p27Kip1. , 1998, Molecular endocrinology.

[24]  R. Stephenson.  A and V , 1962, The British journal of ophthalmology.