Carbon Nanotube-Incorporated Nanofibers for Immunosensor Preparation against CD36

The increased serum concentration of CD36 is significantly associated with atherosclerosis, insulin resistance, and diabetes mellitus. Currently, there is no sensor system used for the detection of CD36 in the clinical field. Therefore, there is a need to develop a sensor system for the detection of CD36. The large surface area/volume ratio and controllable surface conformation of electrospun nanofibers (ENs) make them highly attractive for immunosensor applications. In the present study, PS/MWCNT-PAMAM ENs were produced and used as an immobilization matrix of Anti-CD36. Thus, the electrochemical behavior of the developed nanocomposite-based ENs and their usage potential were investigated for immunosensor applications. First, an oxidized multiwall carbon nanotube (MWCNT-OH) was synthesized and modified with a polyamidoamine generation 3 (PAMAM G3) dendrimer. The synthesized MWCNT-PAMAM nanocomposite was mixed with polystyrene (PS) solutions at different ratios to produce bead-free, smooth, and uniform PS/MWCNT-PAMAM ENs. PS/MWCNT-PAMAM ENs were accumulated on a screen-printed carbon electrode (SPCE) using the electrospinning technique. A biofunctional surface on the PS/MWCNT-PAMAM EN-coated SPCE was created using carbodiimide chemistry by covalent immobilization of Anti-CD36. The analytic performance characteristics of the developed PS/MWCNT-PAMAM/Anti-CD36 immunosensor were determined by performing electrochemical measurements in the presence of the CD36 protein. The linear detection range was found to be from 5 to 40 ng/mL, and the limit of detection was calculated as 3.94 ng/mL for CD36. The developed PS/MWCNT-PAMAM/Anti-CD36 immunosensor also displayed high tolerance to interference substances, good repeatability, and high recovery percent (recovery%) for artificial blood serum analysis.

[1]  A. Karttunen,et al.  Bridging the Junction: Electrical Conductivity of Carbon Nanotube Networks , 2022, The Journal of Physical Chemistry C.

[2]  Dilek Odaci Demirkol,et al.  Graphene oxide incorporated polystyrene electrospun nanofibers for immunosensing of CD36 as a marker of diabetic plasma. , 2022, Bioelectrochemistry.

[3]  J. Keith,et al.  Quantifying Variations in Metal-Ligand Cooperative Binding Strength with Cyclic Voltammetry and Redox-Active Ligands. , 2022, Inorganic chemistry.

[4]  N. H. von Reitzenstein,et al.  Comparing the Morphologies and Adsorption Behavior of Electrospun Polystyrene Composite Fibers with 0D Fullerenes, 1D Multiwalled Carbon Nanotubes and 2D Graphene Oxides , 2021, Chemical Engineering Journal Advances.

[5]  A. Shah,et al.  Understanding the adsorption of 1 NLB antibody on polyaniline nanotubes as a function of zeta potential and surface charge density for detection of hepatitis C core antigen: A label-free impedimetric immunosensor , 2021 .

[6]  D. Correa,et al.  Nanofibers interfaces for biosensing: Design and applications , 2021, Sensors and Actuators Reports.

[7]  R. Eckel,et al.  Cardiovascular disease in diabetes, beyond glucose. , 2021, Cell metabolism.

[8]  J. Cabaj,et al.  Electrospun Nanofibers for Sensing and Biosensing Applications—A Review , 2021, International journal of molecular sciences.

[9]  Mangilal Agarwal,et al.  Overview of Nano-Fiber Mats Fabrication via Electrospinning and Morphology Analysis , 2021, Textiles.

[10]  D. Tang,et al.  CRISPR-Cas12a-driven MXene-PEDOT:PSS piezoresistive wireless biosensor , 2021 .

[11]  D. O. Demirkol,et al.  High generation dendrimer decorated poly-Ɛ-caprolactone/polyacrylic acid electrospun nanofibers for the design of a bioelectrochemical sensing surface , 2021 .

[12]  Katarina Goodge,et al.  A Comprehensive Review of the Covalent Immobilization of Biomolecules onto Electrospun Nanofibers , 2020, Nanomaterials.

[13]  K. Ozoemena,et al.  Onion-like carbon re-inforced electrospun polyacrylonitrile fibres for ultrasensitive electrochemical immunosensing of Vibrio cholerae toxin , 2020 .

[14]  S. Einum,et al.  On the use of the coefficient of variation to quantify and compare trait variation , 2020, Evolution letters.

[15]  J. Buriak,et al.  Redox Flow Batteries: How to Determine Electrochemical Kinetic Parameters. , 2020, ACS nano.

[16]  H. Khonakdar,et al.  Surface modification of MWCNT and its influence on properties of paraffin/MWCNT nanocomposites as phase change material , 2020, Journal of Applied Polymer Science.

[17]  H. Sereshti,et al.  Plasma‐functionalized Highly Aligned CNT‐based Biosensor for Point of Care Determination of Glucose in Human Blood Plasma , 2020, Electroanalysis.

[18]  D. Tang,et al.  Self-Powered Temperature Sensor with Seebeck Effect Transduction for Photothermal-Thermoelectric Coupled Immunoassay. , 2020, Analytical chemistry.

[19]  D. Tang,et al.  ZIF-8-Assisted NaYF4:Yb,Tm@ZnO Converter with Exonuclease III-Powered DNA Walker for Near-Infrared Light Responsive Biosensor. , 2019, Analytical chemistry.

[20]  S. Reich,et al.  Polystyrene nanofibers for nonwoven porous building insulation materials , 2019, Engineering Reports.

[21]  Ke Chen,et al.  Electrochemical Sensors Fabricated by Electrospinning Technology: An Overview , 2019, Sensors.

[22]  J. Fernández-Real,et al.  Circulating Soluble CD36 is Similar in Type 1 and Type 2 Diabetes Mellitus versus Non-Diabetic Subjects , 2019, Journal of clinical medicine.

[23]  Younan Xia,et al.  Electrospinning and Electrospun Nanofibers: Methods, Materials, and Applications. , 2019, Chemical reviews.

[24]  P. Shirage,et al.  Redox additive enhanced capacitance: Multi-walled carbon nanotubes/polyaniline nanocomposite based symmetric supercapacitors for rapid charge storage , 2019, Applied Surface Science.

[25]  Ji Man Kim,et al.  EDTA-functionalized KCC-1 and KIT-6 mesoporous silicas for Nd3+ ion recovery from aqueous solutions , 2018, Journal of Industrial and Engineering Chemistry.

[26]  L. Mattoso,et al.  Ultrasensitive biosensor based on polyvinylpyrrolidone/chitosan/reduced graphene oxide electrospun nanofibers for 17α – Ethinylestradiol electrochemical detection , 2018, Applied Surface Science.

[27]  Xue Zhu,et al.  Sensitive detection of cytokine in complex biological samples by using MB track mediated DNA walker and nicking enzyme assisted signal amplification method combined biosensor. , 2018, Talanta.

[28]  S. Timur,et al.  An electrospun nanofiber matrix based on organo-clay for biosensors: PVA/PAMAM-Montmorillonite , 2018, Applied Surface Science.

[29]  A. N. Chakoli,et al.  Collagen/aminated MWCNTs nanocomposites for biomedical applications , 2018, Materials Today Communications.

[30]  Yang Wu,et al.  Chitosan-rectorite nanospheres immobilized on polystyrene fibrous mats via alternate electrospinning/electrospraying techniques for copper ions adsorption , 2017 .

[31]  Kelley J. Rountree,et al.  A Practical Beginner’s Guide to Cyclic Voltammetry , 2017 .

[32]  Petr Slepička,et al.  Surface Modification of Polymer Substrates for Biomedical Applications , 2017, Materials.

[33]  Yoonkyung Park,et al.  Nanoscale Investigation of Generation 1 PAMAM Dendrimers Interaction with a Protein Nanopore , 2017, Scientific Reports.

[34]  G. Mckay,et al.  Synthesis and characterization of PAMAM/CNT nanocomposite as a super-capacity adsorbent for heavy metal (Ni2 +, Zn2 +, As3 +, Co2 +) removal from wastewater , 2016 .

[35]  Jean-Louis Marty,et al.  Label-Free Aptasensors for the Detection of Mycotoxins , 2016, Sensors.

[36]  Cross-Sectional Guidelines for Therapy with Blood Components and Plasma Derivatives: Chapter 5 Human Albumin - Revised , 2016, Transfusion Medicine and Hemotherapy.

[37]  D. Martuzevicius,et al.  The comparative study of aerosol filtration by electrospun polyamide, polyvinyl acetate, polyacrylonitrile and cellulose acetate nanofiber media , 2016 .

[38]  S. Timur,et al.  A sandwich-type assay based on quantum dot/aptamer bioconjugates for analysis of E. Coli O157:H7 in microtiter plate format , 2016 .

[39]  Dongxue Han,et al.  Intercorrelated Superhybrid of AgBr Supported on Graphitic‐C3N4‐Decorated Nitrogen‐Doped Graphene: High Engineering Photocatalytic Activities for Water Purification and CO2 Reduction , 2015, Advanced materials.

[40]  Thomas Emrich,et al.  Charge-mediated influence of the antibody variable domain on FcRn-dependent pharmacokinetics , 2014, Proceedings of the National Academy of Sciences.

[41]  M. K. Sezgintürk,et al.  A novel impedimetric disposable immunosensor for rapid detection of a potential cancer biomarker. , 2014, International journal of biological macromolecules.

[42]  S. Timur,et al.  Modified gold surfaces by 6-(ferrocenyl)hexanethiol/dendrimer/gold nanoparticles as a platform for the mediated biosensing applications. , 2013, Materials science & engineering. C, Materials for biological applications.

[43]  S. Yim,et al.  Simple quantification of surface carboxylic acids on chemically oxidized multi-walled carbon nanotubes , 2013 .

[44]  A. Venyaminova,et al.  Fluorecently labeled bionanotransporters of nucleic acid based on carbon nanotubes , 2012, 1208.3570.

[45]  J. Dekker,et al.  Plasma sCD36 is associated with markers of atherosclerosis, insulin resistance and fatty liver in a nondiabetic healthy population , 2012, Journal of internal medicine.

[46]  P. Akkaramongkolporn,et al.  Effects of processing parameters on morphology of electrospun polystyrene nanofibers , 2012, Korean Journal of Chemical Engineering.

[47]  Kadir Damar,et al.  Modified gold surfaces by poly(amidoamine) dendrimers and fructose dehydrogenase for mediated fructose sensing. , 2011, Talanta.

[48]  S. Timur,et al.  Gold nanoparticle modified conducting polymer of 4-(2,5-di(thiophen-2-yl)-1H-pyrrole-1-l) benzenamine for potential use as a biosensing material. , 2011, Food chemistry.

[49]  T. Saleh.  The influence of treatment temperature on the acidity of MWCNT oxidized by HNO3 or a mixture of HNO3/H2SO4 , 2011 .

[50]  P. Stenvinkel,et al.  Serum soluble CD36, assessed by a novel monoclonal antibody-based sandwich ELISA, predicts cardiovascular mortality in dialysis patients. , 2010, Clinica chimica acta; international journal of clinical chemistry.

[51]  A. Telefoncu,et al.  Maltose biosensing based on co-immobilization of alpha-glucosidase and pyranose oxidase. , 2010, Bioelectrochemistry.

[52]  Marc De Loose,et al.  Trends in quality in the analytical laboratory. II. Analytical method validation and quality assurance , 2004 .

[53]  M. Schöning,et al.  Label-Free Biosensing: Advanced Materials, Devices and Applications , 2018 .

[54]  J. Lima,et al.  Recent developments, characteristics and potential applications of screen-printed electrodes in pharmaceutical and biological analysis. , 2016, Talanta.