An assessment of in vitro androgenic activity and the identification of environmental androgens in United Kingdom estuaries

Environmental androgens are a group of compounds that to date have received very little attention. In this study, a yeast-based androgen screen (YAS) was used to determine the level of in vitro androgenic activity in seven United Kingdom estuaries. Surface water, sediment pore water, and sediment particulate material solvent extracts collected from Southampton Water, the Thames, Mersey, Tees, Tyne, Clyde, and Forth were tested for in vitro androgenic activity. Eleven of the 41 surface water samples collected displayed androgenic activity >2 ng dihydrotestosterone (DHT) equivalents/L (3-9 ng DHT/L), while eight of the 39 sediment pore waters collected showed activity >45 ng DHT/L (51-187 ng DHT/L). High levels of androgenic activity were determined in the solvent extracts of sediments, with 10 of 39 samples exhibiting a level of androgenic activity >454 ng DHT/kg (1,020-15,300 ng DHT/kg). In vitro YAS testing of five selected sewage treatment works (STW) effluents entering these estuaries showed that measurable levels (34-635 ng DHT/L) of androgenic activity were observed in those receiving only primary treatment (Howdon STW and Irvine Valley Sewer) at the time of the survey. A toxicity identification evaluation (TIE) study of Irvine Valley Sewer effluent using the YAS assay was used to identify the natural steroids/steroid metabolites dehydrotestosterone, androstenedione, androstanedione, 5beta-androstane-3alpha,11beta-diol-17-one, androsterone, and epi-androsterone as responsible for 99% of the in vitro activity determined in the effluent.

[1]  M. E. Gill,et al.  Abnormalities in the reproductive health of flounder Platichthys flesus exposed to effluent from a sewage treatment works , 1997 .

[2]  S. Bortone,et al.  Masculinization of Mosquitofish as an Indicator of Exposure to Kraft Mill Effluent , 1997, Bulletin of environmental contamination and toxicology.

[3]  D. A. Black,et al.  Abnormal Expression of Secondary Sex Characters in a Population of Mosquitofish, Gambusia affinis holbrooki: Evidence for Environmentally-induced Masculinization , 1980 .

[4]  D. G. Joakim Larsson,et al.  More male fish embryos near a pulp mill , 2000 .

[5]  C. Haux,et al.  An 11-ketotestosterone induced kidney-secreted protein: the nest building glue from male three-spined stickleback, Gasterosteus aculeatus , 2004, Fish Physiology and Biochemistry.

[6]  A. Scott,et al.  Survey of estrogenic activity in United Kingdom estuarine and coastal waters and its effects on gonadal development of the flounder Platichthys flesus , 1999 .

[7]  J. Sumpter,et al.  Several environmental oestrogens are also anti-androgens. , 1998, The Journal of endocrinology.

[8]  J. Sumpter,et al.  Estrogenic Effects of Effluents from Sewage Treatment Works , 1994 .

[9]  J. A. Kemppainen,et al.  Identification of androstenedione in a river containing paper mill effluent , 2001, Environmental toxicology and chemistry.

[10]  M. Waldock,et al.  Characterization of estrogenic compounds in water samples collected from United Kingdom estuaries , 2001, Environmental toxicology and chemistry.

[11]  E. Antonopoulou,et al.  Effectiveness of several androgens in stimulating kidney hypertrophy, a secondary sexual character, in castrated male three-spined sticklebacks, Gasterosteus aculeatus , 1993 .

[12]  Kevin V. Thomas,et al.  Identification of toxic substances in United Kingdom estuaries , 1999 .

[13]  S. Bortone,et al.  Morphological and behavioral characters in mosquitofish as potential bioindication of exposure to kraft mill effluent , 1989, Bulletin of environmental contamination and toxicology.