Utilization of Natural Fucosylated Oligosaccharides by Three Novel α-l-Fucosidases from a Probiotic Lactobacillus casei Strain

ABSTRACT Three putative α-l-fucosidases encoded in the Lactobacillus casei BL23 genome were cloned and purified. The proteins displayed different abilities to hydrolyze natural fucosyloligosaccharides like 2′-fucosyllactose, H antigen disaccharide, H antigen type II trisaccharide, and 3′-, 4′-, and 6′-fucosyl-GlcNAc. This indicated a possible role in the utilization of oligosaccharides present in human milk and intestinal mucosa.

[1]  C. Lebrilla,et al.  In Vitro Fermentation of Breast Milk Oligosaccharides by Bifidobacterium infantis and Lactobacillus gasseri , 2006, Applied and Environmental Microbiology.

[2]  M. Hattori,et al.  Complete Genome Sequence of the Probiotic Lactobacillus rhamnosus ATCC 53103 , 2009, Journal of Bacteriology.

[3]  Mi-Hwa Oh,et al.  Complete Genome Sequence of Bifidobacterium longum subsp. longum KACC 91563 , 2011, Journal of bacteriology.

[4]  H. Kumagai,et al.  Two distinct alpha-L-fucosidases from Bifidobacterium bifidum are essential for the utilization of fucosylated milk oligosaccharides and glycoconjugates. , 2009, Glycobiology.

[5]  A. Margolles,et al.  Mucin Degradation by Bifidobacterium Strains Isolated from the Human Intestinal Microbiota , 2008, Applied and Environmental Microbiology.

[6]  J. Gibrat,et al.  Complete Genome Sequence of the Probiotic Lactobacillus casei Strain BL23 , 2010, Journal of bacteriology.

[7]  J. Chapman,et al.  The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome , 2008, Proceedings of the National Academy of Sciences.

[8]  C. Lebrilla,et al.  Glycoprofiling of bifidobacterial consumption of human milk oligosaccharides demonstrates strain specific, preferential consumption of small chain glycans secreted in early human lactation. , 2007, Journal of agricultural and food chemistry.

[9]  H. Kumagai,et al.  Molecular Cloning and Characterization of Bifidobacterium bifidum 1,2-α-l-Fucosidase (AfcA), a Novel Inverting Glycosidase (Glycoside Hydrolase Family 95) , 2004, Journal of bacteriology.

[10]  J. Lowe,et al.  Fucose: biosynthesis and biological function in mammals. , 2003, Glycobiology.