Elastic Light Single-Scattering Spectroscopy for the Detection of Cervical Precancerous Ex vivo

Potential application of elastic light single-scattering spectroscopy (ELSSS) for differentiating high-grade squamous intraepithelial lesions (HSIL) from non-HSIL tissues was investigated. An ELSSS system was used to acquire spectra from cervix tissues. A single-fiber optical probe with a diameter of 100 μm was used for both delivery and detection of white light to and from the cervix tissue. Spectroscopic measurements were acquired from 95 ex vivo biopsy samples of 60 pap smear positive patients and normal cervix tissue from 10 patients after hysterectomy were used as a negative control group. Spectroscopic results of 95 cervix biopsy were compared to the histopathology of the biopsy samples. Sensitivity and specificity of the ELSSS system in the differentiation of HSIL and non-HSIL tissues are 87.5% and 45.6%, respectively, for the pap smear and colposcopy positive biopsy samples. The ELSSS system has the potential for use in real-time diagnosis of HSIL tissues as an adjunct to Papanicolaou test (pap smear) and colposcopy.

[1]  Michele Follen,et al.  Combined reflectance and fluorescence spectroscopy for in vivo detection of cervical pre-cancer. , 2005, Journal of biomedical optics.

[2]  L Burke,et al.  Identification of cervical intraepithelial neoplasia (CIN) using UV‐excited fluorescence and diffuse‐reflectance tissue spectroscopy , 2001, Lasers in surgery and medicine.

[3]  J. Mourant,et al.  Particle size analysis of turbid media with a single optical fiber in contact with the medium to deliver and detect white light. , 2001, Applied optics.

[4]  R. Richards-Kortum,et al.  Light scattering from cervical cells throughout neoplastic progression: influence of nuclear morphology, DNA content, and chromatin texture. , 2003, Journal of biomedical optics.

[5]  J Beuthan,et al.  The spatial variation of the refractive index in biological cells. , 1996, Physics in medicine and biology.

[6]  Murat Canpolat,et al.  Detecting skin malignancy using elastic light scattering spectroscopy , 2007, European Conference on Biomedical Optics.

[7]  Michele Follen,et al.  Model-based analysis of clinical fluorescence spectroscopy for in vivo detection of cervical intraepithelial dysplasia. , 2006, Journal of biomedical optics.

[8]  Irene Georgakoudi,et al.  Trimodal spectroscopy for the detection and characterization of cervical precancers in vivo. , 2002, American journal of obstetrics and gynecology.

[9]  Angela A. Eick,et al.  Mechanisms of light scattering from biological cells relevant to noninvasive optical-tissue diagnostics. , 1998, Applied optics.

[10]  Michael B. Wallace,et al.  Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution , 1998 .

[11]  M. Canpolat,et al.  Diagnosis abnormal cervical tissue by elastic light scattering spectroscopy and correlation with the results of Pap-smear examination , 2010, 2010 15th National Biomedical Engineering Meeting.

[12]  L Irwig,et al.  Meta-analysis of Pap test accuracy. , 1995, American journal of epidemiology.

[13]  Michele Follen,et al.  Reflectance spectroscopy for in vivo detection of cervical precancer. , 2002, Journal of biomedical optics.

[14]  Judith R Mourant,et al.  In vivo light scattering for the detection of cancerous and precancerous lesions of the cervix. , 2009, Applied optics.

[15]  M. Canpolat,et al.  Intra-operative brain tumor detection using elastic light single-scattering spectroscopy: a feasibility study. , 2009, Journal of biomedical optics.

[16]  Michael S. Feld,et al.  Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ , 1999 .

[17]  M. Canpolat,et al.  Differentiation of Melanoma from Non-Cancerous Tissue in an Animal Model Using Elastic Light Single-Scattering Spectroscopy , 2008, Technology in cancer research & treatment.

[18]  Arjen Amelink,et al.  Single-scattering spectroscopy for the endoscopic analysis of particle size in superficial layers of turbid media. , 2003, Applied optics.