Raman Spectroscopic Detection in Continuous Micro-Flow Using a Chip-Integrated Silver Electrode as Electrically Regenerable SERS Substrate.

An electrochemical approach to enable surface-enhanced Raman spectroscopy (SERS) detection in continuous microflow is presented. This is achieved by the integration of a silver electrode as SERS-substrate in a microfluidic chip device. By the application of actuation pulses of about 4 V otherwise irreversibly adsorbed analytes are stripped off, which enables quasi-realtime SERS-detection in a continuous microflow. The approach opens up a way for in situ SERS monitoring of com-pounds in microflow with high application potential in micro separation techniques like HPLC and lab-on-a-chip devices.

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