A transmissive laser speckle imaging technique for measuring deep tissue blood flow: An example application in finger joints

Laser speckle perfusion imaging (LSPI) is a minimally invasive optical measure of relative changes in blood flow, providing real‐time, high resolution, two‐dimensional maps of vascular structure. Standard LSI imaging uses a light‐reflective geometry that limits the measurement to a thin surface layer of 0.2–1 mm. The objective of this study was to test a new LSI instrument geometry with the laser source opposed to the image capture plane (light transmissive). Captured light then travels the entire tissue thickness (10–15 mm), sampling much deeper regions of interest than conventional optical imaging techniques.

[1]  J. Tulip,et al.  A comparison of two laser-based methods for determination of burn scar perfusion: laser Doppler versus laser speckle imaging. , 2005, Burns : journal of the International Society for Burn Injuries.

[2]  R D Sturrock,et al.  Metacarpophalangeal joints in rheumatoid arthritis: laser Doppler imaging--initial experience. , 2001, Radiology.

[3]  T. Pullar,et al.  Clinical Improvement in Rheumatoid Arthritis Is Associated with Healthier Microvascular Function in Patients Who Respond to Antirheumatic Therapy , 2010, The Journal of Rheumatology.

[4]  P O Byrne,et al.  A laser Doppler scanner for imaging blood flow in skin. , 1991, Journal of biomedical engineering.

[5]  J. Tulip,et al.  Endoscopic laser speckle imaging of tissue blood flow: Applications in the human knee , 2006, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[6]  J D Briers,et al.  Capillary Blood Flow Monitoring Using Laser Speckle Contrast Analysis (LASCA). , 1999, Journal of biomedical optics.

[7]  J. Briers,et al.  Flow visualization by means of single-exposure speckle photography , 1981 .

[8]  John E. W. Mayhew,et al.  Refinement of optical imaging spectroscopy algorithms using concurrent BOLD and CBV fMRI , 2009, NeuroImage.

[9]  M. Moskowitz,et al.  Dynamic Imaging of Cerebral Blood Flow Using Laser Speckle , 2001, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[10]  G. Kobelt,et al.  The burden of rheumatoid arthritis and access to treatment: health burden and costs , 2007, The European Journal of Health Economics.

[11]  G. Nilsson,et al.  Laser Doppler perfusion imaging by dynamic light scattering , 1993, IEEE Transactions on Biomedical Engineering.

[12]  J. Tulip,et al.  Comparison of laser speckle and laser Doppler perfusion imaging: Measurement in human skin and rabbit articular tissue , 2002, Medical and Biological Engineering and Computing.

[13]  J. Braun,et al.  Lack of a difference in increased capillary blood cell velocity in the skin over proximal interphalangeal joints between rheumatoid arthritis and osteoarthritis. , 2005, Microvascular research.

[14]  Wiley Interscience,et al.  Endoscopic laser imaging of tissue perfusion: New instrumentation and technique , 2003, Lasers in surgery and medicine.

[15]  Kevin R. Forrester,et al.  A laser speckle imaging technique for measuring tissue perfusion , 2004, IEEE Transactions on Biomedical Engineering.

[16]  M. Moskowitz,et al.  Laser Speckle Flowmetry for the Study of Cerebrovascular Physiology in Normal and Ischemic Mouse Cortex , 2004, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[17]  W. Ferrell,et al.  Evaluation of laser Doppler imaging to measure blood flow in knee ligaments of adult rabbits , 1996, Medical and Biological Engineering and Computing.

[18]  Qingming Luo,et al.  Spatiotemporal laser speckle contrast analysis for blood flow imaging with maximized speckle contrast. , 2010, Journal of biomedical optics.

[19]  Gert E. Nilsson,et al.  A New Instrument for Continuous Measurement of Tissue Blood Flow by Light Beating Spectroscopy , 1980, IEEE Transactions on Biomedical Engineering.

[20]  B. Kruijt,et al.  Laser speckle imaging of dynamic changes in flow during photodynamic therapy , 2006, Lasers in Medical Science.

[21]  M. Doschak,et al.  In vivo comparison of scanning technique and wavelength in laser Doppler perfusion imaging: Measurement in knee ligaments of adult rabbits , 1997, Medical and Biological Engineering and Computing.

[22]  Toshimitsu Asakura,et al.  Velocity measurement of a diffuse object by using time-varying speckles , 1976 .

[23]  Gert E. Nilsson,et al.  Evaluation of a Laser Doppler Flowmeter for Measurement of Tissue Blood Flow , 1980, IEEE Transactions on Biomedical Engineering.

[24]  B. Ruth,et al.  Velocity measurement by the laser speckle method using optical fibres , 1987 .

[25]  R. Bray,et al.  ACL deficiency impairs the vasoconstrictive efficacy of neuropeptide Y and phenylephrine in articular tissues: a laser speckle perfusion imaging study. , 2005, Journal of applied physiology.