ANTIPROLIFERATIVE, ANTIOXIDANT ACTIVITY AND TOTAL PHENOLIC CONTENT OF MITRAGYNA PARVIFOLIA (ROXB.) KORTH

Objective: In the present study bark and leaves of Mitragynaparvifolia (Roxb.) Korthwere evaluated for total phenolic content, total flavonoid content,antioxidant potential, lipid peroxidation and antiproliferative effect on HeLa cell lines. Methods: Dried bark and leaves were suspended in five different solvent systemsviz; distilled water, methanol, acetone, ethyl acetate and hexane . Total phenolic content was estimated usingFolin–Ciocalteau reagent and phenolic profiling byusing high performance liquid chromatography (HPLC). Antioxidant potential and flavonoids estimation were investigated usingDPPHradical scavenging activity and aluminium chloride method respectively. Further lipid peroxidation and antiproliferative effect were observed using TBARS and MTT assay, followed by cell morphology using Giemsa and Acridine orange staining. Results:The acetone extract of bark(57.08 ± 6.16 mg/mL) and leaf (60.1 ± 3.74 mg/mL)exhibited the highest phenolic content. Distilled water extractsof bark (94 ± 0.05%) and leaf (95.63 ± 0.34%) showed maximum antioxidant potential. Percent protection was observed maximum in acetone extracts of bark(19.04±0.02%) and leaf (25.11±0.09%). Total flavonoid content was found maximum in distilled water leaf extracts(0.869±0.001 mg/mL). Acetone extracts showed high cytotoxic effect on HeLa cells compared to other extracts with very minimal or no cytotoxicity contributed by the vehicle (acetone) itself in comparison to positive control of 5% ricin. Conclusion:Acetone extracts exhibited highest cytotoxic effect on HeLa cells compared to other extracts. These results also indicated that acetone extract have highest phenolic content and percent protection in lipid peroxidation. Thus Mitragynaparvifolia’s acetone extracts can attribute to one of the several bioactive compounds which can be further studied in detail.

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