Synthesis, characterization, bioactivity and antibacterial studies of silver doped calcium borosilicate glass-ceramics

Bioactive glass-ceramics 45.8 mol% SiO– 45.8 CaO – 8.4 B2O3 doped with Ag2O were synthesized by sol-gel method. The glass-ceramic nature of samples was confirmed by X-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis. Fourier transform infrared (FT-IR) spectra reveal the probable stretching and bending vibration modes of silicate and borate groups. UV-Visible spectra reveal the presence of Ag+ ions and metallic Ag in the glass matrix for Ag2O doped ceramic sample. Biocompatibility of the glass nature of samples was studied by soaking of samples in Dulbecco’s Modified Eagle’s Medium (DMEM) with subsequent XRD studies. It was found that bone-like apatite formation on the glasses after soaked in DMEM. Antibacterial studies of glass ceramics powder against gram positive and negative microorganisms were carried out.Bioactive glass-ceramics 45.8 mol% SiO– 45.8 CaO – 8.4 B2O3 doped with Ag2O were synthesized by sol-gel method. The glass-ceramic nature of samples was confirmed by X-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis. Fourier transform infrared (FT-IR) spectra reveal the probable stretching and bending vibration modes of silicate and borate groups. UV-Visible spectra reveal the presence of Ag+ ions and metallic Ag in the glass matrix for Ag2O doped ceramic sample. Biocompatibility of the glass nature of samples was studied by soaking of samples in Dulbecco’s Modified Eagle’s Medium (DMEM) with subsequent XRD studies. It was found that bone-like apatite formation on the glasses after soaked in DMEM. Antibacterial studies of glass ceramics powder against gram positive and negative microorganisms were carried out.

[1]  A. Gedanken,et al.  Understanding the antibacterial mechanism of CuO nanoparticles: revealing the route of induced oxidative stress. , 2012, Small.

[2]  L. C. Li,et al.  Strontium borate glass: potential biomaterial for bone regeneration , 2010, Journal of The Royal Society Interface.

[3]  Y. Park,et al.  Antibacterial Activity and Mechanism of Action of the Silver Ion in Staphylococcus aureus and Escherichia coli , 2008, Applied and Environmental Microbiology.

[4]  D. Zhao,et al.  The in-vitro bioactivity of mesoporous bioactive glasses. , 2006, Biomaterials.

[5]  Carla Renata Arciola,et al.  The significance of infection related to orthopedic devices and issues of antibiotic resistance. , 2006, Biomaterials.

[6]  K. C. Bhainsa,et al.  Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus. , 2006, Colloids and surfaces. B, Biointerfaces.

[7]  Seong-Geun Oh,et al.  Preparation and antibacterial effects of Ag-SiO2 thin films by sol-gel method. , 2003, Biomaterials.

[8]  F. Cui,et al.  A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. , 2000, Journal of biomedical materials research.

[9]  H. Kozuka,et al.  Antibacterial silver-containing silica glass prepared by sol-gel method. , 2000, Biomaterials.

[10]  L L Hench,et al.  Bioactive materials: the potential for tissue regeneration. , 1998, Journal of biomedical materials research.

[11]  Larry L. Hench,et al.  Bonding mechanisms at the interface of ceramic prosthetic materials , 1971 .

[12]  L. Hench,et al.  Characterization of melt-derived 45S5 and sol-gel-derived 58S bioactive glasses. , 2001, Journal of biomedical materials research.

[13]  D. Greenspan,et al.  Processing and properties of sol-gel bioactive glasses. , 2000, Journal of biomedical materials research.