Protective efficacy of active compounds from Phyllanthus amarus against white spot syndrome virus in freshwater crab (Paratelphusa hydrodomous)

Repeatedly the shrimp culturing industry faces serious economic loss due to white spot disease caused by white spot syndrome virus (WSSV) in penaeid shrimp. The present study was carried out to determine the antiviral activity of traditional Indian medicinal herb Phyllanthus amarus against WSSV in freshwater crab, Paratelphusa hydrodomous (Herbst). For experimental challenge, crabs were injected with three different solvent extracts of P. amarus (aqueous, acetone and petroleum ether) along with WSSV. The efficacy of the extracts was confirmed by bioassay, histopathology and reverse transcriptase – polymerase chain reaction (RT-PCR). The overall results of the present study confirmed that acetone and petroleum ether extracts of P. amarus have significant antiviral effect against WSSV.

[1]  B. Belton,et al.  Comparative study of the reproductive performance and White Spot Syndrome Virus (WSSV) status of black tiger shrimp (Penaeus monodon) collected from the Bay of Bengal , 2014 .

[2]  Vikash Kumar,et al.  Immunostimulants for Aquaculture Health Management , 2013 .

[3]  S. Vimal,et al.  In vitro white spot syndrome virus (WSSV) replication in explants of the heart of freshwater crab, Paratelphusa hydrodomous. , 2012, Journal of virological methods.

[4]  T. Marudhupandi,et al.  The effect of fucoidan from brown seaweed Sargassum wightii on WSSV resistance and immune activity in shrimp Penaeus monodon (Fab). , 2012, Fish & shellfish immunology.

[5]  V. Dixit,et al.  Phyllanthus amarus: ethnomedicinal uses, phytochemistry and pharmacology: a review. , 2011, Journal of ethnopharmacology.

[6]  R. Philip,et al.  In vivo screening of mangrove plants for anti WSSV activity in Penaeus monodon, and evaluation of Ceriops tagal as a potential source of antiviral molecules , 2011 .

[7]  A. S. S. Hameed,et al.  Studies on the immunomodulatory effect of extract of Cyanodon dactylon in shrimp, Penaeus monodon, and its efficacy to protect the shrimp from white spot syndrome virus (WSSV). , 2008, Fish & shellfish immunology.

[8]  S. R. Kumar,et al.  Screening the antiviral activity of Indian medicinal plants against white spot syndrome virus in shrimp , 2007 .

[9]  G. Immanuel,et al.  Influence of selected Indian immunostimulant herbs against white spot syndrome virus (WSSV) infection in black tiger shrimp, Penaeus monodon with reference to haematological, biochemical and immunological changes. , 2006, Fish & shellfish immunology.

[10]  C. T. Achuthankutty,et al.  Treatment of white spot syndrome virus (WSSV) in penaeid shrimp aquaculture using plant extract , 2004 .

[11]  R. B. Narayanan,et al.  A rapid non-enzymatic method of DNA extraction for PCR detection of white spot syndrome virus in shrimp , 2003 .

[12]  R. Kuttan,et al.  Hypoglycemic effect of methanol extract of Phyllanthus amarus Schum & Thonn on alloxan induced diabetes mellitus in rats and its relation with antioxidant potential. , 2002, Indian journal of experimental biology.

[13]  A. S. S. Hameed,et al.  White spot syndrome virus (WSSV) in two species of freshwater crabs (Paratelphusa hydrodomous and P. pulvinata) , 2001 .

[14]  A. S. S. Hameed,et al.  Antibiotic resistance in bacteria isolated from Artemia nauplii and efficacy of formaldehyde to control bacterial load , 2000 .

[15]  M. Yoshimizu,et al.  Antiviral Activity of Several Thai Traditional Herb Extracts against Fish Pathogenic Viruses , 1996 .

[16]  C. Lo,et al.  Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan , 1995 .

[17]  B. Blumberg,et al.  EFFECT OF PHYLLANTHUS AMARUS ON CHRONIC CARRIERS OF HEPATITIS B VIRUS , 1988, The Lancet.

[18]  P. A. Ganeshpure,et al.  Structure and synthesis of hypophyllanthin, nirtetralin, phyltetralin and lintetralin , 1981 .

[19]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.