Construction of basic logic gates based on rapid detection of transferrin conformations

Abstract A simple technique for constructing different protein logic gates (OR and INHIBIT) using Fe3+ and/or Al3+ to regulate changes in the conformation of transferrin. Logic gates are easy to control and also display real-time responses to inputs. This study provides new insights into the use of different conformations to design transferrin logic gates and provides a means to further detect transferrin in serum systems.

[1]  Wei Guo,et al.  Transferrin serves as a mediator to deliver organometallic ruthenium(II) anticancer complexes into cells. , 2013, Inorganic chemistry.

[2]  Shaojun Dong,et al.  An intelligent universal system yields double results with half the effort for engineering a DNA “Contrary Logic Pairs” library and various DNA combinatorial logic circuits , 2017 .

[3]  Juyoung Yoon,et al.  Fluorescent molecular logic gates using microfluidic devices. , 2008, Angewandte Chemie.

[4]  Jing Zhang,et al.  Interaction of chromium(III) or chromium(VI) with catalase and its effect on the structure and function of catalase: An in vitro study. , 2018, Food chemistry.

[5]  Qianfan Yang,et al.  Recent Progress in Fluorescence Signal Design for DNA-Based Logic Circuits. , 2019, Chemistry.

[6]  Yalin Tang,et al.  Verification of specific G-quadruplex structure by using a novel cyanine dye supramolecular assembly: I. recognizing mixed G-quadruplex in human telomeres. , 2009, Chemical communications.

[7]  Catalina Matias,et al.  Citrate and albumin facilitate transferrin iron loading in the presence of phosphate. , 2017, Journal of inorganic biochemistry.

[8]  Yalin Tang,et al.  Chiral transformation of achiral J-aggregates of a cyanine dye templated by human serum albumin. , 2007, Chemphyschem : a European journal of chemical physics and physical chemistry.

[9]  Yan Liu,et al.  Visual detection of potassium by a cyanine dye supramolecular aggregate responsive to G-quadruplex motif transition. , 2012, The Analyst.

[10]  Wei Tao Huang,et al.  Boolean Logic Tree of Label-Free Dual-Signal Electrochemical Aptasensor System for Biosensing, Three-State Logic Computation, and Keypad Lock Security Operation. , 2017, Analytical chemistry.

[11]  H. Tamary,et al.  Labile plasma iron as an indicator of patient adherence to iron chelation treatment. , 2018, Blood cells, molecules & diseases.

[12]  Yalin Tang,et al.  Visual detection of mercury(II) based on recognition of the G-quadruplex conformational transition by a cyanine dye supramolecule. , 2015, The Analyst.

[13]  Raphael D. Levine,et al.  A full-adder based on reconfigurable DNA-hairpin inputs and DNAzyme computing modules , 2014 .

[14]  Kamil Strzelak,et al.  Photometric flow analysis system for biomedical investigations of iron/transferrin speciation in human serum. , 2017, Analytica chimica acta.

[15]  E. Tajkhorshid,et al.  Structural basis for iron piracy by pathogenic Neisseria , 2012, Nature.

[16]  Joseph Bertolini,et al.  Transferrin: structure, function and potential therapeutic actions. , 2005, Drug discovery today.

[17]  Richard G Compton,et al.  Optimising amperometric pH sensing in blood samples: an iridium oxide electrode for blood pH sensing. , 2019, The Analyst.

[18]  Jie Zheng,et al.  Mimicking the binding and unbinding of Fe3+ with transferrin using a single biomimetic nanochannel. , 2013, Chemical communications.

[19]  Yalin Tang,et al.  Specific identification of human transferrin conformations using a cyanine dye supramolecular assembly , 2017 .

[20]  S. Everse,et al.  How the binding of human transferrin primes the transferrin receptor potentiating iron release at endosomal pH , 2011, Proceedings of the National Academy of Sciences.

[21]  Quan Hao,et al.  Iron and bismuth bound human serum transferrin reveals a partially-opened conformation in the N-lobe , 2012, Scientific Reports.

[22]  Yuanyuan Du,et al.  A versatile DNA-supramolecule logic platform for multifunctional information processing , 2018, NPG Asia Materials.

[23]  R. MacGillivray,et al.  Intrinsic fluorescence reports a global conformational change in the N-lobe of human serum transferrin following iron release. , 2007, Biochemistry.

[24]  Carmen Rojas-Martínez,et al.  Babesia bigemina: Advances in continuous in vitro culture using serum-free medium supplemented with insulin, transferrin, selenite, and putrescine. , 2017, Parasitology international.