Lipase production from Aspergillus niger by solid-state fermentation using gingelly oil cake

Cultural conditions for the production of lipase by Aspergillus niger strain MTCC 2594 by solid-state fermentation using gingelly oil cake were standardized. A lipase activity of 363·6 U/g of dry substrate was obtained at 72 h under optimum conditions. Addition of various nitrogen sources, carbohydrates and inducers to the substrate was found to be ineffective. The enzyme was optimally active at pH 7·0 and 37°C, and was found to be stable between pH 4·0–10·0 and 4–50°C. The enzyme also showed remarkable stability in the presence of detergents and it could be effectively used for the removal of triglyceride soils in laundry.

[1]  D. Madamwar,et al.  Solid state fermentation for cellulases and β-glucosidase production by Aspergillus niger , 1989 .

[2]  S. V. Ramakrishna,et al.  Engineering aspects of solid state fermentation , 1985 .

[3]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[4]  L. A. Elson,et al.  A colorimetric method for the determination of glucosamine and chondrosamine. , 1933, The Biochemical journal.

[5]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[6]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[7]  P. V. Rao,et al.  Production of lipase by Candida rugosa in solid state fermentation. 1: determination of significant process variables , 1993 .

[8]  E. B. Bagley,et al.  Production of aflatoxin in corn by a large‐scale solid‐substrate fermentation process , 1979 .

[9]  C. Hesseltine,et al.  Biotechnology report: Solid state fermentations , 1972, Biotechnology and bioengineering.

[10]  B. Lee,et al.  Production and characterization of esterase-lipase of Lactobacillus casei subsp. pseudoplantarum LE2 , 1989 .

[11]  P. V. Rao,et al.  Production of lipase by Candida rugosa in solid state fermentation. 2: Medium optimization and effect of aeration , 1993 .

[12]  B. K. Lonsane,et al.  Efficient and simple extraction of mouldy bran in a pulsed column extractor for recovery of amyloglucosidase in concentrated form , 1991 .

[13]  T. Fujii,et al.  Studies on applications of lipolytic enzyme in detergency I. Effect of lipase fromCandida cylindracea on removal of olive oil from cotton fabric , 1986 .