Partition coefficients of 2,2,6,6‐tetramethylpiperidine‐1‐oxyl (TEMPO) in erythrocyte lipids in multiple sclerosis

Electron spin resonance (ESR) was used in a search for a possible generalized membrane defect underlying myelin instability in multiple sclerosis (MS). In contrast to most ESR studies of biological membranes, a small, nonelectrolyte spin label, TEMPO, was chosen; its partition coefficient was determined in extracted erythrocyte lipids from MS patients and normal controls. No differences were observed. The advantages and limitations of TEMPO in comparison with lipid spin labels as probes for the study of biological membranes are discussed.

[1]  B. Berner,et al.  The electron spin resonance line width method for measuring diffusion. A critique , 1979 .

[2]  C. Polnaszek,et al.  Spin labels in membranes. Problems in practice. , 1978, Biochimica et biophysica acta.

[3]  C. Polnaszek,et al.  Analysis of the factors determining the EPR spectra of spin probes that partition between aqueous and lipid phases , 1978 .

[4]  F. H. Tyler,et al.  Electron spin resonance studies of erythrocytes from patients with Duchenne muscular dystrophy. , 1978, The Journal of clinical investigation.

[5]  L. Dalton,et al.  Erythrocyte membrane abnormalities in Duchenne muscular dystrophy monitored by saturation transfer electron paramagnetic resonance spectroscopy. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[6]  J. Seelig.  Deuterium magnetic resonance: theory and application to lipid membranes , 1977, Quarterly Reviews of Biophysics.

[7]  D. Stasiw,et al.  Quantitative osmotic fragility and disease states: a preliminary study. , 1977, The Journal of laboratory and clinical medicine.

[8]  B. J. Gaffney,et al.  Spin-Label Studies of Membranes , 1977 .

[9]  W. Cammer,et al.  Chemical Pathology of Diseases Involving Myelin , 1977 .

[10]  J. Mertin,et al.  Relevance of fatty acids in multiple sclerosis. , 1977, British medical bulletin.

[11]  A. Roses,et al.  Spin label study of erythrocyte membrane fluidity in myotonic and Duchenne muscular dystrophy and congenital myotonia , 1976, Nature.

[12]  D. B. Chesnut,et al.  On the use of the spin labeling technique in the study of erythrocyte membranes. , 1976, Biochimica et biophysica acta.

[13]  E. Gelmann,et al.  Physical properties of membrane lipids: biological relevance and regulation. , 1975, Bacteriological reviews.

[14]  A. Ross,et al.  Permeation of a spin-label phosphate into the human erythrocyte. , 1975, Biochemistry.

[15]  P. Lin,et al.  Focal erythrocyte membrane perturbations caused by nitroxide lipid analogues. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[16]  D. B. Chesnut,et al.  Electron spin resonance studies of erythrocytes from patients with myotonic muscular dystrophy. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[17]  J. Dix,et al.  Translational diffusion coefficient and partition coefficient of a spin-labeled solute in lecithin bilayer membranes. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[18]  H. Friedman,et al.  Hydrophobic nitroxide radicals as probes to investigate the hydrophobic interaction , 1974 .

[19]  B. Wisnieski,et al.  Physical properties of the lipid phase of membranes from cultured animal cells. , 1974, Journal of supramolecular structure.

[20]  S. Metcalfe,et al.  The interaction of small molecules with spin-labelled erythrocyte membranes. , 1970, Biochimica et biophysica acta.

[21]  M. Baur,et al.  ESR studies of DTBN in a model lipid water system , 1970 .

[22]  B. D. Ladbrooke,et al.  Thermal analysis of lipids, proteins and biological membranes. A review and summary of some recent studies. , 1969, Chemistry and physics of lipids.

[23]  P. R. Bevington,et al.  Data Reduction and Error Analysis for the Physical Sciences , 1969 .

[24]  J. Prineas.  RED BLOOD CELL SIZE IN MULTIPLE SCLEROSIS , 1968, Acta neurologica Scandinavica.

[25]  E. J. Field,et al.  Platelet stickiness in multiple sclerosis. , 1965, Lancet.

[26]  A. Dayan,et al.  ORGANIC OR FUNCTIONAL? , 1964, Lancet.

[27]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.