Expression of epidermal growth factor receptor in oral tumor

We have generated a monoclonal antibody to the epidermal growth factor receptor, designated 12-93. This antibody reacted with non-carbohydrate moiety of EGF-receptor (EGF-r) and inhibits the binding of EGF to its receptor. Using 12-93 IgG, expression of EGF-r in normal oral mucosa, normal salivary gland and oral cancer tumor tissues were determined by immunohistological studies.12-93 IgG staining appears largely restricted to basal cells on normal mucosa and ductal cells on normal salivary gland. On the other hand, cell membranes of all squamous cell carcinomas were uniformly stained but the intensity of staining was gradually decreased towards the center of cancer pearl.In salivary gland tumors, tumor cells especially exhibiting ductal formation were weakly but definitively stained with 12-93 IgG. These results strongly suggest the possibility that the expression of EGF-r may be involved in the malignant transformation of cells in oral salivary gland as well as oral mucosa. Furthermore, this antibody may be useful to study the expression of EGF-r in oral tumors.

[1]  N. Shimizu,et al.  High incidence of egf receptor hyperproduction in esophageal squamous‐cell carcinomas , 1987, International journal of cancer.

[2]  R. Derynck,et al.  Loss of growth responsiveness to epidermal growth factor and enhanced production of alpha‐transforming growth factors in ras‐transformed mouse mammary epithelial cells , 1987, Journal of cellular physiology.

[3]  S. Cohen,et al.  Epidermal growth factor , 1972, The Journal of investigative dermatology.

[4]  B. Gusterson,et al.  Over‐expression of the EGF receptor is a hallmark of squamous cell carcinomas , 1986, The Journal of pathology.

[5]  W. Yasui,et al.  Human epidermal growth factor in gastric carcinoma as a biologic marker of high malignancy. , 1986, Japanese journal of cancer research : Gann.

[6]  M. Waterfield,et al.  Expression of epidermal growth factor receptors on human cervical, ovarian, and vulval carcinomas. , 1986, Cancer research.

[7]  T. Bando,et al.  Expression of epidermal growth factor and transforming growth factor-β in a human salivary gland adenocarcinoma cell line , 1985 .

[8]  M. Bennett,et al.  EPIDERMAL-GROWTH-FACTOR RECEPTORS IN HUMAN BLADDER CANCER: COMPARISON OF INVASIVE AND SUPERFICIAL TUMOURS , 1985, The Lancet.

[9]  Anita B. Roberts,et al.  Autocrine growth factors and cancer , 1985, Nature.

[10]  M. Das,et al.  42,000-molecular weight EGF receptor has protein kinase activity , 1984, Nature.

[11]  B. Ozanne,et al.  Human squamous cell lung cancers express increased epidermal growth factor receptors. , 1984, The Journal of clinical investigation.

[12]  J. Wittliff,et al.  Epidermal growth factor binding by breast tumor biopsies and relationship to estrogen receptor and progestin receptor levels. , 1984, Cancer research.

[13]  P. Seeburg,et al.  Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells , 1984, Nature.

[14]  C. Heldin,et al.  Growth factors: Mechanism of action and relation to oncogenes , 1984, Cell.

[15]  A. Ullrich,et al.  Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences , 1984, Nature.

[16]  M. Sporn,et al.  Autocrine secretion and malignant transformation of cells. , 1980, The New England journal of medicine.

[17]  S. Cohen,et al.  Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes. , 1980, The Journal of biological chemistry.

[18]  E. Gresik.  Postnatal developmental changes in submandibular glands of rats and mice. , 1980, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[19]  J. Elder,et al.  Cellular localisation of human urogastrone/epidermal growth factor , 1978, Nature.

[20]  T. Barka,et al.  Immunocytochemical localization of epidermal growth factor in mouse submandibular gland. , 1977, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[21]  H. Herschman,et al.  Specific radiolabeling of a cell surface receptor for epidermal growth factor. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[22]  G. Todaro,et al.  Nerve growth factor receptors on human melanoma cells in culture. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[23]  G. Todaro,et al.  Transformation by murine and feline sarcoma viruses specifically blocks binding of epidermal growth factor to cells , 1976, Nature.