Role of carbon nano-materials in the analysis of biological materials by laser desorption/ionization-mass spectrometry.

At present, carbon nano-materials are being utilized in various procedures, especially in laser desorption/ionization-mass spectrometry (LDI-MS) for analyzing a range of analytes, which include peptides, proteins, metabolites, and polymers. Matrix-oriented LDI-MS techniques are very well established, with weak organic acids as energy-absorbing substances. Carbon materials, such as nano-tubes and fullerenes are being successfully applied in the small-mass range, where routine matrices have strong background signals. In addition, the role of carbon nano-materials is very well established in the fractionation and purification fields. Modified diamond powder and surfaces are utilized in binding peptides and proteins from complex biological fluids and analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS). Polylysine-coated diamond is used for solid-phase extraction to pre-concentrate DNA oligonucleotides. Graphite is useful for desalting, pre-concentration, and as energy-absorbing material (matrix) in desorption/ionization. Carbon nano-tubes in their different derivatized forms are used as matrix materials for the analysis of a range of analytes, such as carbohydrates, amino acids, peptides, proteins, and some environmental samples by LDI-MS. Fullerenes are modified in different ways to bind serum entities analyzed through MALDI/TOF-MS and are subsequently utilized in their identifications. In addition, the fullerenes are a promising matrix in LDI-MS, but improvements are needed.

[1]  Koichi Tanaka,et al.  Protein and polymer analyses up to m/z 100 000 by laser ionization time-of-flight mass spectrometry , 1988 .

[2]  Yinlong Guo,et al.  Oxidized carbon nanotubes as matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of biomolecules. , 2005, Rapid communications in mass spectrometry : RCM.

[3]  G. Bonn,et al.  Cu(II)-loaded iminodiacetic acid-silica particles for protein profiling of human serum samples using surface-enhanced affinity capture: support porosity effects. , 2005, Rapid communications in mass spectrometry : RCM.

[4]  R. Zenobi,et al.  Artificial Photoaging of Triterpenes Studied by Graphite‐Assisted Laser Desorption/Ionization Mass Spectrometry , 2000 .

[5]  J. Sunner,et al.  An activated carbon substrate surface for laser desorption mass spectrometry , 2000, Journal of the American Society for Mass Spectrometry.

[6]  C. Huck,et al.  Nanocrystalline diamond--an excellent platform for life science applications. , 2007, Journal of nanoscience and nanotechnology.

[7]  G. Jiang,et al.  Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with a matrix of carbon nanotubes for the analysis of low-mass compounds in environmental samples. , 2005, Environmental science & technology.

[8]  Jeffrey C. Smith,et al.  Collisional cooling enhances the ability to observe non-covalent interactions within the inducible nitric oxide synthase oxygenase domain: Dimerization, complexation, and dissociation , 2004, Journal of the American Society for Mass Spectrometry.

[9]  Thomas Franz,et al.  Silicone/graphite coating for on‐target desalting and improved peptide mapping performance of matrix‐assisted laser desorption/ionization‐mass spectrometry targets in proteomic experiments , 2005, Proteomics.

[10]  H. Zou,et al.  Separation and detection of compounds in Honeysuckle by integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analy , 2006, Journal of pharmaceutical and biomedical analysis.

[11]  D. Y. Yoon,et al.  Observation of low molecular weight poly(methylsilsesquioxane)s by graphite plate laser desorption/ionization time-of-flight mass spectrometry. , 2000, Analytical chemistry.

[12]  Chau-Chung Han,et al.  Polylysine-coated diamond nanocrystals for MALDI-TOF mass analysis of DNA oligonucleotides. , 2005, Analytical chemistry.

[13]  R. Zenobi,et al.  Graphite/Liquid mixed matrices for laser desorption/ionization mass spectrometry. , 1996, Analytical chemistry.

[14]  J. A. Schultz,et al.  MALDI matrices for biomolecular analysis based on functionalized carbon nanomaterials. , 2004, Analytical chemistry.

[15]  H. Benyamini,et al.  Formation and characterization of stable human serum albumin-tris-malonic acid [C60]fullerene complex. , 2005, Bioconjugate chemistry.

[16]  A. Guttman,et al.  Material-enhanced laser desorption/ionization (MELDI)—A new protein profiling tool utilizing specific carrier materials for time of flight mass spectrometric analysis , 2006, Journal of the American Society for Mass Spectrometry.

[17]  Yu-Chie Chen,et al.  Carbon nanotubes as affinity probes for peptides and proteins in MALDI MS analysis , 2004, Journal of the American Society for Mass Spectrometry.

[18]  Yu Zhang,et al.  Using oxidized carbon nanotubes as matrix for analysis of small molecules by MALDI-TOF MS , 2005, Journal of the American Society for Mass Spectrometry.

[19]  Xueguo Chen,et al.  Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea cor , 2005, Journal of chromatography. A.

[20]  G. Bonn,et al.  Characterisation and evaluation of metal-loaded iminodiacetic acid-silica of different porosity for the selective enrichment of phosphopeptides. , 2005, Journal of chromatography. A.

[21]  Hanfa Zou,et al.  Carbon nanotubes as assisted matrix for laser desorption/ionization time-of-flight mass spectrometry. , 2003, Analytical chemistry.

[22]  J. Sunner,et al.  Graphite surface-assisted laser desorption/ionization time-of-flight mass spectrometry of peptides and proteins from liquid solutions. , 1995, Analytical chemistry.

[23]  M. Karas,et al.  Novel method for matrix-assisted laser mass spectrometry of proteins , 1991 .

[24]  Yu-Chie Chen,et al.  A novel approach of combining thin-layer chromatography with surface-assisted laser desorption/ionization (SALDI) time-of-flight mass spectrometry. , 2002, Journal of mass spectrometry : JMS.

[25]  Peter Dunnill,et al.  Non-porous magnetic chelator supports for protein recovery by immobilised metal affinity adsorption , 1996 .

[26]  Jen-Pan Huang,et al.  Use of a water-soluble fullerene derivative as precipitating reagent and matrix-assisted laser desorption/ionization matrix to selectively detect charged species in aqueous solutions. , 2003, Analytical chemistry.

[27]  A new analytical material-enhanced laser desorption ionization (MELDI) based approach for the determination of low-mass serum constituents using fullerene derivatives for selective enrichment. , 2007, Journal of proteome research.

[28]  Sang-Won Lee,et al.  Enrichment of N-terminal sulfonated peptides by a water-soluble fullerene derivative and its applications to highly efficient proteomics. , 2006, Analytica chimica acta.

[29]  Li Zhang,et al.  Immobilized carbon nanotubes as matrix for MALDI-TOF-MS analysis: Applications to neutral small carbohydrates , 2005, Journal of the American Society for Mass Spectrometry.

[30]  C. Huck,et al.  Ultra-fast mass fingerprinting by high-affinity capture of peptides and proteins on derivatized poly(glycidyl methacrylate/divinylbenzene) for the analysis of serum and cell lysates. , 2006, Rapid communications in mass spectrometry : RCM.

[31]  H. Zou,et al.  Carbon nanotubes as adsorbent of solid-phase extraction and matrix for laser desorption/ionization mass spectrometry , 2005, Journal of the American Society for Mass Spectrometry.

[32]  A. Hirsch,et al.  Fullerene Chemistry in Three Dimensions: Isolation of Seven Regioisomeric Bisadducts and Chiral Trisadducts of C60 and Di(ethoxycarbonyl)methylene , 1994 .

[33]  H. Klocker,et al.  Derivatized cellulose combined with MALDI-TOF MS: a new tool for serum protein profiling. , 2005, Journal of proteome research.

[34]  M. S. de Vries,et al.  Cobalt-catalysed growth of carbon nanotubes with single-atomic-layer walls , 1993, Nature.

[35]  F. Dubois,et al.  A graphite-assisted laser desorption/ionization study of light-induced aging in triterpene dammar and mastic varnishes , 1998 .

[36]  S. Iijima.  Helical microtubules of graphitic carbon , 1991, Nature.

[37]  C. Huck,et al.  Chemically Modified Nano Crystalline Diamond Layer as Material Enhanced Laser Desorption Ionisation (MELDI) Surface in Protein Profiling , 2006 .

[38]  K. Paek,et al.  Visible surface-assisted laser desorption/ionization mass spectrometry of small macromolecules deposited on the graphite plate , 2002 .

[39]  L. Chiang,et al.  Versatile nitronium chemistry for C60 fullerene functionalization , 1992 .

[40]  H-C Chang,et al.  High-affinity capture of proteins by diamond nanoparticles for mass spectrometric analysis. , 2005, Analytical chemistry.

[41]  M. Karas,et al.  Energy deposition in ultraviolet laser desorption mass spectrometry of biomolecules , 1986 .

[42]  M. Karas,et al.  Matrix-assisted ultraviolet laser desorption of non-volatile compounds , 1987 .

[43]  K. Fisher,et al.  C60‐assisted laser desorption/ionization mass spectrometry in the analysis of phosphotungstic acid , 1994 .

[44]  E. Nakamura,et al.  Functionalized fullerenes in water. The first 10 years of their chemistry, biology, and nanoscience. , 2003, Accounts of chemical research.

[45]  Alexandra Shulman-Peleg,et al.  Interaction of c(60)-fullerene and carboxyfullerene with proteins: docking and binding site alignment. , 2006, Bioconjugate chemistry.

[46]  C. Huck,et al.  Chemically modified carbon nanotubes as material enhanced laser desorption ionisation (MELDI) material in protein profiling , 2006 .

[47]  Zhang Jing,et al.  Amino Acids Analysis by MALDI Mass Spectrometry Using Carbon Nanotube as Matrix , 2005 .

[48]  P. Roepstorff,et al.  Graphite powder as an alternative or supplement to reversed‐phase material for desalting and concentration of peptide mixtures prior to matrix‐assisted laser desorption/ionization‐mass spectrometry , 2002, Proteomics.

[49]  Ronald C. Beavis,et al.  Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers. , 1991, Analytical chemistry.