Nitrate removal in stream ecosystems measured by 15N addition experiments: Denitrification

We measured denitrification rates using a field 15NO3− tracer‐addition approach in a large, cross‐site study of nitrate uptake in reference, agricultural, and suburban‐urban streams. We measured denitrification rates in 49 of 72 streams studied. Uptake length due to denitrification (SWdenn) ranged from 89 m to 184 km (median of 9050 m) and there were no significant differences among regions or land‐use categories, likely because of the wide range of conditions within each region and land use. N2 production rates far exceeded N2O production rates in all streams. The fraction of total NO3− removal from water due to denitrification ranged from 0.5% to 100% among streams (median of 16%), and was related to NH4+ concentration and ecosystem respiration rate (ER). Multivariate approaches showed that the most important factors controlling SWden were specific discharge (discharge / width) and NO3− concentration (positive effects), and ER and transient storage zones (negative effects). The relationship between areal denitrification rate (Uden) and NO3− concentration indicated a partial saturation effect. A power function with an exponent of 0.5 described this relationship better than a Michaelis‐Menten equation. Although Uden increased with increasing NO3− concentration, the efficiency of NO3− removal from water via denitrification declined, resulting in a smaller proportion of streamwater NO3− load removed over a given length of stream. Regional differences in stream denitrification rates were small relative to the proximate factors of NO3− concentration and ecosystem respiration rate, and land use was an important but indirect control on denitrification in streams, primarily via its effect on NO3− concentration.

[1]  William H. McDowell,et al.  Nitrate removal in stream ecosystems measured by 15N addition experiments: Total uptake , 2009 .

[2]  Mark B. David,et al.  Dynamic modeling of nitrogen losses in river networks unravels the coupled effects of hydrological and biogeochemical processes , 2009 .

[3]  William H. McDowell,et al.  Stream denitrification across biomes and its response to anthropogenic nitrate loading , 2008, Nature.

[4]  J. Tank,et al.  Land use influences the spatiotemporal controls on nitrification and denitrification in headwater streams , 2008, Journal of the North American Benthological Society.

[5]  M. Alber,et al.  Temperature controls a latitudinal gradient in the proportion of watershed nitrogen exported to coastal ecosystems , 2007 .

[6]  S. Hamilton,et al.  Measurement of the stable isotope ratio of dissolved N2 in 15N tracer experiments , 2007 .

[7]  W. Keeton,et al.  Mature and old-growth riparian forests: structure, dynamics, and effects on Adirondack stream habitats. , 2007, Ecological applications : a publication of the Ecological Society of America.

[8]  Walter K. Dodds,et al.  The saturation of N cycling in Central Plains streams: 15N experiments across a broad gradient of nitrate concentrations , 2007 .

[9]  F. Bormann,et al.  Concepts and Methods for Assessing Solute Dynamics in Stream Ecosystems , 2007 .

[10]  J A Harrison,et al.  Denitrification across landscapes and waterscapes: a synthesis. , 2006, Ecological applications : a publication of the Ecological Society of America.

[11]  M. Álvarez-Cobelas,et al.  Denitrification in Aquatic Environments: A Cross-system Analysis , 2006 .

[12]  John Fox,et al.  TEACHER'S CORNER: Structural Equation Modeling With the sem Package in R , 2006 .

[13]  S. Seitzinger,et al.  Diurnal patterns of denitrification, oxygen consumption and nitrous oxide production in rivers measured at the whole‐reach scale , 2004 .

[14]  G. Asner,et al.  Nitrogen Cycles: Past, Present, and Future , 2004 .

[15]  T. Royer,et al.  Transport and fate of nitrate in headwater agricultural streams in Illinois. , 2004, Journal of environmental quality.

[16]  J. Böhlke,et al.  Reach‐scale isotope tracer experiment to quantify denitrification and related processes in a nitrate‐rich stream, midcontinent United States , 2004 .

[17]  Sybil P. Seitzinger,et al.  Measurement of denitrification in rivers: an integrated, whole reach approach , 2002, Hydrobiologia.

[18]  W. Dodds,et al.  Nitrogen Retention, Removal, and Saturation in Lotic Ecosystems , 2005, Ecosystems.

[19]  Wayne S Gardner,et al.  Comparing Denitrification Estimates for a Texas Estuary by Using Acetylene Inhibition and Membrane Inlet Mass Spectrometry , 2003, Applied and Environmental Microbiology.

[20]  M. Peruggia.  Model Selection and Multimodel Inference: A Practical Information-Theoretic Approach (2nd ed.) , 2003 .

[21]  David R. Anderson,et al.  Model selection and multimodel inference : a practical information-theoretic approach , 2003 .

[22]  W. Lewis,et al.  Open‐channel estimation of denitrification , 2003 .

[23]  Robert L. Runkel,et al.  A new metric for determining the importance of transient storage , 2002, Journal of the North American Benthological Society.

[24]  W. Dodds,et al.  The influence of ammonium, nitrate, and dissolved oxygen concentrations on uptake, nitrification, and denitrification rates associated with prairie stream substrata , 2002 .

[25]  S. Seitzinger,et al.  The role of denitrification in nitrogen removal and carbon mineralization in Mid-Atlantic Bight sediments , 2002 .

[26]  Elizabeth W. Boyer,et al.  Anthropogenic nitrogen sources and relationships to riverine nitrogen export in the northeastern U.S.A. , 2002 .

[27]  Eileen H. Helmer,et al.  Mapping the Forest Type and Land Cover of Puerto Rico, a Component of the Caribbean Biodiversity Hotspot , 2002 .

[28]  S. Hamilton,et al.  Control of Nitrogen Export from Watersheds by Headwater Streams , 2001, Science.

[29]  J. Burkholder,et al.  WATER QUALITY TRENDS AND MANAGEMENT IMPLICATIONS FROM A FIVE-YEAR STUDY OF A EUTROPHIC ESTUARY , 2000 .

[30]  Gregory E. Schwarz,et al.  Effect of stream channel size on the delivery of nitrogen to the Gulf of Mexico , 2000, Nature.

[31]  B. Shipley.  Cause and correlation in biology , 2000 .

[32]  R. Freckleton,et al.  The Ecological Detective: Confronting Models with Data , 1999 .

[33]  Awwa,et al.  Standard Methods for the examination of water and wastewater , 1999 .

[34]  Alan D. Steinman,et al.  Improvements to the diurnal upstream-downstream dissolved oxygen change technique for determining whole-stream metabolism in small streams , 1998 .

[35]  Pattinson,et al.  Denitrification in river sediments: relationship between process rate and properties of water and sediment , 1998 .

[36]  J. Cornwell,et al.  Denitrification in estuarine sediments determined by membrane inlet mass spectrometry , 1998 .

[37]  Robert L. Runkel,et al.  One-Dimensional Transport with Inflow and Storage (OTIS): A Solute Transport Model for Streams and Rivers , 1998 .

[38]  G. Likens,et al.  Technical Report: Human Alteration of the Global Nitrogen Cycle: Sources and Consequences , 1997 .

[39]  R. Michener,et al.  Natural abundance-level measurement of the nitrogen isotopic composition of oceanic nitrate: an adaptation of the ammonia diffusion method , 1997 .

[40]  Donald E. Weller,et al.  Human contributions to terrestrial nitrogen flux , 1996 .

[41]  F. Triska,et al.  Nitrate reduction in sediments of lowland tropical streams draining swamp forest in Costa Rica: An ecosystem perspective , 1996 .

[42]  Jeremy B. Jones,et al.  Denitrification in a nitrogen-limited stream ecosystem , 1996 .

[43]  Dennis P. Swaney,et al.  Regional nitrogen budgets and riverine N & P fluxes for the drainages to the North Atlantic Ocean: Natural and human influences , 1996 .

[44]  N. Risgaard-Petersen,et al.  Oxygen regulation of nitrification and denitrification in sediments , 1994 .

[45]  L. Nielsen,et al.  Diurnal variation of denitrification and nitrification in sediments colonized by benthic microphytes , 1994 .

[46]  N. Rabalais,et al.  LSU Digital Commons LSU Digital Commons Coastal Eutrophication near the Mississippi River Delta Coastal Eutrophication near the Mississippi River Delta , 2022 .

[47]  T. Blackburn,et al.  Effect of organic loading on nitrification and denitrification in a marine sediment microcosm , 1993 .

[48]  F. Triska,et al.  Patterns of hydrological exchange and nutrient transformation in the hyporheic zone of a gravel‐bottom stream: examining terrestrial—aquatic linkages , 1993 .

[49]  F. Triska,et al.  Denitrification in sediments from the hyporheic zone adjacent to a small forested stream , 1990 .

[50]  J. Sørensen,et al.  Denitrification in nitrate-rich streams: Diurnal and seasonal variation related to benthic oxygen metabolism , 1990 .

[51]  Nicholas G. Aumen,et al.  Concepts and methods for assessing solute dynamics in stream ecosystems , 1990 .

[52]  S. Seitzinger.  Denitrification in freshwater and coastal marine ecosystems: Ecological and geochemical significance , 1988 .

[53]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[54]  Roy A. Walters,et al.  Simulation of solute transport in a mountain pool‐and‐riffle stream: A transient storage model , 1983 .

[55]  R. O'Neill,et al.  Measuring Nutrient Spiralling in Streams , 1981 .

[56]  H. Akaike.  INFORMATION THEORY AS AN EXTENSION OF THE MAXIMUM LIKELIHOOD , 1973 .

[57]  H. Akaike,et al.  Information Theory and an Extension of the Maximum Likelihood Principle , 1973 .

[58]  H. Odum.  Primary Production in Flowing Waters1 , 1956 .