Serological and molecular identification of subclinical chicken anaemia virus infection in broiler chickens in Iran

Chicken anaemia virus (CAV) can occur in immunosuppressive syndrome in broiler chickens. CAV infection in presence of maternal antibody appears sub-clinically. In this study subclinical infection of CAV was studied in broiler chickens in four provinces of Iran, by ELISA and PCR. For this, total of 375 serum samples and 375 thymus samples were collected from 25 broiler chicken flocks. Results showed all flocks to be positive by ELISA and PCR (in which at least one chicken was detected as positive). The positivity rate of CAV infection in broiler chickens, following PCR assay, was 58.4%. The results indicate high prevalence of subclinical CAV infection in Iran. Therefore, a proper vaccination strategy in broiler breeder and even broiler chickens, without interfering with maternal antibodies, must be investigated.   Key words: Chicken anaemia virus, subclinical, Iran

[1]  O. Erganiş,et al.  Investigation of Chicken Infectious Anemia Virus Infection by PCR and ELISA in Chicken Flocks , 2008 .

[2]  N. Bhardwaj,et al.  Biological and molecular characterization of chicken anaemia virus isolates of Indian origin. , 2006, Virus research.

[3]  A. Roussan.  Serological Survey on the Prevalence of Chicken Infectious Anemia Virus in Commercial Broiler Chicken Flocks in Northern Jordan , 2006 .

[4]  I. Karimi,et al.  Serologic Evidence of Chicken Infectious Anemia in Commercial Chicken Flocks in Shahrekord, Iran , 2005 .

[5]  K. Schat,et al.  Chicken Infectious Anemia Virus: An Example of the Ultimate Host–Parasite Relationship , 2004, Avian diseases.

[6]  C. Cardona,et al.  Chicken Anemia Virus in Broilers: Dynamics of the Infection in Two Commercial Broiler Flocks , 2003, Avian diseases.

[7]  C. Cardona,et al.  Humoral immune responses to chicken infectious anemia virus in three strains of chickens in a closed flock. , 2000, Avian diseases.

[8]  B. Adair.  Immunopathogenesis of chicken anemia virus infection. , 2000, Developmental and comparative immunology.

[9]  T. Hayashi,et al.  A serological survey of chickens, Japanese quail, pigeons, ducks and crows for antibodies to chicken anaemia virus (CAV) in Japan. , 1998, Avian pathology : journal of the W.V.P.A.

[10]  G. Koch,et al.  Interaction between chicken anaemia virus and live Newcastle disease vaccine. , 1994, Avian pathology : journal of the W.V.P.A.

[11]  W. Stanislawek,et al.  Polymerase chain reaction amplification for direct detection of chicken anemia virus DNA in tissues and sera. , 1992, Avian diseases.

[12]  R. Hoop.  Persistence and vertical transmission of chicken anaemia agent in experimentally infected laying hens. , 1992, Avian pathology : journal of the W.V.P.A.

[13]  E. Goodall,et al.  Economic effects of clinical chicken anemia agent infection on profitable broiler production. , 1992, Avian diseases.

[14]  M. Mcnulty,et al.  Economic effects of subclinical chicken anemia agent infection in broiler chickens. , 1991, Avian diseases.

[15]  F. Rixon,et al.  Purification and biochemical characterization of chicken anaemia agent. , 1990, The Journal of general virology.

[16]  S. Lister,et al.  An outbreak of disease due to chicken anaemia agent in broiler chickens in England , 1989, Veterinary Record.

[17]  F. McNeilly,et al.  A serological survey of domestic poultry in the United Kingdom for antibody to chicken anaemia agent. , 1988, Avian pathology : journal of the W.V.P.A.

[18]  T. Nunoya,et al.  Depression of vaccinal immunity to Marek's disease by infection with chicken anaemia agent. , 1988, Avian pathology : journal of the W.V.P.A.

[19]  K. Imai,et al.  Aetiological examination of an outbreak of haemorrhagic syndrome in a broiler flock in Japan. , 1987, Avian pathology : journal of the W.V.P.A.