Saffron Samples of Different Origin: An NMR Study of Microwave-Assisted Extracts

An NMR analytical protocol is proposed to characterize saffron samples of different geographical origin (Greece, Spain, Hungary, Turkey and Italy). A microwave-assisted extraction procedure was developed to obtain a comparable recovery of metabolites with respect to the ISO specifications, reducing the solvent volume and the extraction time needed. Metabolite profiles of geographically different saffron extracts were compared showing significant differences in the content of some metabolites.

[1]  S. Braun,et al.  100 and more basic NMR experiments , 1996 .

[2]  Javad Asili,et al.  1H NMR metabolic fingerprinting of saffron extracts , 2010, Metabolomics.

[3]  Luisa Mannina,et al.  Liquid state 1H high field NMR in food analysis. , 2012, Progress in nuclear magnetic resonance spectroscopy.

[4]  H. Hosseinzadeh,et al.  Clinical Applications of Saffron (Crocus sativus) and its Constituents: A Review , 2014, Drug Research.

[5]  Rogelio Pereda-Miranda,et al.  HPLC quantification of major active components from 11 different saffron (Crocus sativus L.) sources , 2007 .

[6]  S. Carradori,et al.  NMR methodologies in the analysis of blueberries , 2014, Electrophoresis.

[7]  Ying Wang,et al.  Microwave assisted extraction of secondary metabolites from plants: Current status and future directions , 2011 .

[8]  Miguel Valcárcel,et al.  Determination of total safranal by in situ acid hydrolysis in supercritical fluid media: Application to the quality control of commercial saffron. , 2006, Analytica chimica acta.

[9]  A. Tomassini,et al.  Monitoring of metabolic profiling and water status of Hayward kiwifruits by nuclear magnetic resonance. , 2010, Talanta.

[10]  S. Engelsen,et al.  Metabolic profiling and aquaculture differentiation of gilthead sea bream by 1H NMR metabonomics. , 2010 .

[11]  J. Beauchamp,et al.  International Organization for Standardization (ISO) , 2015 .

[12]  L. Mannina,et al.  High resolution NMR characterization of olive oils in terms of quality, authenticity and geographical origin , 2011, Magnetic resonance in chemistry : MRC.

[13]  D. Capitani,et al.  NMR metabolic profiling of organic and aqueous sea bass extracts: implications in the discrimination of wild and cultured sea bass. , 2008, Talanta.

[14]  S. C. Kim,et al.  Proposed cytotoxic mechanisms of the saffron carotenoids crocin and crocetin on cancer cell lines. , 2014, Biochemistry and cell biology = Biochimie et biologie cellulaire.

[15]  M. Bissonnette,et al.  Spectroscopic Characterization of Crocetin Derivatives from Crocus sativus and Gardenia jasminoides , 1997 .

[16]  L. Mannina,et al.  High-field nuclear magnetic resonance (NMR) study of truffles (Tuber aestivum vittadini). , 2004, Journal of agricultural and food chemistry.

[17]  Charles S. Johnson Diffusion Ordered Nuclear Magnetic Resonance Spectroscopy: Principles and Applications , 1999 .

[18]  G. Allmaier,et al.  Comparison of different tandem mass spectrometric techniques (ESI-IT, ESI- and IP-MALDI-QRTOF and vMALDI-TOF/RTOF) for the analysis of crocins and picrocrocin from the stigmas of Crocus sativus L. , 2012, Rapid communications in mass spectrometry : RCM.

[19]  Gonzalo L. Alonso,et al.  Worldwide market screening of saffron volatile composition , 2009 .

[20]  C. De Monte,et al.  New insights into the biological properties of Crocus sativus L.: chemical modifications, human monoamine oxidases inhibition and molecular modeling studies. , 2014, European journal of medicinal chemistry.

[21]  Peter Winterhalter,et al.  Identification of Novel Glycosidic Aroma Precursors in Saffron (Crocus sativus L.) , 1998 .

[22]  Á. Ríos,et al.  Use of non-aqueous capillary electrophoresis for the quality control of commercial saffron samples. , 2005, Journal of chromatography. A.

[23]  P. Tarantilis,et al.  UV-Vis, FT-Raman, and 1H NMR Spectroscopies of cis-trans Carotenoids from Saffron (Crocus sativus L.) , 1998 .

[24]  S. Bisti,et al.  Saffron and retina: Neuroprotection and pharmacokinetics , 2014, Visual Neuroscience.

[25]  A. Tomassini,et al.  Peach fruit: metabolic comparative analysis of two varieties with different resistances to insect attacks by NMR spectroscopy. , 2013, Journal of agricultural and food chemistry.

[26]  S. Samarghandian,et al.  Anticarcinogenic effect of saffron (Crocus sativus L.) and its ingredients , 2014, Pharmacognosy research.

[27]  Charles L. Cantrell,et al.  Quantification of saffron (Crocus sativus L.) metabolites crocins, picrocrocin and safranal for quality determination of the spice grown under different environmental Moroccan conditions , 2009 .

[28]  Manuel Carmona,et al.  Geographical origin differentiation of saffron spice (Crocus sativus L. stigmas) - Preliminary investigation using chemical and multi-element (H, C, N) stable isotope analysis. , 2011, Food chemistry.

[29]  Massimo F. Marcone,et al.  Chemical and biological properties of the world's most expensive spice: Saffron , 2010 .

[30]  Amaya Zalacain,et al.  Near-infrared spectroscopy in saffron quality control: determination of chemical composition and geographical origin. , 2005, Journal of agricultural and food chemistry.