Comparison of gassing-out and pressure-step dynamic methods for kLa measurement in an airlift reactor with internal loop

Volumetric oxygen mass transfer coefficient (kLa) in an airlift reactor with internal loop (working volume of 40 dm3) was studied experimentally in the water batch. The kLa values were measured using both the dynamic pressure-step method (DPM) proposed by Linek and gassing-out method (GOM), the standard variation. The kLa values obtained by the DPM were not dependent on the concentration of oxygen in the inlet gas and by about 15% higher than those obtained by the GOM. Based on our observations, empirical parameter values in the correlation between kLa and gas holdup, proposed by Bello, were changed to new values.

[1]  J. Prüss,et al.  Scale-up of airlift-loop bioreactors based on modelling the oxygen mass transfer , 1992 .

[2]  V. Linek,et al.  Measurement of kLa in multiple-impeller vessel with significant axial dispersion in both phases , 1995 .

[3]  Determination methods of the volumetric oxygen transfer coefficient kLa in bioreactors , 1990 .

[4]  P. Beneš,et al.  Non-ideal pressure step method for kLa measurement , 1993 .

[5]  Václav Linek,et al.  Gas-liquid mass transfer in vessels stirred with multiple impellers—I. Gas-liquid mass transfer characteristics in individual stages , 1996 .

[6]  V. Linek,et al.  Hydrodynamic and mass transfer characteristics of ejector loop reactors , 2000 .

[7]  V. Linek,et al.  Critical assessment of the steady‐state Na2SO3 feeding method for kla measurement in fermentors , 1990, Biotechnology and bioengineering.

[8]  Václav Linek,et al.  Gas hold-up, mixing time and gas-liquid volumetric mass transfer coefficient of various multiple-impeller configurations: Rushton turbine, pitched blade and techmix impeller and their combinations , 2003 .

[9]  F. Potůček,et al.  Oxygen transfer in liquids , 1985, Biotechnology and bioengineering.

[10]  D. R. Skidmore,et al.  Mass transfer phenomena in an airlift reactor: Effects of solids loading and temperature , 1990, Biotechnology and bioengineering.

[11]  J. Merchuk,et al.  On the first-order approximation to the response of dissolved oxygen electrodes for dynamic KLa estimation. , 1990, Biotechnology and bioengineering.

[12]  P. Beneš,et al.  Dynamic pressure method for kla measurement in large‐scale bioreactors , 1989, Biotechnology and bioengineering.

[13]  P. Gogate,et al.  Survey of measurement techniques for gas–liquid mass transfer coefficient in bioreactors , 1999 .

[14]  Measurement of oxygen by membrane-covered probes : Horwood, Chichester, 1988 (ISBN 0-7458-0199-4). 330 pp. Price £45.00 , 1989 .

[15]  F. Yoshida,et al.  Transient characteristics of oxygen probes and determination of k(L)a. , 1983, Biotechnology and bioengineering.

[16]  V. Linek,et al.  Supersaturation effect in steady-state and dynamic methods for measuring kLa in gas-liquid dispersions , 1996 .

[17]  P. Beneš,et al.  A critical review and experimental verification of the correct use of the dynamic method for the determination of oxygen transfer in aerated agitated vessels to water, electrolyte solutions and viscous liquids , 1987 .

[18]  Hirokazu Kawabata,et al.  GAS HOLDUP AND VOLUMETRIC LIQUID-PHASE MASS TRANSFER COEFFICIENT IN A GEL-PARTICLE SUSPENDED BUBBLE COLUMN WITH DRAUGHT TUBE , 1985 .

[19]  E. S. Gaddis Mass transfer in gas–liquid contactors , 1999 .

[20]  Porntip Wongsuchoto,et al.  Bubble size distribution and gas–liquid mass transfer in airlift contactors , 2003 .

[21]  Yusuf Chisti,et al.  Oxygen transfer and mixing in mechanically agitated airlift bioreactors , 2002 .

[22]  V. Linek,et al.  Measurement of kLa by dynamic pressure method in pilot‐plant fermentor , 1994, Biotechnology and Bioengineering.

[23]  V. Linek,et al.  Critical assessment of gassing‐in methods for measuring kla in fermentors , 1991, Biotechnology and bioengineering.

[24]  Measurement of oxygen by membrane-covered probes : guidelines for applications in chemical and biochemical engineering , 1988 .

[25]  J. Teixeira,et al.  Oxygen mass transfer in a high solids loading three-phase internal-loop airlift reactor , 2001 .

[26]  Campbell W. Robinson,et al.  Prediction of the volumetric mass transfer coefficient in pneumatic contactors , 1985 .