General Methods for Characterization of Brain Regions

The recognition of the components of the central nervous system is directly related to questions concerning the interconnections of neuronal systems within the brain. Therefore, it is necessary to combine a description of structural details with their exact topographical orientation with respect to other structures. Thus, an analysis of neuronal systems requires a profound knowledge of the localization of the smallest areas. It necessitates a specific preparatory method in order to investigate distinct areas by light and electron microscopic means.