Erythrocyte-derived nano-probes functionalized with antibodies for targeted near infrared fluorescence imaging of cancer cells.

Constructs derived from mammalian cells are emerging as a new generation of nano-scale platforms for clinical imaging applications. Herein, we report successful engineering of hybrid nano-structures composed of erythrocyte-derived membranes doped with FDA-approved near infrared (NIR) chromophore, indocyanine green (ICG), and surface-functionalized with antibodies to achieve molecular targeting. We demonstrate that these constructs can be used for targeted imaging of cancer cells in vitro. These erythrocyte-derived optical nano-probes may provide a potential platform for clinical translation, and enable molecular imaging of cancer biomarkers.

[1]  V Ntziachristos,et al.  Intraoperative near-infrared fluorescence imaging for sentinel lymph node detection in vulvar cancer: first clinical results. , 2011, Gynecologic oncology.

[2]  T. Toyota,et al.  Near-infrared-fluorescence imaging of lymph nodes by using liposomally formulated indocyanine green derivatives. , 2014, Bioorganic & medicinal chemistry.

[3]  A. Vahrmeijer,et al.  Dose optimization for near‐infrared fluorescence sentinel lymph node mapping in patients with melanoma , 2013, The British journal of dermatology.

[4]  A. Welch,et al.  A review of the optical properties of biological tissues , 1990 .

[5]  Bahman Anvari,et al.  Erythrocyte-derived photo-theranostic agents: hybrid nano-vesicles containing indocyanine green for near infrared imaging and therapeutic applications , 2013, Scientific Reports.

[6]  Dennis E Discher,et al.  Minimal " Self " Peptides That Inhibit Phagocytic Clearance and Enhance Delivery of Nanoparticles References and Notes , 2022 .

[7]  B. Liu,et al.  Hybrid polypeptide micelles loading indocyanine green for tumor imaging and photothermal effect study. , 2013, Biomacromolecules.

[8]  C. Pirich,et al.  Visualisation of the lymph node pathway in real time by laparoscopic radioisotope- and fluorescence-guided sentinel lymph node dissection in prostate cancer staging. , 2012, Urology.

[9]  E. Scott,et al.  Gadolinium-doped silica nanoparticles encapsulating indocyanine green for near infrared and magnetic resonance imaging. , 2012, Small.

[10]  Vladimir Tolmachev,et al.  Optimal specific radioactivity of anti-HER2 Affibody molecules enables discrimination between xenografts with high and low HER2 expression levels , 2011, European Journal of Nuclear Medicine and Molecular Imaging.

[11]  Liangfang Zhang,et al.  Erythrocyte‐Inspired Delivery Systems , 2012, Advanced healthcare materials.

[12]  R. Spetzler,et al.  Indocyanine Green Angiography in the Surgical Management of Cerebral Arteriovenous Malformations: Lessons Learned in 130 Consecutive Cases , 2014, Neurosurgery.

[13]  B. Bax,et al.  Survival of human carrier erythrocytes in vivo. , 1999, Clinical science.

[14]  A. Santin,et al.  HER2 Expression Beyond Breast Cancer: Therapeutic Implications for Gynecologic Malignancies , 2013, Molecular Diagnosis & Therapy.

[15]  Ronnie H. Fang,et al.  Lipid-insertion enables targeting functionalization of erythrocyte membrane-cloaked nanoparticles. , 2013, Nanoscale.

[16]  Sheng-Wen Huang,et al.  Indocyanine-green-embedded PEBBLEs as a contrast agent for photoacoustic imaging. , 2007, Journal of biomedical optics.

[17]  Josephine C. Adams,et al.  The evolution of thrombospondins and their ligand-binding activities. , 2010, Molecular biology and evolution.

[18]  P. Oldenborg.  CD47: A Cell Surface Glycoprotein Which Regulates Multiple Functions of Hematopoietic Cells in Health and Disease , 2013, ISRN hematology.

[19]  O. Ganslandt,et al.  Essentials in intraoperative indocyanine green videoangiography assessment for intracranial aneurysm surgery: conclusions from 295 consecutively clipped aneurysms and review of the literature. , 2014, Neurosurgical focus.

[20]  R. Weissleder,et al.  Near infrared fluorescence-based bacteriophage particles for ratiometric pH imaging. , 2008, Bioconjugate chemistry.

[21]  Bahman Anvari,et al.  Effects of nanoencapsulation and PEGylation on biodistribution of indocyanine green in healthy mice: quantitative fluorescence imaging and analysis of organs , 2013, International journal of nanomedicine.

[22]  M. Epple,et al.  Gold nanoparticles: dispersibility in biological media and cell-biological effect , 2010 .

[23]  Antonella Antonelli,et al.  Red blood cells as carriers of iron oxide-based contrast agents for diagnostic applications. , 2014, Journal of biomedical nanotechnology.

[24]  J. M. Lanao,et al.  Cell-based drug-delivery platforms. , 2012, Therapeutic delivery.

[25]  W. Liu,et al.  Red Blood Cell Membrane as a Biomimetic Nanocoating for Prolonged Circulation Time and Reduced Accelerated Blood Clearance. , 2015, Small.

[26]  J. Frangioni.  In vivo near-infrared fluorescence imaging. , 2003, Current opinion in chemical biology.

[27]  B Gleich,et al.  Nanoparticle encapsulation in red blood cells enables blood-pool magnetic particle imaging hours after injection , 2013, Physics in medicine and biology.

[28]  J. C. Kraft,et al.  Interactions of Indocyanine Green and Lipid in Enhancing Near-Infrared Fluorescence Properties: The Basis for Near-Infrared Imaging in Vivo , 2014, Biochemistry.

[29]  Vladimir R Muzykantov,et al.  Drug delivery by red blood cells: vascular carriers designed by mother nature , 2010, Expert opinion on drug delivery.

[30]  C. Lagenaur,et al.  Role of CD47 as a marker of self on red blood cells. , 2000, Science.

[31]  L. Yannuzzi,et al.  Indocyanine green angiography: a perspective on use in the clinical setting. , 2011, American journal of ophthalmology.

[32]  H. Kantarjian,et al.  Red blood cell-encapsulated L-asparaginase: potential therapy of patients with asparagine synthetase deficient acute myeloid leukemia. , 2013, Protein and peptide letters.

[33]  Omid C Farokhzad,et al.  Self-assembled targeted nanoparticles: evolution of technologies and bench to bedside translation. , 2011, Accounts of chemical research.

[34]  N. Gjerdet,et al.  Agglomeration and sedimentation of TiO2 nanoparticles in cell culture medium. , 2009, Colloids and surfaces. B, Biointerfaces.

[35]  Michael Hünerbein,et al.  ICG fluorescence-guided sentinel node biopsy for axillary nodal staging in breast cancer , 2010, Breast Cancer Research and Treatment.

[36]  María Vallet-Regí,et al.  The influence of proteins on the dispersability and cell-biological activity of silver nanoparticles , 2010 .

[37]  Gianluca Damonte,et al.  Erythrocyte-Mediated Delivery of Dexamethasone in Patients With Mild-to-Moderate Ulcerative Colitis, Refractory to Mesalamine: A Randomized, Controlled Study , 2008, The American Journal of Gastroenterology.

[38]  D. Irvine,et al.  Bio-inspired, bioengineered and biomimetic drug delivery carriers , 2011, Nature Reviews Drug Discovery.

[39]  J. Karp,et al.  Nanocarriers as an Emerging Platform for Cancer Therapy , 2022 .

[40]  Milton V. Marshall,et al.  Imaging of lymph flow in breast cancer patients after microdose administration of a near-infrared fluorophore: feasibility study. , 2008, Radiology.

[41]  R. Flower,et al.  Observation of erythrocyte dynamics in the retinal capillaries and choriocapillaris using ICG-loaded erythrocyte ghost cells. , 2008, Investigative ophthalmology & visual science.

[42]  R. Kurzrock,et al.  HER2 aberrations in cancer: implications for therapy. , 2014, Cancer treatment reviews.

[43]  Bahman Anvari,et al.  Stability assessment of indocyanine green within dextran-coated mesocapsules by absorbance spectroscopy. , 2007, Journal of biomedical optics.

[44]  James H. Adair,et al.  Calcium phosphate nanocomposite particles for in vitro imaging and encapsulated chemotherapeutic drug delivery to cancer cells. , 2008, Nano letters.

[45]  S. Guichard,et al.  Selective inhibition of HER2 inhibits AKT signal transduction and prolongs disease-free survival in a micrometastasis model of ovarian carcinoma. , 2005, Annals of oncology : official journal of the European Society for Medical Oncology.

[46]  Ronnie H. Fang,et al.  Erythrocyte membrane-camouflaged polymeric nanoparticles as a biomimetic delivery platform , 2011, Proceedings of the National Academy of Sciences.

[47]  Omid C Farokhzad,et al.  Targeted polymeric therapeutic nanoparticles: design, development and clinical translation. , 2012, Chemical Society reviews.