Naturally-occurring antibodies to poly(ADP-ribose) in patients with systemic lupus erythematosus

POLY(ADP-RIBOSE) is a biopolymer which is synthesised by poly(ADP-ribose) polymerase in cell nuclei1. Its structure and physicochemical characteristics have been reviewed1. Its biological function was suggested to be related to DNA synthesis in nuclei2–4, and there are several reports on its natural occurrence5–9. We reported previously that a specific antibody to poly(ADP-ribose) could be produced in rabbits injected with a complex of poly(ADP-ribose) and methylated bovine serum albumin10. Kidwell and Mage reported on the presence of poly(ADP-ribose) in HeLa cells and the correlation of its content to cell cycles by a radioimmunoassay11. Recently we found that binding activities of the sera of patients with systemic lupus erythematosus (SLE) to 14C-poly(ADP-ribose) were higher than those of the sera of normal individuals, and we suggest that poly(ADP-ribose) as well as antibodies to poly(ADP-ribose) occur naturally in human beings. This paper reports that binding activities of the sera of SLE patients to 14C-poly(ADP-ribose) are attributable to immunoglobulins, mainly to IgG, but not to nonspecific interactions between 14C-poly(ADP-ribose) and IgG; specificity of the binding was verified by inhibition tests using unlabelled poly(ADP-ribose) and 14 related compounds.

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