Synthesis and characterization of waxberry-like microstructures ZnO for biosensors

Abstract The waxberry-like ZnO microstructure composed of 8–10 nm nanorods was synthesized by a novel wet method, and characterized by using X-ray diffractometry (XRD), field emission scanning electron microscopy (FESEM), high-resolution transmission electron microscopy (HRTEM), photoluminescence (PL) spectra, Brunauer–Emmett–Teller (BET) surface area analyses, and FT-IR spectra. In addition, the as-prepared products were co-immobilized with horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode (GCE) to electrochemically study the effect of the novel structure of ZnO on the catalytic activity of HRP. It was found that the waxberry-like ZnO balls acted as excellent materials for immobilization of HRP and rapid electron transfer agent for the fabrication of efficient biosensors due to their high electron conductivity, large surface area, increased oxygen vacancies, and good biocompatibility.

[1]  D. Bertho,et al.  Influence of II–VI nanocrystal shapes on optical properties , 1998 .

[2]  Xu Wu,et al.  Photoluminescence and Optically Pumped Ultraviolet Lasing from Nanocrystalline ZnO Thin Films Prepared by Thermal Oxidation of High-Quality ZnS Thin Films , 2002 .

[3]  Y. Chai,et al.  A mediator-free amperometric hydrogen peroxide biosensor based on HRP immobilized on a nano-Au/poly 2,6-pyridinediamine-coated electrode , 2007, Bioprocess and biosystems engineering.

[4]  S. K. Sadrnezhaad,et al.  Highly textured ZnO thin films: An economical fabrication, doping by Mn2+ and Sn2+ and approachment for optical devices , 2007 .

[5]  Richard Phillips Feynman,et al.  Very high-energy collisions of hadrons , 1969 .

[6]  Douglas J. Moffatt,et al.  Fourier Self-Deconvolution: A Method for Resolving Intrinsically Overlapped Bands , 1981 .

[7]  Electrochemistry and spectroscopy study on the interaction of microperoxidase-11 with lipid membrane. , 2001, Biophysical chemistry.

[8]  N. Serpone,et al.  Kinetics studies in heterogeneous photocatalysis. I. Photocatalytic degradation of chlorinated phenols in aerated aqueous solutions over titania supported on a glass matrix , 1988 .

[9]  Richard A. Mathies,et al.  Size-Controlled Growth of CdSe Nanocrystals in Microfluidic Reactors , 2003 .

[10]  P. Ghosh,et al.  A practical approach to produce near-spherical common salt crystals with better flow characteristics , 2006 .

[11]  Xiao Wei Sun,et al.  Hydrothermally grown oriented ZnO nanorod arrays for gas sensing applications , 2006 .

[12]  William L. Warren,et al.  Correlation between photoluminescence and oxygen vacancies in ZnO phosphors , 1996 .

[13]  Jih-Jen Wu,et al.  Catalyst-Free Growth and Characterization of ZnO Nanorods , 2002 .

[14]  Bin Liu,et al.  Hydrothermal synthesis of ZnO nanorods in the diameter regime of 50 nm. , 2003, Journal of the American Chemical Society.

[15]  M. A. Khadar,et al.  Role of grain boundaries on the electrical conductivity of nanophase zinc oxide , 2001 .

[16]  Sten-Eric Lindquist,et al.  Three-dimensional array of highly oriented crystalline ZnO microtubes , 2001 .

[17]  Zhong Lin Wang,et al.  Catalyst-free MOCVD growth of aligned ZnO nanotip arrays on silicon substrate with controlled tip shape , 2007 .

[18]  Wenqin Peng,et al.  Synthesis and Structures of Morphology-Controlled ZnO Nano- and Microcrystals , 2006 .

[19]  Guo-Li Shen,et al.  A mediator-free phenol biosensor based on immobilizing tyrosinase to ZnO nanoparticles. , 2006, Analytical biochemistry.

[20]  Bixia Lin,et al.  Green luminescent center in undoped zinc oxide films deposited on silicon substrates , 2001 .

[21]  J. Durig.  Analytical applications of FT-IR to molecular and biological systems : proceedings of the NATO Advanced Study Institute held at Florence, Italy, August 31 to September 12, 1979 [i.e. 1980] , 1980 .

[22]  Su-Hua Yang.  Electrophoretic Prepared ZnGa2 O 4 Phosphor Film for FED , 2003 .

[23]  K. Kempa,et al.  Large hexagonal arrays of aligned ZnO nanorods , 2005 .

[24]  Lin-Wang Wang,et al.  Colloidal nanocrystal heterostructures with linear and branched topology , 2004, Nature.

[25]  W. Xiaoli,et al.  Fabrication of ZnO Nanorod Modified Electrode and Its Application to the Direct Electrochemical Determination of Hemoglobin and Cytochrome c , 2005 .

[26]  Heon-Jin Choi,et al.  Controlled growth of ZnO nanowires and their optical properties , 2002 .

[27]  Xiaoli Zhu,et al.  Electrochemical study of the effect of nano-zinc oxide on microperoxidase and its application to more sensitive hydrogen peroxide biosensor preparation. , 2007, Biosensors & bioelectronics.

[28]  Lin Guo,et al.  Regularly shaped, single-crystalline ZnO nanorods with Wurtzite structure. , 2002, Journal of the American Chemical Society.

[29]  Yiying Wu,et al.  Room-Temperature Ultraviolet Nanowire Nanolasers , 2001, Science.

[30]  M. Yin,et al.  Zinc oxide quantum rods. , 2004, Journal of the American Chemical Society.

[31]  Shui-Tong Lee,et al.  Synthesis of Uniform Hexagonal Prismatic ZnO Whiskers , 2002 .

[32]  Alex Zunger,et al.  Addition Spectra of Quantum Dots: the Role of Dielectric Mismatch , 1999, cond-mat/9908417.

[33]  Lei Zhang,et al.  The electrocatalytic oxidation of ascorbic acid on polyaniline film synthesized in the presence of camphorsulfonic acid , 2004 .