Electrochemical detection of DNA hybridization based on polypyrrole/ss-DNA/multi-wall carbon nanotubes paste electrode.

A sensitive electrochemical detection of DNA hybridization using a paste electrode assembled by multi-wall carbon nanotubes (MWNT) and immobilizing DNA probe within electropolymerized polypyrrole (ppy) was developed. The detection approach relied on entrapping of DNA probe within electropolymerized ppy film on the MWNT paste electrode and monitoring the current change generated from an electroactive intercalator of ethidium bromide (EB) after DNA hybridization. As a consequence of DNA hybridization, significant changes in the current of EB intercalated with double-stranded DNA (ds-DNA) on the MWNT paste electrode were observed. Based on the response of EB, only the complementary DNA sequence gave an obvious current signal compared with the five-point mismatched and non-complementary sequences. The oxidation peak current was linearly related to the logarithm of the concentration of the complementary DNA sequence from 1.0x10(-10) to 1.0x10(-8)M with a detection limit of 8.5x10(-11)M. This work demonstrates that the incorporation of MWNT paste electrode with electropolymerization is a promising strategy of functional interfaces for the immobilization of biological recognition elements.

[1]  A. Erdem,et al.  Interaction of the anticancer drug epirubicin with DNA , 2001 .

[2]  Claude Martelet,et al.  Electrochemical impedance probing of DNA hybridisation on oligonucleotide-functionalised polypyrrole. , 2005, Talanta.

[3]  S. Iijima.  Helical microtubules of graphitic carbon , 1991, Nature.

[4]  Rahul Singhal,et al.  Application of electrochemically prepared polypyrrole-polyvinyl sulphonate films to DNA biosensor. , 2006, Biosensors & bioelectronics.

[5]  Joel S. Silverman,et al.  Detection of Attomole Quantitites of DNA Targets on Gold Microelectrodes by Electrocatalytic Nucleobase Oxidation , 2003 .

[6]  Aiguo Wu,et al.  A method to construct a third-generation horseradish peroxidase biosensor: self-assembling gold nanoparticles to three-dimensional sol-gel network. , 2002, Analytical chemistry.

[7]  Jian Li,et al.  Biocomposites of covalently linked glucose oxidase on carbon nanotubes for glucose biosensor , 2005, Analytical and bioanalytical chemistry.

[8]  Richard J. Coles,et al.  Protein electrochemistry at carbon nanotube electrodes , 1997 .

[9]  Suw Young Ly,et al.  Detection of dopamine in the pharmacy with a carbon nanotube paste electrode using voltammetry. , 2006, Bioelectrochemistry.

[10]  Marek Trojanowicz,et al.  Analytical applications of carbon nanotubes : a review , 2006 .

[11]  A. Erdem,et al.  Rigid carbon composites: a new transducing material for label-free electrochemical genosensing , 2004 .

[12]  Boris Mizaikoff,et al.  Label-free DNA detection based on modified conducting polypyrrole films at microelectrodes. , 2006, Analytical chemistry.

[13]  Ying Xu,et al.  Electrochemical impedance detection of DNA hybridization based on the formation of M-DNA on polypyrrole/carbon nanotube modified electrode , 2004 .

[14]  A. Voulgaropoulos,et al.  Study of interactions between DNA-ethidium bromide (EB) and DNA-acridine orange (AO), in solution, using hanging mercury drop electrode (HMDE). , 2003, Talanta.

[15]  K. M. Millan,et al.  Voltammetric DNA biosensor for cystic fibrosis based on a modified carbon paste electrode. , 1994, Analytical chemistry.

[16]  G. Rivas,et al.  Carbon nanotubes paste electrodes as new detectors for capillary electrophoresis , 2005 .

[17]  T. Malinski,et al.  Nitric oxide release from a single cell measured in situ by a porphyrinic-based microsensor , 1992, Nature.

[18]  Xianting Zeng,et al.  Electrochemical biosensing of DNA with capture probe covalently immobilized onto glassy carbon surface , 2005 .

[19]  Joseph Wang.  Electrochemical nucleic acid biosensors , 2002 .

[20]  Joseph Wang,et al.  Detection of homocysteine at carbon nanotube paste electrodes. , 2004, Talanta.

[21]  X. Xia,et al.  Silica-nanoparticle-based interface for the enhanced immobilization and sequence-specific detection of DNA , 2004, Analytical and bioanalytical chemistry.

[22]  Guodong Liu,et al.  Electrochemical coding technology for simultaneous detection of multiple DNA targets. , 2003, Journal of the American Chemical Society.

[23]  Alain Walcarius,et al.  Study of DNA adsorption on polypyrrole: interest of dielectric monitoring , 2000 .