The efficiency of meiotic recombination between dispersed sequences in Saccharomyces cerevisiae depends upon their chromosomal location.

To examine constrains imposed on meiotic recombination by homologue pairing, we measured the frequency of recombination between mutant alleles of the ARG4 gene contained in pBR322-based inserts. Inserts were located at identical loci on homologues (allelic recombination) or at different loci on either homologous or heterologous chromosomes (ectopic recombination). Ectopic recombination between interstitially located inserts on heterologous chromosomes had an efficiency of 6-12% compared to allelic recombination. By contrast, ectopic recombination between interstitial inserts located on homologues had relative efficiencies of 47-99%. These findings suggest that when meiotic ectopic recombination occurs, homologous chromosomes are already colocalized. The efficiency of ectopic recombination between inserts on homologues decreased as the physical distance between insert sites was increased. This result is consistent with the suggestion that during meiotic recombination, homologues are not only close to each other, but also are aligned end to end. Finally, the efficiency of ectopic recombination between inserts near telomeres (within 16 kb) was significantly greater than that observed with inserts > 50 kb from the nearest telomere. Thus, at the time of recombination, there may be a special relationship between the ends of chromosomes not shared with interstitial regions.

[1]  B. Byers,et al.  Electron microscopic observations on the meiotic karyotype of diploid and tetraploid Saccharomyces cerevisiae. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[2]  M. Bennett.  Premeiotic events and meiotic chromosome pairing. , 1984, Symposia of the Society for Experimental Biology.

[3]  T. Petes,et al.  Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes. , 1986, Genetics.

[4]  M. Dresser,et al.  Meiotic chromosome behavior in spread preparations of yeast , 1988, The Journal of cell biology.

[5]  J. Szostak,et al.  Double-strand breaks at an initiation site for meiotic gene conversion , 1989, Nature.

[6]  T. Petes,et al.  Meiotic recombination between dispersed repeated genes is associated with heteroduplex formation , 1990, Molecular and cellular biology.

[7]  N. Kleckner,et al.  A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae , 1990, Cell.

[8]  J. Loidl.  The initiation of meiotic chromosome pairing: the cytological view. , 1990, Genome.

[9]  J W Szostak,et al.  Detection of heteroduplex DNA molecules among the products of Saccharomyces cerevisiae meiosis. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[10]  G. Roeder,et al.  Meiosis in asynaptic yeast. , 1990, Genetics.

[11]  S. Jinks-Robertson,et al.  Allelic and ectopic interactions in recombination-defective yeast strains. , 1991, Genetics.

[12]  R. Padmore,et al.  Temporal comparison of recombination and synaptonemal complex formation during meiosis in S. cerevisiae , 1991, Cell.

[13]  F. Klein,et al.  Localization of RAP1 and topoisomerase II in nuclei and meiotic chromosomes of yeast , 1992, The Journal of cell biology.

[14]  B. Dujon,et al.  The complete DNA sequence of yeast chromosome III , 1992, Nature.

[15]  C. Janse,et al.  Deletion, insertion and translocation of DNA sequences contribute to chromosome size polymorphism in Plasmodium berghei. , 1992, Memorias do Instituto Oswaldo Cruz.

[16]  E. Gilson,et al.  The positioning of yeast telomeres depends on SIR3, SIR4, and the integrity of the nuclear membrane. , 1993, Cold Spring Harbor symposia on quantitative biology.

[17]  M. Lichten,et al.  Timing of molecular events in meiosis in Saccharomyces cerevisiae: stable heteroduplex DNA is formed late in meiotic prophase , 1993, Molecular and cellular biology.

[18]  N. Kleckner,et al.  Potential advantages of unstable interactions for pairing of chromosomes in meiotic, somatic, and premeiotic cells. , 1993, Cold Spring Harbor symposia on quantitative biology.

[19]  T. Petes,et al.  Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae , 1993, Molecular and cellular biology.

[20]  G. Roeder,et al.  ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis , 1993, Cell.

[21]  J. Jiménez,et al.  Electrophoretic karyotype of budding yeasts with intact cell wall. , 1993, Nucleic acids research.

[22]  T. Wellems,et al.  Interchromosomal exchange of a large subtelomeric segment in a Plasmodium falciparum cross. , 1994, The EMBO journal.

[23]  C. Newlon,et al.  Meiosis-specific formation of joint DNA molecules containing sequences from homologous chromosomes , 1994, Cell.

[24]  N. Kleckner,et al.  Identification of joint molecules that form frequently between homologs but rarely between sister chromatids during yeast meiosis , 1994, Cell.

[25]  Nancy Kleckner,et al.  Chromosome pairing via multiple interstitial interactions before and during meiosis in yeast , 1994, Cell.

[26]  Jonathan A. Cooper,et al.  Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. , 1994, Science.

[27]  J. Ravetch,et al.  The polymorphic subtelomeric regions of Plasmodium falciparum chromosomes contain arrays of repetitive sequence elements. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[28]  H. Scherthan,et al.  Dynamics of chromosome organization and pairing during meiotic prophase in fission yeast , 1994, The Journal of cell biology.

[29]  J. Schimenti,et al.  Gene conversion between unlinked sequences in the germline of mice. , 1994, Genetics.

[30]  M. Lichten,et al.  Factors that affect the location and frequency of meiosis-induced double-strand breaks in Saccharomyces cerevisiae. , 1995, Genetics.

[31]  N. Kleckner,et al.  Identification of double holliday junctions as intermediates in meiotic recombination , 1995, Cell.

[32]  L. New,et al.  Mismatch correction acts as a barrier to homeologous recombination in Saccharomyces cerevisiae. , 1995, Genetics.

[33]  A. Datta,et al.  Mitotic crossovers between diverged sequences are regulated by mismatch repair proteins in Saccaromyces cerevisiae , 1996, Molecular and cellular biology.