Cytochrome oxidase staining marks dendritic zones of the rat olfactory bulb external plexiform layer

Cytochrome oxidase staining of the rat olfactory bulb external plexiform layer (EPL) produces a darkly stained intermediate zone bordered by lightly stained superficial and deep zones. Similar zonal staining was seen in cats, rabbits, and hamsters. These zones vary in relative thickness around the circumference of the olfactory bulb; the deep zone is proportionally thicker in the most dorsal and ventral parts of the bulb. Tufted cell somata are unevenly distributed within the EPL; the outer part of the EPL has more somata. The distribution of the cytochrome oxidase reaction product shows that the darkly stained intermediate zone is not produced by staining of tufted cell somata.

[1]  M. Wong-Riley,et al.  The histochemical localization of cytochrome oxidase in the retina and lateral geniculate nucleus of the ferret, cat, and monkey, with particular reference to retinal mosaics and ON/OFF-center visual channels , 1984, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[2]  Lewis B. Haberly,et al.  The axonal projection patterns of the mitral and tufted cells of the olfactory bulb in the rat , 1977, Brain Research.

[3]  A. L. Humphrey,et al.  Background and stimulus-induced patterns of high metabolic activity in the visual cortex (area 17) of the squirrel and macaque monkey , 1983, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[4]  S. P. Schneider,et al.  Orthodromic response properties of rat olfactory bulb mitral and tufted cells correlate with their projection patterns. , 1983, Journal of neurophysiology.

[5]  W. C. Hall,et al.  Efferent projections of the main and the accessory olfactory bulb in the tree shrew (Tupaia glis) , 1977, The Journal of comparative neurology.

[6]  D. Hubel,et al.  Anatomy and physiology of a color system in the primate visual cortex , 1984, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[7]  E Orona,et al.  Dendritic and axonal organization of mitral and tufted cells in the rat olfactory bulb , 1984, The Journal of comparative neurology.

[8]  K Kishi,et al.  Distribution of local axon collaterals of mitral, displaced mitral, and tufted cells in the rabbit olfactory bulb , 1984, The Journal of comparative neurology.

[9]  F R Sharp,et al.  Laminar analysis of 2-deoxyglucose uptake in olfactory bulb and olfactory cortex of rabbit and rat. , 1977, Journal of neurophysiology.

[10]  G. Shepherd,et al.  Synaptic actions on mitral and tufted cells elicited by olfactory nerve volleys in the rabbit. , 1975, The Journal of physiology.

[11]  E Orona,et al.  Different granule cell populations innervate superficial and deep regions of the external plexiform layer in rat olfactory bulb , 1983, The Journal of comparative neurology.

[12]  F. Macrides,et al.  Topographic organization of tufted cell axonal projections in the hamster main olfactory bulb: An intrabulbar associational system , 1985, The Journal of comparative neurology.

[13]  F. Macrides,et al.  Evidence for morphologically, neurochemically and functionally heterogeneous classes of mitral and tufted cells in the olfactory bulb , 1985 .

[14]  K. Mori.  Membrane and synaptic properties of identified neurons in the olfactory bulb , 1987, Progress in Neurobiology.

[15]  M. Wong-Riley,et al.  An analysis of the cellular localization of cytochrome oxidase in the lateral geniculate nucleus of the adult cat , 1985, The Journal of comparative neurology.

[16]  J. G. Parnavelas,et al.  The reciprocal synapse in the external plexiform layer of the mammalian olfactory bulb , 1978, Brain Research.

[17]  F. Macrides,et al.  Laminar organization of mitral and tufted cells in the main olfactory bulb of the adult hamster , 1982, The Journal of comparative neurology.

[18]  F. Macrides,et al.  Tyrosine hydroxylase immunoreactive neurons and fibers in the olfactory system of the hamster , 1983 .

[19]  D. Hubel,et al.  Regular patchy distribution of cytochrome oxidase staining in primary visual cortex of macaque monkey , 1981, Nature.

[20]  J. Adams.  Heavy metal intensification of DAB-based HRP reaction product. , 1981, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[21]  F. Macrides,et al.  Ultrastructural identification of substance P immunoreactive neurons in the main olfactory bulb of the hamster , 1982, Neuroscience.

[22]  M. Wong-Riley,et al.  Quantitative light and electron microscopic analysis of cytochrome oxidase‐rich zones in the striate cortex of the squirrel monkey , 1984, The Journal of comparative neurology.

[23]  T. Powell,et al.  The neuron types of the glomerular layer of the olfactory bulb. , 1971, Journal of cell science.

[24]  W. R. Adey.  The synaptic organization of the brain. 2nd edn. , 1981 .

[25]  R B Tootell,et al.  Topography of cytochrome oxidase activity in owl monkey cortex , 1985, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[26]  C. Gall,et al.  Distribution of cholecystokinin‐like immunoreactivity in the rat main olfactory bulb , 1985, The Journal of comparative neurology.

[27]  H. Ojima,et al.  The trajectory of mitral cell axons in the rabbit olfactory cortex revealed by intracellular HRP injection , 1984, The Journal of comparative neurology.

[28]  T. Powell,et al.  The mitral and short axon cells of the olfactory bulb. , 1970, Journal of cell science.

[29]  T. Joh,et al.  Transneuronal regulation of tyrosine hydroxylase expression in olfactory bulb of mouse and rat , 1983, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[30]  J S Kauer,et al.  Mapping of odor-related neuronal activity in the olfactory bulb by high-resolution 2-deoxyglucose autoradiography. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[31]  M. Gershon,et al.  Functional heterogeneity in the myenter is plexus: Demonstration using cytochrome oxidase as a verified cytochemical probe of the activity of individual enteric neurons , 1986, The Journal of comparative neurology.

[32]  M. Wong-Riley.  Changes in the visual system of monocularly sutured or enucleated cats demonstrable with cytochrome oxidase histochemistry , 1979, Brain Research.

[33]  C. Greer,et al.  Golgi analyses of dendritic organization among denervated olfactory bulb granule cells , 1987, The Journal of comparative neurology.

[34]  J. W. Scott,et al.  The organization of projections from the olfactory bulb to the piriform cortex and olfactory tubercle in the rat , 1980, The Journal of comparative neurology.

[35]  L. Astic,et al.  Spatial distribution of [14C]2-deoxyglucose uptake in the olfactory bulbs of rats stimulated with two different odours , 1980, Brain Research.

[36]  F. Macrides,et al.  Topographic organization of connections between the main olfactory bulb and pars externa of the anterior olfactory nucleus in the hamster , 1984, The Journal of comparative neurology.

[37]  G. Shepherd,et al.  Functional organization of rat olfactory bulb analysed by the 2‐deoxyglucose method , 1979, The Journal of comparative neurology.

[38]  J. Scott.  Electrophysiological identification of mitral and tufted cells and distributions of their axons in olfactory system of the rat. , 1981, Journal of neurophysiology.

[39]  K Kishi,et al.  Distribution of dendrites of mitral, displaced mitral, tufted, and granule cells in the rabbit olfactory bulb , 1983, The Journal of comparative neurology.