Avaliação da técnica de coloração AgNOR em testículos de ovinos

The silver staining technique for AgNOR nucleolar organizer regions (NORs) is characterized by marking proteins linked to the ribosomal ribonucleic acid, evaluating cell proliferation. The aim was to study the validity of the AgNOR staining technique in the testicular cell proliferation in crossbred ovine. Forty-three pairs of ovine testicles between 6 and 10 months old were collected. Wilcoxon and Spearman tests were used with a significance level of 5%. The mean NORs count in cells of the right and left gonads were respectively: spermatogonia (8.77±1.14 and 9.04±0.96), spermatocytes (4.99±2.00 and 6.20±2.07, P<0.05), Leydig (8.05±2.82 and 7.89±2.29) and Sertoli cells (8.07±1.88 and 7.61±2.16; P<0.05). There was a correlation between the mean values for the right and left sides for the number of NORs (P<0.05) between Leydig x spermatocytes (0.49); spermatocytes x Sertoli (0.49) and Sertoli x Leydig (0.96). The study demonstrates that the AgNOR staining technique is indicated to evaluate the cell proliferative potential in ovine testis with practical implementation and low cost.

[1]  Elena Riet-Correa Rivero,et al.  Avaliação do metabolismo epitelial em cistos radiculares pela técnica de AgNORS , 2014 .

[2]  M. Rodrigues,et al.  The lowest diploid number in Testudines: Banding patterns, telomeric and 45S rDNA FISH in Peltocephalus dumerilianus, 2n = 26 and FN = 52 (Pleurodira, Podocnemididae) , 2013, Genetics and molecular biology.

[3]  A. Gurgul,et al.  Changes in DNA methylation patterns and repetitive sequences in blood lymphocytes of aged horses , 2013, AGE.

[4]  M. G. Chacur.  Seminal Plasma Proteins as Potential Markers of Relative Fertility in Zebu Bulls (Bos taurus indicus) , 2012 .

[5]  E. Oba,et al.  Influência da estação do ano nas características do sêmen e na concentração de hormônios em touros Nelore e Simental , 2012 .

[6]  F. Santos,et al.  Marcadores de proliferação celular no tumor venéreo transmissível canino transplantado , 2011 .

[7]  T. Mott,et al.  Karyological study of Amphisbaena ridleyi (Squamata, Amphisbaenidae), an endemic species of the Archipelago of Fernando de Noronha, Pernambuco, Brazil , 2010, Genetics and molecular biology.

[8]  C. Rezende,et al.  Efeitos do sulfato de condroitina e do hialuronato de sódio nos condrócitos e na matriz extracelular na cartilagem articular de cães com doença articular degenerativa , 2008 .

[9]  K. Schmid,et al.  Standardized AgNOR analysis: its usefulness in surgical oncology , 1996, Histochemistry and Cell Biology.

[10]  B. Madewell.  Cellular proliferation in tumors: a review of methods, interpretation, and clinical applications. , 2001, Journal of veterinary internal medicine.

[11]  L. Chiusa,et al.  Prognostic relevance of AgNORs in tumor pathology. , 2000, Micron.

[12]  J. Zheng,et al.  [Video image analysis of AgNORs distribution in the testicular tumor]. , 1997, Zhonghua wai ke za zhi [Chinese journal of surgery].

[13]  H. Lauke,et al.  Histologic Diagnostics of Early Testicular Germ‐Cell Tumor , 1996, International journal of urology : official journal of the Japanese Urological Association.

[14]  C. Ohyama,et al.  [Clinical significance of proliferating cell nuclear antigen (PCNA) and argyrophilic nucleolar organizer region (AgNOR) in testicular tumors]. , 1995, Nihon Hinyokika Gakkai zasshi. The japanese journal of urology.

[15]  H. Rodríguez-Martínez,et al.  Onset of Spermatogenesis in Corriedale Ram Lambs under Extensive Rearing Conditions in Uruguay , 1995, Acta Veterinaria Scandinavica.

[16]  A. Harmelin,et al.  Correlation of Ag-NOR protein measurements with prognosis in canine transmissible venereal tumour. , 1995, Journal of comparative pathology.

[17]  S. Ergün,et al.  Segmental angioarchitecture of the testicular lobule in man , 2009, Andrologia.

[18]  H. Lauke,et al.  The value of the AgNOR staining method in identifying carcinoma in situ testis. , 1994, Pathology, research and practice.

[19]  G. De Vico,et al.  Number and size of silver-stained nucleoli (Ag-NOR clusters) in canine seminomas: correlation with histological features and tumour behaviour. , 1994, Journal of comparative pathology.

[20]  U. Agrimi,et al.  Nucleolar size and mitotic index in basal cell carcinomas (BCC) and squamous cell carcinomas (SCC) of canine skin. , 1994, Zentralblatt fur Veterinarmedizin. Reihe A.

[21]  L. Lindner.  Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR). , 1993, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[22]  D. Boldy,et al.  How should we count AgNORS? Proposals for a standardized approach , 1989, The Journal of pathology.