Application of Films Based on Chitosan and Xanthan Gum in Refrigerated Fish Conservation

: This research aims to determine the efficiency of chitosan and xanthan gum films in conservation of croaker fillets kept in refrigeration for 9 days. Proximal composition, loss of mass, color, pH, TVB-N (Total Volatile Bases) and microbiological profile were assessed. The films were prepared with chitosan and xanthan gum in varying mass proportions 100:0, m:m (C100XG0); 60:40, m:m (C60XG40); 50:50, m:m (C50XG50). They presented the respective values for moisture content, water solubility, thickness and water vapor permeability: 24.59%, 19.50%, 0.086 mm and 11.45gm -1 .s -1 .Pa -1 for C100XG0; 24.58%; 20.27%, 0.091 mm and 10.41 gm -1 .s -1 .Pa -1 for C60XG40; 22.11%, 22.06%, 0.089 mm and 10.68 gm -1 .s -1 .Pa -1 forC50XG50.The films were made in small bags format capable to hold about 20 g of fish fillets. A control sample was prepared in parallel, using polyethylene bags under the same storage conditions. The results showed that the chitosan films combined with xanthan gum had excellent antimicrobial properties, capable of preserving the quality of chilled fish fillets during the studied period, since it inhibited the growth of Staphylococcus coagulase-positive, Salmonella spp and coliforms at 45 ° C. Mass loss of the croaker fillets was not significantly affected by xanthan gum addition to the films. On the other hand, xanthan gum addition affected pH and color parameters of the corvina fillets. It was also verified that the combination of these two polymers promoted the reduction of N-BVT, being the C50XG50 film that presented the best response.

[1]  Sabu Thomas,et al.  Mechanism of phase separation in a weakly interacting system with strong dynamic asymmetry , 2017 .

[2]  E. Zavareze,et al.  Biodegradable films based on chitosan, xanthan gum, and fish protein hydrolysate , 2017 .

[3]  E. Zavareze,et al.  Structural, Thermal, Physical, Mechanical, and Barrier Properties of Chitosan Films with the Addition of Xanthan Gum. , 2017, Journal of food science.

[4]  G. Corção,et al.  Characterization of the variable region in the class 1 integron of antimicrobial-resistant Escherichia coli isolated from surface water , 2016, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology].

[5]  W. A. Johnston Freezing and refrigerated storage in fisheries , 2016 .

[6]  A. Plepis,et al.  Influence of collagen addition on the thermal and morphological properties of chitosan/xanthan hydrogels. , 2015, International journal of biological macromolecules.

[7]  Mirari Y. Arancibia,et al.  Chitosan coatings enriched with active shrimp waste for shrimp preservation , 2015 .

[8]  A. Vicente,et al.  Effects of glazing and chitosan-based coating application on frozen salmon preservation during six-month storage in industrial freezing chambers , 2015 .

[9]  M. Zarei,et al.  Comparing the effectiveness of chitosan and nanochitosan coatings on the quality of refrigerated silver carp fillets , 2015 .

[10]  C. Boeriu,et al.  Chitosan films and blends for packaging material. , 2015, Carbohydrate polymers.

[11]  S. Sathivel,et al.  Evaluation of chitosan nanoparticles as a glazing material for cryogenically frozen shrimp , 2014 .

[12]  Carlos Prentice,et al.  Using edible coatings from Whitemouth croaker (Micropogonias furnieri) protein isolate and organo-clay nanocomposite for improve the conservation properties of fresh-cut ‘Formosa’ papaya , 2014 .

[13]  Shengjun Wu Effect of chitosan-based edible coating on preservation of white shrimp during partially frozen storage. , 2014, International journal of biological macromolecules.

[14]  A. Chiralt,et al.  Physical, structural and antimicrobial properties of poly vinyl alcohol-chitosan biodegradable films , 2014 .

[15]  A. Vicente,et al.  Effect of chitosan-based solutions applied as edible coatings and water glazing on frozen salmon preservation – A pilot-scale study , 2013 .

[16]  C. Conte‐Junior,et al.  Quality Index Method (QIM) developed for pacu Piaractus mesopotamicus and determination of its shelf life , 2013 .

[17]  M. Elsabee,et al.  Chitosan based edible films and coatings: a review. , 2013, Materials science & engineering. C, Materials for biological applications.

[18]  P. Williams,et al.  Control of the properties of xanthan/glucomannan mixed gels by varying xanthan fine structure. , 2013, Carbohydrate polymers.

[19]  O. Sauperl,et al.  Chitosan coatings onto polyethylene terephthalate for the development of potential active packaging material , 2013 .

[20]  J. Vieites,et al.  Whey protein-based coatings on frozen Atlantic salmon (Salmo salar): Influence of the plasticiser and the moment of coating on quality preservation. , 2011, Food chemistry.

[21]  A. Mauri,et al.  Addition of bovine plasma hydrolysates improves the antioxidant properties of soybean and sunflower protein-based films , 2011 .

[22]  Arun Sharma,et al.  Chitosan and guar gum composite films: Preparation, physical, mechanical and antimicrobial properties , 2010 .

[23]  J. Gómez-Estaca,et al.  Biodegradable gelatin-chitosan films incorporated with essential oils as antimicrobial agents for fish preservation. , 2010, Food microbiology.

[24]  P. Sobral,et al.  Development of films based on quinoa (Chenopodium quinoa, Willdenow) starch , 2010 .

[25]  K. Neoh,et al.  Antioxidant and antibacterial activities of eugenol and carvacrol‐grafted chitosan nanoparticles , 2009, Biotechnology and bioengineering.

[26]  Yuanlong Chi,et al.  Effects of chitosan coating on quality and shelf life of silver carp during frozen storage , 2009 .

[27]  E. Küçüköner,et al.  Effect of edible coatings on the quality of frozen fish fillets , 2009 .

[28]  L. L. Nesse,et al.  Salmonella in fish feed; occurrence and implications for fish and human health in Norway , 2007 .

[29]  W. Visessanguan,et al.  Changes of pigments and color in sardine (Sardinella gibbosa) and mackerel (Rastrelliger kanagurta) muscle during iced storage , 2005 .

[30]  B. Harris Health by association. , 2005, International journal of epidemiology.

[31]  A. J. Alves,et al.  Mechanical properties, hydrophilicity and water activity of starch-gum films: effect of additives and deacetylated xanthan gum , 2005 .

[32]  M. Rafiee-Tehrani,et al.  Diethylmethyl chitosan as an antimicrobial agent: Synthesis, characterization and antibacterial effects , 2004 .

[33]  G. Sampaio,et al.  The influence of season on the lipid profiles of five commercially important species of Brazilian fish , 2003 .

[34]  Vasiliki R. Kyrana,et al.  Assessment of shelf-life of maricultured gilthead sea bream (Sparus aurata) stored in ice , 1997 .

[35]  B. Chiang,et al.  ANTIMICROBIAL AND PHYSICOCHEMICAL PROPERTIES OF METHYLCELLULOSE AND CHITOSAN FILMS CONTAINING A PRESERVATIVE , 1996 .

[36]  C. Vanderzant,et al.  Compendium of Methods for the Microbiological Examination of Foods , 1992 .

[37]  M. Rhodes,et al.  Survival of Escherichia coli and Salmonella spp. in estuarine environments , 1988, Applied and environmental microbiology.

[38]  M. Rezaii of Official Analytical Chemists, , 2017 .

[39]  M. Das,et al.  A Comparative Microbiological Assessment of Five Types of Selected Fishes Collected from Two Different Market , 2010 .

[40]  M. C. Lanari,et al.  Atmosphere and blooming time affect color and lipid stability of frozen beef from steers supplemented with Vitamin E. , 1995, Meat science.

[41]  V. Isaac Synopsis of biological data on the whitemouth croaker Micropogonias furnieri (Desmarest, 1823) , 1988 .