EUPERGIT oxirane acrylic beads: How to make enzymes fit for biocatalysis

Enzyme recycling is essential for the development of large-scale enzyme-catalyzed biotransformations. Recycling is most convenient using enzymes immobilized on solid supports. Although immobilization on solid supports has been pursued since the 1950s, there are no general rules for selecting the best support for a given application. The commercial products EUPERGIT C and EUPERGIT C 250 L have been used for a wide variety of different enzymes and reactions. The present review draws up a comprehensive application profile of both EUPERGIT carriers. The reader gets (a) examples of biotransformations using oxidoreductases, transferases, hydrolases and lyases immobilized on EUPERGIT; (b) key data of the biotransformations, i.e., scale, yield, purity, and enantiomeric excess; (c) efficiency of the immobilization (% immobilized activity); (d) where appropriate, operational stability of the immobilized enzyme preparations, that is, number of cycles, residual activity; (e) specific advantages of the immobilized enz...