论文信息 - Application of femtosecond laser ablation for detaching grown protein crystals from glass capillary tube.

Application of femtosecond laser ablation for detaching grown protein crystals from glass capillary tube.

We developed a novel technique for detaching protein crystals from glass capillary tube using the counter diffusion crystallization technique by femtosecond laser irradiation. X-ray diffraction analysis demonstrated that femtosecond laser irradiation has little effect on crystallinity. This technique will contribute to progress in structural genomics as a powerful tool.

[1] L. Gonzalez-Ramirez,et al. Granada Crystallisation Box: a new device for protein crystallisation by counter-diffusion techniques. , 2002, Acta crystallographica. Section D, Biological crystallography.

[2] Tsuyoshi Inoue,et al. Application of UV-Laser Ablation to Detaching Protein Crystal from Growth Vessel , 2004 .

[3] J. García-Ruiz,et al. Investigations on protein crystal growth by the gel acupuncture method. , 1994, Acta crystallographica. Section D, Biological crystallography.

[4] G. Mourou,et al. Terawatt to Petawatt Subpicosecond Lasers , 1994, Science.

[5] Hiroshi Masuhara,et al. Femtosecond Laser Processing of Protein Crystals in Crystallization Drop , 2005 .

[6] Kenichi Kojima,et al. Growth and mechanical properties of lysozyme crystals , 1999 .

[7] A. McPherson,et al. Crystallization of biological macromolecules from flash frozen samples on the Russian Space Station Mir , 1996, Biotechnology and bioengineering.

[8] S. Sano,et al. A simplified counter diffusion method combined with a 1D simulation program for optimizing crystallization conditions. , 2004, Journal of synchrotron radiation.