Antibacterial Activity of Synthetic Peptides Against Plant Pathogenic Pectobacterium Species

The aim of the work was to check the antibacterial activity of three synthetic peptides: CAMEL, Iseganan and Pexiganan as well as their possible application against plant pathogenic bacteria from the species Pectobacterium carotovorum (Pc) and Pectobacterium chrysanthemi (Pch). The antibacterial activity of the three chosen synthetic peptides was evaluated with the use of two tests: minimal inhibitory concentration and minimal bactericidal concentration. The CAMEL proved to be the most effective peptide, inhibiting the growth of different species of Pectobacterium in concentrations ranging from 2 to 8 μg/ml. Iseganan and Pexiganan also demonstrated activity against Pectobacterium sp., but it was lower than CAMEL. The CAMEL was able to inhibit Pc and Pch bacterial growth and tissue maceration in pathogenicity tests performed on potato tuber slices.

[1]  R. Nazar,et al.  Plant defense gene promoter enhances the reliability of shiva-1 gene-induced resistance to soft rot disease in potato , 2004, Planta.

[2]  A. Fraser,et al.  Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[3]  J. Gauthier,et al.  Characterisation of Erwinia carotovora subspecies and detection of Erwinia carotovora subsp. atroseptica in potato plants, soil and water extracts with PCR-based methods , 1998, European Journal of Plant Pathology.

[4]  W. Stiekema,et al.  Erwinia soft rot resistance of potato cultivars expressing antimicrobial peptide tachyplesin I , 1996, Molecular Breeding.

[5]  K. Bogucka,et al.  Activities of synthetic peptides against human pathogenic bacteria. , 2004, Polish journal of microbiology.

[6]  R. Buddington,et al.  A kinetic microplate method for quantifying the antibacterial properties of biological fluids. , 2003, Journal of microbiological methods.

[7]  R. Christen,et al.  Elevation of three subspecies of Pectobacterium carotovorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov. , 2003, International journal of systematic and evolutionary microbiology.

[8]  H. El-Hendawy,et al.  Pectic Enzymes Produced In vitro and In vivo by Erwinia spp. Isolated from Carrot and Pepper in Egypt , 2002 .

[9]  R. Lehrer,et al.  Potassium release, a useful tool for studying antimicrobial peptides. , 2002, Journal of microbiological methods.

[10]  K. Tsuchiya,et al.  Phenotypic and genetic diversity of Erwinia carotovora ssp. carotovora strains from Asia , 2002 .

[11]  M. Zasloff Antimicrobial peptides of multicellular organisms , 2002, Nature.

[12]  B. Kochańska,et al.  Statherin SV2 and its analogue. Synthesis and evaluation of antimicrobial activity , 2002 .

[13]  Guoqing Zhou,et al.  Transgenic plants expressing cationic peptide chimeras exhibit broad-spectrum resistance to phytopathogens , 2000, Nature Biotechnology.

[14]  G. S. Ali,et al.  Inhibition of fungal and bacterial plant pathogens by synthetic peptides: in vitro growth inhibition, interaction between peptides and inhibition of disease progression. , 2000, Molecular plant-microbe interactions : MPMI.

[15]  T. Falla,et al.  IB-367, a Protegrin Peptide with In Vitro and In Vivo Activities against the Microflora Associated with Oral Mucositis , 2000, Antimicrobial Agents and Chemotherapy.

[16]  H. Oh,et al.  Activities of Synthetic Hybrid Peptides against Anaerobic Bacteria: Aspects of Methodology and Stability , 2000, Antimicrobial Agents and Chemotherapy.

[17]  J. Fiddes,et al.  Development of protegrins for the treatment and prevention of oral mucositis: structure-activity relationships of synthetic protegrin analogues. , 2000, Biopolymers.

[18]  K. Richter,et al.  NOURSEOTHRICIN - A NEW AGENT FOR CONTROL OF FIRE BLIGHT , 1999 .

[19]  K. Holroyd,et al.  In Vitro Antibacterial Properties of Pexiganan, an Analog of Magainin , 1999, Antimicrobial Agents and Chemotherapy.

[20]  J. Mergaert,et al.  Phylogenetic position of phytopathogens within the Enterobacteriaceae. , 1998, Systematic and applied microbiology.

[21]  Steven D. Brown,et al.  In Vitro Antimicrobial Activity of MSI-78, a Magainin Analog , 1998, Antimicrobial Agents and Chemotherapy.

[22]  E. Titarenko,et al.  Mutants of Ralstonia (Pseudomonas) solanacearum sensitive to antimicrobial peptides are altered in their lipopolysaccharide structure and are avirulent in tobacco , 1997, Journal of bacteriology.

[23]  S. Kwon,et al.  Phylogenetic analysis of Erwinia species based on 16S rRNA gene sequences. , 1997, International journal of systematic bacteriology.

[24]  A. Kotoujansky,et al.  Characterization of Erwinia chrysanthemi by pectinolytic isozyme polymorphism and restriction fragment length polymorphism analysis of PCR-amplified fragments of pel genes , 1996, Applied and environmental microbiology.

[25]  J. Gabay,et al.  Ubiquitous natural antibiotics. , 1994, Science.

[26]  J. Jaynes,et al.  Expression of a Cecropin B lytic peptide analog in transgenic tobacco confers enhanced resistance to bacterial wilt caused by Pseudomonas solanacearum , 1993 .

[27]  L. Owens,et al.  Activity of cecropin SB37 against protoplasts from several plant species and their bacterial pathogens , 1992 .

[28]  G. Fields,et al.  Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids. , 2009, International journal of peptide and protein research.

[29]  and M C M Perombelon,et al.  Ecology of the Soft Rot Erwinias , 1980 .

[30]  L. Thornell,et al.  A Qualitative Test for Monitoring Coupling Completeness in Solid Phase Peptide Synthesis Using Chloranil. , 1979 .