Staphylococcal Protein A in Immunoferritin Techniques

Protein A (pA) of Staphylococcus aureus, by virtue of its reactivity with the Fc portion of a variety of mammalian immunoglobulins (IgG), can be used for electron‐microscopical immunoferritin technique. The conjugation of pA with ferritin (pA‐F) by means of bis (4 fluoro‐3‐nitrophenyl) sulfone results in a highly specific marker for IgG in indirect labeling experiments. Native pA can be used as a noncovalent bridging agent between Specific antibodies and antiferritin capturing the marker molecule. The use of pA and pA‐F is demonstrated with a model system involving human erythrocytes and twO rabbit antisera directed against different surface antigens of the human erythrocyte membrane.

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