Changes in Activities of Superoxide Dismutase, Catalase and Peroxidase during Senescence of Gladiolus Florets

Treatments with sodium benzoate and n-propyl gallate as free radical scavengers slightly delayed the wilting of perianth in detached gladiolus florets. Superoxide dismutase (SOD) activity of the perianths sharply decreased in 2 days after their full unfolding (DAU) when they commenced to wilt. The decrease of SOD activity was alleviated by treatments with 300μM cycloheximide (CHI) which prevented perianths from wilting. Specific activity of catalase (CAT) of perianths did not markedly change, but CAT activity on the basis of perianth decreased concomitant with their wilting. CHI slightly decreased specific activity of CAT. Peroxidase (POD) activity of the perianths sharply increased 1 DAU. This increase was almost completely suppressed by CHI treatments. These results indicate that the free radicals are involved with the decline of SOD activity, increase of POD activity, and in the wilting process of gladiolus perianths.

[1]  T. Panavas,et al.  Oxidative events during programmed cell death of daylily (Hemerocallis hybrid) petals , 1998 .

[2]  R. McConchie,et al.  CHARACTERISTICS OF PETAL SENESCENCE IN A NON-CLIMACTERIC CUT FLOWER , 1995 .

[3]  K. Yamane,et al.  EXPORT OF 14C-SUCROSE, 3H-WATER, AND FLUORESCENT TRACERS FROM GLADIOLUS FLORETS TO OTHER PLANT PARTS ASSOCIATED WITH SENESCENCE , 1995 .

[4]  W. Doorn,et al.  Solute leakage, lipid peroxidation, and protein degradation during the senescence of Iris tepals , 1995 .

[5]  K. Yamane,et al.  Effects of Cycloheximide on Physiological Parameters of Gladiolus Floretsduring Growth and Senescence , 1995 .

[6]  C. Wang.  Effect of temperature preconditioning on catalase, peroxidase, and superoxide dismutase in chilled zucchini squash , 1995 .

[7]  M. Reid,et al.  The Effect of Protein Synthesis Inhibition on Petal Senescence in Cut Bulb Flowers , 1994 .

[8]  M. Reid,et al.  Role of Ethylene in Opening and Senescence of Gladiolus sp. Flowers , 1994 .

[9]  K. Yamane,et al.  Export of Soluble Sugars and Increase in Membrane Permeability of Gladiolus Florets during Senescence , 1993 .

[10]  M. Reid,et al.  BULB-TYPE FLOWER SENESCENCE , 1989 .

[11]  R. Crawford,et al.  Oxygen toxicity and superoxide dismutase as an antioxidant in physiological stress , 1989 .

[12]  A. Paulin,et al.  Changes in activities of superoxide dismutases during aging of petals of cut carnations (Dianthus caryophyllus) , 1989 .

[13]  A. Paulin,et al.  Free radical production, catalase and superoxide dismutase activities and membrane integrity during senescence of petals of cut carnations (Dianthus caryophyllus) , 1987 .

[14]  A. Paulin,et al.  Effect of a free radical scavenger, 3,4,5‐trichlorophenol, on ethylene production and on changes in lipids and membrane integrity during senescence of petals of cut carnations (Dianthus caryophyllus) , 1986 .

[15]  R. Legge,et al.  Superoxide radical production by microsomal membranes from senescing carnation flowers: an effect on membrane fluidity , 1983 .

[16]  R. Dhindsa,et al.  Leaf Senescence: Correlated with Increased Levels of Membrane Permeability and Lipid Peroxidation, and Decreased Levels of Superoxide Dismutase and Catalase , 1981 .

[17]  J. E. Baker,et al.  Delay of Senescence in Carnations by a Rhizobitoxine Analog and Sodium Benzoate1 , 1977, HortScience.

[18]  B. Chance,et al.  The assay of catalases and peroxidases. , 2006, Methods of biochemical analysis.

[19]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.