Establishment of a Real-Time PCR-Based Approach for Accurate Quantification of Bacterial RNA Targets in Water, Using Salmonella as a Model Organism
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Sébastien Flavier | Richard Christen | Carlos A. Guzmán | M. Höfle | R. Christen | C. Guzmán | Manfred G. Höfle | Axel Fey | Stefan Eichler | S. Flavier | A. Fey | S. Eichler
[1] M. Pfaffl,et al. Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR , 2001, Biotechnology Letters.
[2] R. Phadke,et al. Computational methods and evaluation of RNA stabilization reagents for genome-wide expression studies. , 2003, Journal of microbiological methods.
[3] Marta Hernández,et al. A rapid and direct real time PCR-based method for identification of Salmonella spp. , 2003, Journal of microbiological methods.
[4] R. Amann,et al. Quantification of dissimilatory (bi)sulphite reductase gene expression in Desulfobacterium autotrophicum using real-time RT-PCR. , 2003, Environmental microbiology.
[5] L Birch,et al. Molecular methods for the assessment of bacterial viability. , 2003, Journal of microbiological methods.
[6] D. Chandler,et al. Towards a unified system for detecting waterborne pathogens. , 2003, Journal of microbiological methods.
[7] Reiner Helmuth,et al. Multicenter Validation of the Analytical Accuracy of Salmonella PCR: towards an International Standard , 2003, Applied and Environmental Microbiology.
[8] J. Paul,et al. Real-Time PCR Quantification of rbcL (Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase) mRNA in Diatoms and Pelagophytes , 2002, Applied and Environmental Microbiology.
[9] M. Chung,et al. Persistent DNA contamination in competitive RT-PCR using cRNA internal standards: identity, quantity, and control. , 2002, BioTechniques.
[10] Nigel J. Walker,et al. A Technique Whose Time Has Come , 2002, Science.
[11] M. Van Ranst,et al. Use of gDNA as internal standard for gene expression in staphylococci in vitro and in vivo. , 2002, Biochemical and biophysical research communications.
[12] M. Weinbauer,et al. Simultaneous Extraction from Bacterioplankton of Total RNA and DNA Suitable for Quantitative Structure and Function Analyses , 2002, Applied and Environmental Microbiology.
[13] W. Peetermans,et al. Quantification of Expression of Staphylococcus epidermidis Housekeeping Genes with Taqman Quantitative PCR during In Vitro Growth and under Different Conditions , 2001, Journal of bacteriology.
[14] R. Wilson,et al. Complete genome sequence of Salmonella enterica serovar Typhimurium LT2 , 2001, Nature.
[15] B. Guy,et al. Assessment of Helicobacter pylori Gene Expression within Mouse and Human Gastric Mucosae by Real-Time Reverse Transcriptase PCR , 2001, Infection and Immunity.
[16] M. Pfaffl,et al. A new mathematical model for relative quantification in real-time RT-PCR. , 2001, Nucleic acids research.
[17] S. Bustin. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. , 2000, Journal of molecular endocrinology.
[18] T. Jemmi,et al. PCR Detection, Characterization, and Distribution of Virulence Genes in Aeromonas spp , 1999, Applied and Environmental Microbiology.
[19] V. Sharma,et al. Semi-automated fluorogenic PCR assays (TaqMan) forrapid detection of Escherichia coli O157:H7 and other shiga toxigenic E. coli. , 1999, Molecular and cellular probes.
[20] S. Walker,et al. Quantitative RT-PCR : Pitfalls and Potential , 1999 .
[21] C. Byrne,et al. Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR. , 1999, The Biochemical journal.
[22] H. Bremer. Modulation of Chemical Composition and Other Parameters of the Cell by Growth Rate , 1999 .
[23] B. Mackey,et al. Detection of mRNA by Reverse Transcription-PCR as an Indicator of Viability in Escherichia coliCells , 1998, Applied and Environmental Microbiology.
[24] J. Paton,et al. Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. , 1998, Journal of clinical microbiology.
[25] R. Gourse,et al. rRNA transcription and growth rate-dependent regulation of ribosome synthesis in Escherichia coli. , 1996, Annual review of microbiology.
[26] R. Christen,et al. Assessment of the state of activity of individual bacterial cells by hybridization with a ribosomal RNA targeted fluorescently labelled oligonucleotidic probe , 1994 .
[27] N. Kjeldgaard,et al. Regulation of Biosynthesis of Ribosomes , 1974 .